Concept for Improved Handling Ensures Effective Contactless Plasma Treatment of Patients with kINPen® MED

Hahn, Veronika; Grollmisch, Daniel; Bendt, Hannes; Woedtke, Thomas von; Nestler, B.; Weltmann, Klaus‐Dieter; Gerling, Torsten

doi:10.3390/app10176133

Cited by 8 publications

(2 citation statements)

References 40 publications

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“…Gas sensors to measure these reactive species in the ambient environment can be added to the system in a similar manner to that of DHT22. To improve the handling of the plasma source device operation, the future iteration will also consider implementing concept methods as described in [ 31 , 32 ]. With the integration of the mentioned features, MSD might provide a complete solution for plasma wound treatment documentation.…”

Section: Discussionmentioning

confidence: 99%

Development of a Mobile Sensory Device to Trace Treatment Conditions for Various Medical Plasma Source Devices

Siffa

Gerling²,

Masur³

et al. 2022

Sensors

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The emerging use of low-temperature plasma in medicine, especially in wound treatment, calls for a better way of documenting the treatment parameters. This paper describes the development of a mobile sensory device (referred to as MSD) that can be used during the treatment to ease the documentation of important parameters in a streamlined process. These parameters include the patient’s general information, plasma source device used in the treatment, plasma treatment time, ambient humidity and temperature. MSD was developed as a standalone Raspberry Pi-based version and attachable module version for laptops and tablets. Both versions feature a user-friendly GUI, temperature–humidity sensor, microphone, treatment report generation and export. For the logging of plasma treatment time, a sound-based plasma detection system was developed, initially for three medically certified plasma source devices: kINPen® MED, plasma care®, and PlasmaDerm® Flex. Experimental validation of the developed detection system shows accurate and reliable detection is achievable at 5 cm measurement distance in quiet and noisy environments for all devices. All in all, the developed tool is a first step to a more automated, integrated, and streamlined approach of plasma treatment documentation that can help prevent user variability.

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Section: Discussionmentioning

confidence: 99%

Development of a Mobile Sensory Device to Trace Treatment Conditions for Various Medical Plasma Source Devices

Siffa

Gerling²,

Masur³

et al. 2022

Sensors

Self Cite

View full text Add to dashboard Cite

show abstract

“…This was found mainly by adding antioxidant molecules or enzymes, such as N-acetylcysteine and catalase, to the samples, finding that many of the gas plasma-derived effects were reversed. However, only a few have convincingly shown how to optimize antimicrobial effects of existing gas plasma devices [ 19 , 20 ]. With many device configurations, especially gas plasma jets, the resulting ROS mixture expelled depends on the feed gas composition.…”

Section: Introductionmentioning

confidence: 99%

Argon Humidification Exacerbates Antimicrobial and Anti-MRSA kINPen Plasma Activity

Clemen

Singer

Skowski

et al. 2023

Life

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Gas plasma is a medical technology with antimicrobial properties. Its main mode of action is oxidative damage via reactive species production. The clinical efficacy of gas plasma-reduced bacterial burden has been shown to be hampered in some cases. Since the reactive species profile produced by gas plasma jets, such as the kINPen used in this study, are thought to determine antimicrobial efficacy, we screened an array of feed gas settings in different types of bacteria. Antimicrobial analysis was performed by single-cell analysis using flow cytometry. We identified humidified feed gas to mediate significantly greater toxicity compared to dry argon and many other gas plasma conditions. The results were confirmed by inhibition zone analysis on gas-plasma-treated microbial lawns grown on agar plates. Our results may have vital implications for clinical wound management and potentially enhance antimicrobial efficacy of medical gas plasma therapy in patient treatment.

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Proteomic profiling of antibiotic‐resistant Escherichia coli GW‐AmxH19 isolated from hospital wastewater treated with physical plasma

Hahn,

Zühlke,

Winter

et al. 2024

Proteomics

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Microorganisms which are resistant to antibiotics are a global threat to the health of humans and animals. Wastewater treatment plants are known hotspots for the dissemination of antibiotic resistances. Therefore, novel methods for the inactivation of pathogens, and in particular antibiotic‐resistant microorganisms (ARM), are of increasing interest. An especially promising method could be a water treatment by physical plasma which provides charged particles, electric fields, UV‐radiation, and reactive species. The latter are foremost responsible for the antimicrobial properties of plasma. Thus, with plasma it might be possible to reduce the amount of ARM and to establish this technology as additional treatment stage for wastewater remediation. However, the impact of plasma on microorganisms beyond a mere inactivation was analyzed in more detail by a proteomic approach. Therefore, Escherichia coli GW‐AmxH19, isolated from hospital wastewater in Germany, was used. The bacterial solution was treated by a plasma discharge ignited between each of four pins and the liquid surface. The growth of E. coli and the pH‐value decreased during plasma treatment in comparison with the untreated control. Proteome and antibiotic resistance profile were analyzed. Concentrations of nitrite and nitrate were determined as long‐lived indicative products of a transient chemistry associated with reactive nitrogen species (RNS). Conversely, hydrogen peroxide served as indicator for reactive oxygen species (ROS). Proteome analyses revealed an oxidative stress response as a result of plasma‐generated RNS and ROS as well as a pH‐balancing reaction as key responses to plasma treatment. Both, the generation of reactive species and a decreased pH‐value is characteristic for plasma‐treated solutions. The plasma‐mediated changes of the proteome are discussed also in comparison with the Gram‐positive bacterium Bacillus subtilis. Furthermore, no effect of the plasma treatment, on the antibiotic resistance of E. coli, was determined under the chosen conditions. The knowledge about the physiological changes of ARM in response to plasma is of fundamental interest to understand the molecular basis for the inactivation. This will be important for the further development and implementation of plasma in wastewater remediation.

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Concept for Improved Handling Ensures Effective Contactless Plasma Treatment of Patients with kINPen® MED

Cited by 8 publications

References 40 publications

Development of a Mobile Sensory Device to Trace Treatment Conditions for Various Medical Plasma Source Devices

Development of a Mobile Sensory Device to Trace Treatment Conditions for Various Medical Plasma Source Devices

Argon Humidification Exacerbates Antimicrobial and Anti-MRSA kINPen Plasma Activity

Proteomic profiling of antibiotic‐resistant Escherichia coli GW‐AmxH19 isolated from hospital wastewater treated with physical plasma

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