Concepts of Tumor Lectinology

Gabius, Hans‐Joachim

doi:10.3109/07357909709047585

Cited by 91 publications

(58 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The available evidence suggests that this attachment both precedes and is required for tumor cell extravasation and the subsequent invasion of the target organ parenchyma. In search of biochemical epitopes that guide the biologic behavior, attention is increasingly directed to cell surface carbohydrates and their receptors, the endogenous lectins (Fukuda, 1995;Gabius, 1997Gabius, , 2000Listinsky et al, 1998;Muramatsu, 1993). Among them, the selectins belonging to the C-type family serve as a role model for lectins active in cell adhesion (Kansas, 1996;Tedder et al, 1995;Vestweber and Blanks, 1999).…”

Section: Discussionmentioning

confidence: 99%

Binding Sites for Lewis Antigens Are Expressed by Human Colon Cancer Cells and Negatively Affect Their Migration

Hittelet

Camby

Nagy

et al. 2003

Laboratory Investigation

Self Cite

View full text Add to dashboard Cite

SUMMARY:In colon cancer, endothelial cell selectins can promote tumor cell attachment via interactions with sialylated Lewis antigens present at the surface of tumor cells, thereby facilitating tumor cell arrest and transmigration into the extravascular space. However, it is not known whether Lewis antigens interact with colon tumor cells and modify their migration. Our aim was to detect the presence of binding sites on human tumor cells for Lewis a/x antigens and their sialylated derivatives in vitro and in vivo and to analyze their influence on migration of colon cancer cells. The immunocytochemical and histochemical levels of expression of the four Lewis antigens were quantitatively determined in four human colon cancer cell lines and in in vivo nude mice xenografts. The levels of expression of specific binding sites for these sugar epitopes were determined by synthetic neoglycoconjugates. The influence of binding of these carbohydrate ligands on cancer cell migration was quantitatively evaluated by computer-assisted phase-contrast videomicroscopy performed on Matrigel culture supports either left uncoated or coated with neoglycoconjugate presenting synthetic Lewis a , sialyl Lewis a , Lewis x , or sialyl Lewis x antigens. The influence of the calcium concentration in the culture medium on the Lewis antigen-mediated effects was checked. Human colon cancer cells expressed significant amounts of specific binding sites detected by the synthetic probes in addition to the oligosaccharide epitopes. The expression levels differed considerably between the four cell lines and between in vitro and in vivo specimens. Cell migration analysis revealed that the four Lewis antigens markedly decreased the levels of migration of the HCT-15 and LoVo cancer cells. This effect depends on the calcium concentration in the culture medium. Binding sites for Lewis epitopes are present on colon cancer cells. The functional relevance of these sites is indicated by the negative influence on cell migration of a matrix containing the oligosaccharides as ligand parts. (Lab Invest 2003, 83:777-787).

show abstract

Section: Discussionmentioning

confidence: 99%

Binding Sites for Lewis Antigens Are Expressed by Human Colon Cancer Cells and Negatively Affect Their Migration

Hittelet

Camby

Nagy

et al. 2003

Laboratory Investigation

Self Cite

View full text Add to dashboard Cite

show abstract

“…The question of whether native or proteolytically trimmed galectin-3 is active or, if negative, might interfere with galectin-1-mediated effects is thus to be answered. The results of our experiments to infer galectin participation in this growth control system and the extent of functional overlap are expected to have relevance beyond this cell type, because human tumor cells often express more than one galectin (33,34).…”

mentioning

confidence: 94%

Negative Regulation of Neuroblastoma Cell Growth by Carbohydrate-dependent Surface Binding of Galectin-1 and Functional Divergence from Galectin-3

Kopitz¹,

Reitzenstein²,

André³

et al. 2001

Journal of Biological Chemistry

Self Cite

261

192

View full text Add to dashboard Cite

The cell density-dependent growth inhibition of human SK-N-MC neuroblastoma cells is initiated by increased ganglioside sialidase activity leading to elevated cell surface presentation of ganglioside GM1, a ligand of galectin-1. We herein show that the extent of the cell surface expression of the galectin coincides with marked increases of the sialidase activity. Reverse transcriptase-polymerase chain reaction analysis excludes a regulation at the transcriptional level. Exposure of cells to purified galectin-1 reveals its carbohydrate-dependent activity to reduce cell proliferation. Assays to detect DNA fragmentation biochemically and cytometrically and to block caspases render it unlikely that galectin-1 acts as a classical proapoptotic factor on these cells. Because the chimeric galectin-3 shares binding sites and binding parameters with galectin-1 for these cells, we tested whether this galectin will elicit the same response as the homodimeric cross-linking galectin-1. Evidently, galectin-3 fails to affect cell growth by itself but interferes with galectin-1 upon coincubation. Its proteolytically truncated variant, the C-terminal lectin domain with impaired capacity to form aggregates when surface bound, has only weak binding properties. Thus, the way in which the galectin-1 interacts topologically with an apparently common set of ligands relative to galectin-3 is crucial for eliciting post-binding events. We conclude that galectin-1 is a probable effector in the sialidase-dependent growth control in this system. Moreover, the experiments with galectin-3 reveal functional divergence, most probably based on different topologies of presentation of homologous carbohydratebinding sites.

show abstract

“…In this context, the analysis of protein-carbohydrate interactions is receiving increasing attention. Because it is known that malignancy is associated with characteristic changes in glycosylation, the ensuing alterations in this system (sugar coding) can well have a bearing on establishing the tumor phenotype (15)(16)(17)(18)(19). One family of proteins of interest in this respect are the galectins, an evolutionarily ancient group of lectins that are found in species ranging from sponges and nematodes to humans (20 -24).…”

mentioning

confidence: 99%

Prognostic Values of Galectin-3 and the Macrophage Migration Inhibitory Factor (MIF) in Human Colorectal Cancers

et al. 2003

Self Cite

View full text Add to dashboard Cite

show abstract

Concepts of Tumor Lectinology

Cited by 91 publications

References 104 publications

Binding Sites for Lewis Antigens Are Expressed by Human Colon Cancer Cells and Negatively Affect Their Migration

Binding Sites for Lewis Antigens Are Expressed by Human Colon Cancer Cells and Negatively Affect Their Migration

Negative Regulation of Neuroblastoma Cell Growth by Carbohydrate-dependent Surface Binding of Galectin-1 and Functional Divergence from Galectin-3

Prognostic Values of Galectin-3 and the Macrophage Migration Inhibitory Factor (MIF) in Human Colorectal Cancers

Contact Info

Product

Resources

About