Concerning the role of endogenous copper in the acute inflammatory process

Milanino, R.; Conforti, Anita; Fracasso, Maria Enrica; Franco, L.; Leone, Roberto; Passarella, E; Tarter, G.; Velo, Giampaolo

doi:10.1007/bf01968131

Cited by 37 publications

(10 citation statements)

References 27 publications

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“…This hypothesis received further support from our studies on copper-deficient rats. We showed that a severe copper-deficiency produces a statistically significant increase of the foot swelling and of the pleural exudate volume following carrageenan injections [21,25]. Our results were recently confirmed by DENKO [26].…”

Section: Introductionsupporting

confidence: 91%

“…As pointed out earlier in this paper the induction of a copperdeficiency status produces an exacerbation of the inflammatory response in the rat. It is now relevant to stress that these copper-deprived rats are likely to be less protected from free radical damage than are normal animals, since copperdeficiency may also induce a significant decrease of both ceruloplasmin [21,25,39] …”

Section: Hours After the Irritantmentioning

confidence: 99%

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Copper and ceruloplasmin (Cp) concentrations during the acute inflammatory process in the rat

et al. 1982

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We have measured the concentration of copper and ceruloplasmin in serum of rats with carrageenan foot-oedema and in serum and exudate of rats with carrageenan pleurisy. Both serum copper and ceruloplasmin were found to increase in both models of inflammation. In these experimental conditions we have shown a strong positive correlation between copper and ceruloplasmin, in the serum of both normal and inflamed rats.

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Section: Introductionsupporting

confidence: 91%

Section: Hours After the Irritantmentioning

confidence: 99%

Copper and ceruloplasmin (Cp) concentrations during the acute inflammatory process in the rat

et al. 1982

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“…We have shown that copper-deficient rats are more prone to the effects of standard acute inflammatory agents compared with animals on a normal copper diet (Milanino, Mazzoli, Passarella, Tarter & Velo, 1978;Milanino, Conforti, Fracasso, Franco, Leone, Passarella, Tarter & Velo, 1979). Moreover a sharp rise of copper concentration and caeruloplasmin activity in biological fluids and tissues has been measured in man and animals under a wide variety of acute and chronic inflammatory conditions .…”

Section: Introductionmentioning

confidence: 99%

Copper metabolism during acute inflammation: studies on liver and serum copper concentrations in normal and inflamed rats

Conforti

Franco

Milanino

et al. 1983

British J Pharmacology

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The concentration of copper in serum and liver was determined by atomic absorption spectrophotometry in a study performed on normal rats of either sex and in female rats with carrageenan‐induced pleurisy. In the normal animal, total serum copper concentration is significantly higher in female rats, and appears to be higher in mature animals in females. In normal rats of either sex, liver copper concentration undergoes daily variations which are inversely related to the weight of the organ and which leave constant the total amount of metal in the liver. Moreover a day to day non‐cyclic variability of liver copper concentration and liver weight was observed. This first set of data showed that comparison with time control was essential. In the inflamed rat, a significant rise of total serum copper at 22, 48 and 72 h after the induction of inflammation was observed. From 96 h up to 240 h post‐injection no significant differences were evident. Total liver copper content did not change in the inflamed rats. During acute inflammation in the rat, the copper needed for the increased synthesis of caeruloplasmin is supplied without depletion of liver copper stores.

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“…Furthermore, in two models of acute inflammation (carrageenan-induced foot pad oedema and pleurisy), a copper deficiency was found to exert a pro-inflammatory effect [13]. However, using the 'phenanthroline assay', attempts to detect copper ions in a form which promotes the generation of "OH or 'crypto-'OH' radical species in fresh serum or SF collected from patients with RA have been unsuccessful [14].…”

Section: Introductionmentioning

confidence: 99%

Detection and investigation of the molecular nature of low‐molecular‐mass copper ions in isolated rheumatoid knee‐joint synovial fluid

et al. 1995

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Low-molecular-mass copper(lI) species have been detected and quantified in ultrafiltrates (n = 7) of rheumatoid synovial fluid (SF) by a highly-sensitive HPLC-based assay system with the ability to determine Cu(II) concentrations of < 10 -7 mol.dm -3. High field 1H NMR spectroscopy demonstrated that addition of Cu(II)¢,q.) to isolated samples of RA SF ultrafiltrates resulted in complexation by histidine > alanine > formate > threouine > lactate • tyrosine • phenylalauine, their effectiveness in this context being in the given order. CD spectra of Cu(H)-treated samples of intact SF exhibited absorption bands typical of copper(II)-albumin complexes, in addition to a band attributable to a low-molecular-mass histidinate complex (A~ 610 nm). Since both albumin and histidine are potent radical scavengers, these results indicate that any "OH radical generated from bound copper ions will be 'site-specifically' scavenged. ttence, low-molecular-mass copper complexes with the ability to promote the generation of "OH radical which can then escape trom the metal ion co-ordination sphere (and in turn, cause damage to critical biomolecules) appear to be absent from inflammatory SF.

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Concerning the role of endogenous copper in the acute inflammatory process

Cited by 37 publications

References 27 publications

Copper and ceruloplasmin (Cp) concentrations during the acute inflammatory process in the rat

Copper and ceruloplasmin (Cp) concentrations during the acute inflammatory process in the rat

Copper metabolism during acute inflammation: studies on liver and serum copper concentrations in normal and inflamed rats

Detection and investigation of the molecular nature of low‐molecular‐mass copper ions in isolated rheumatoid knee‐joint synovial fluid

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