The social constructionist perspective has become a useful framework for understanding women’s psychological experiences. However, tools for examining how women construct meanings of their experiences as female in relevant social situations remain limited. Over two studies, our article describes the development and psychometric properties of the Subjective Femininity Stress Scale (SFSS), which assesses women’s stress associated with their subjective experiences of being female. Participants (N = 468) described their personal experiences of what it means to be a woman by completing the sentence “As a woman…” 10 times; participants then rated how often each of the femininity experiences was stressful for them. Participants’ qualitative responses were coded based on 20 dimensions of femininity experiences, and their relationships with SFSS scores were examined. Study 1 provided evidence for test–retest reliability, convergent, and discriminant validity of the SFSS. Study 2 demonstrated concurrent and incremental validity of the SFSS. Several dimensions of femininity experiences were significantly correlated with women’s stress appraisal. The open-ended nature of the SFSS is easy to administer in clinical and organizational settings. The dual qualitative/quantitative aspects of the SFSS enable clients to create personally relevant meanings for their femininity experiences while also providing a stress index for exploring links with health outcomes.