Concentrations of Seven Elements in the Intraluminal Fluids of the Rat Seminiferous Tubules, Rete Testis, and Epididymis1

Jenkins, Alan D.; Lechène, C.; Howards, Stuart S.

doi:10.1095/biolreprod23.5.981

Cited by 82 publications

(62 citation statements)

References 11 publications

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“…These changes occur in the male (epididymal maturation) and female reproductive tracts (capacitation and AR), and involve important variations in the ionic composition of the external media (reviewed in [6,43,44]). Increases in extracellular [K +] known to occur during the sperm's journey [45], could depolarize them and open voltage-dependent Ca z+ channels triggering premature exocytosis. Having T-type Ca z+ channels in uncapacitated sperm, which would be largely inactivated at their estimated resting membrane potential (around -40 mV; [46,47]), instead of L-type which would not be as inactivated, could therefore be a safeguard to contend with the environmental changes that occur during its maturation.…”

Section: Resultsmentioning

confidence: 99%

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Liévano¹,

Santi

Serrano³

et al. 1996

FEBS Letters

160

153

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There is pharmacological evidence that Ca 2+ channels play an essential role in triggering the mammalian sperm acrosome reaction, an exocytotic process required for sperm to fertilize the egg. Spermatozoa are small terminally differentiated cells that are difficult to study by conventional electrophysiological techniques. To identify the members of the voltagedependent Ca z+ channel family possibly present in sperm, we have looked for the expression of the a~A, tXlB, ale, atD and a~E genes in mouse testis and in purified spermatogenic cell populations with RT-PCR. Our results indicate that all 5 genes are expressed in mouse testis, and in contrast only alE, and to a minor extent CqA, are expressed in spermatogenic cells. In agreement with these findings, only T-type Ca 2+ channels sensitive to the dihydropyridine nifedipine were observed in patch-clamp recordings of pachytene s permatocytes. These 2+ results suggest that low-threshold Ca channels are the dihydropyridine-sensitive channels involved in the sperm acrosome reaction.

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Section: Resultsmentioning

confidence: 99%

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Liévano¹,

Santi

Serrano³

et al. 1996

FEBS Letters

160

153

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“…This is caused by differences in secretory and absorptive activities of the epididymal epithelium (12)(13)(14)(15)(16), resulting in changes of the net water; the HCO 3 Ϫ reabsorption; the secretion of proteins; concentrations of Na ϩ , Cl Ϫ , and K ϩ ; and luminal acidification. Notably, the Ca 2ϩ concentration decreases markedly in the epididymal fluid along the epididymal duct toward its distal segments, the cauda epididymis (14). It is this luminal Ca 2ϩ gradient that is severely compromised by the TRPV6 D541A mutation, resulting in a dramatic but incomplete loss of sperm motility and fertilization capacity.…”

Section: Trpv6mentioning

confidence: 99%

Excision of Trpv6 Gene Leads to Severe Defects in Epididymal Ca2+ Absorption and Male Fertility Much Like Single D541A Pore Mutation

Weißgerber

Kriebs

Tsvilovskyy

et al. 2012

Journal of Biological Chemistry

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Background: The TRPV6D541A pore mutation abrogates epididymal Ca 2ϩ absorption causing hypofertility in mice, raising the possibility of residual TRPV6 D541A channel activity. Results: Trpv6 deletion reduces fertility parameters to the same extent as the D541A pore mutation. Conclusion:The D541A pore mutation leads to complete inactivation of TRPV6 channels in epididymal epithelium. Significance: Targeted mutations in mice help to understand the function of TRPV6 proteins in native systems.

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“…This discrepancy may be attributed to other transporters or regional differences of intrinsic carnitine or inorganic ions (Na C or K C , etc.) inside and outside of spermatozoa (Levine & Marsh 1971, Turner et al 1977, Jenkins et al 1980, Jeulin & Lewin 1996.…”

Section: Carnitine Transport In Spermatozoamentioning

confidence: 99%

Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa

Kobayashi¹,

Tamai²,

Sai³

et al. 2007

Reproduction

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Carnitine and acetylcarnitine are important for the acquisition of motility and maturation of spermatozoa in the epididymis. In this study, we examined the involvement of carnitine/organic cation transporter (OCTN) in carnitine and acetylcarnitine transport in epididymal spermatozoa of mice. Uptake of both compounds by epididymal spermatozoa was time-dependent and partially Na C -dependent. Kinetic analyses revealed the presence of a high-affinity transport system in the spermatozoa, with K m values of 23.6 and 6.57 mM for carnitine and acetylcarnitine respectively in the presence of Na C . Expression of OCTN2 and OCTN3 in epididymal spermatozoa was confirmed by immunofluorescence analysis. The involvement of these two transporters in carnitine and acetylcarnitine transport was supported by a selective inhibition study. We conclude that both Na C -dependent and -independent carnitine transporters, OCTN2 and OCTN3, mediate the supply of carnitine and acetylcarnitine to epididymal spermatozoa in mice.

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Concentrations of Seven Elements in the Intraluminal Fluids of the Rat Seminiferous Tubules, Rete Testis, and Epididymis1

Cited by 82 publications

References 11 publications

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Excision of Trpv6 Gene Leads to Severe Defects in Epididymal Ca2+ Absorption and Male Fertility Much Like Single D541A Pore Mutation

Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa

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Concentrations of Seven Elements in the Intraluminal Fluids of the Rat Seminiferous Tubules, Rete Testis, and Epididymis1

Cited by 82 publications

References 11 publications

T‐type Ca2+ channels and α1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca2+ channels and α1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

Excision of Trpv6 Gene Leads to Severe Defects in Epididymal Ca2+ Absorption and Male Fertility Much Like Single D541A Pore Mutation

Transport of carnitine and acetylcarnitine by carnitine/organic cation transporter (OCTN) 2 and OCTN3 into epididymal spermatozoa

Contact Info

Product

Resources

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T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction

T‐type Ca²⁺ channels and α_1E expression in spermatogenic cells, and their possible relevance to the sperm acrosome reaction