Concentrations of Myeloperoxidase in Nasal Secretions of Atopic Patients after Nasal Allergen Challenge and during Natural Allergen Exposure

Wang, Deyun; Smitz, Johan; Derde, Marie-Paule; Clement, Péter

doi:10.1159/000237316

Cited by 17 publications

(6 citation statements)

References 19 publications

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“…Recently, Wang et al [37] used a microsuction technique to measure the concentration of MPO in nasal secretions. Using this technique, the authors were not able to demonstrate any LP increase in MPO levels following nasal allergen challenge of allergic patients.…”

Section: Discussionmentioning

confidence: 99%

IL-8 and the Activation of Eosinophils and Neutrophils following Nasal Allergen Challenge

Jacobi

Poulsen

Reimert

et al. 1998

Int Arch Allergy Immunol

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Background: A growing body of evidence suggests that proinflammatory cytokines play a role in allergic inflammation by attracting and activating inflammatory cells. In this study, we have investigated the relationship between interleukin-8 (IL-8) in nasal lavage fluid and the local activation of eosinophils and neutrophils following nasal allergen challenge of allergic patients. Methods: Nasal challenges were performed with grass pollen extract in 14 allergic patients and 5 nonallergic controls. Nasal lavage fluid was collected repeatedly for 10 h, and the levels of eosinophil cationic protein (ECP) and myeloperoxidase (MPO) were used as markers of eosinophil and neutrophil activation, respectively. The levels of these molecules were compared with that of IL-8 in nasal lavage fluid. Results: Allergen challenge of allergic patients produced a significant late-phase increase in the levels of ECP and MPO. Furthermore, the level of MPO showed a highly significant correlation with the level of IL-8 in lavage fluid (r = 0.8, p < 0.0001), whereas there was no significant relationship between the levels of ECP and IL-8. Conclusion: Interestingly, our findings suggest that both eosinophils and neutrophils are activated following nasal allergen challenge. In addition, our results are consistent with the hypothesis that IL-8 acts as a chemoattractant/activator of neutrophils during the late phase of the allergic inflammation. In contrast, we were not able to demonstrate any significant relationship between the level of IL-8 in lavage fluid and the activation of eosinophils.

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Section: Discussionmentioning

confidence: 99%

IL-8 and the Activation of Eosinophils and Neutrophils following Nasal Allergen Challenge

Jacobi

Poulsen

Reimert

et al. 1998

Int Arch Allergy Immunol

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“…In the clinical setting, it is often used to diagnose nasal obstruction and to follow patients treated with medical and surgical procedures to improve nasal patency [9, 10]. Moreover, it can be useful for observing seasonal allergic rhinitis [11], studying nasal hyperresponsiveness [12–14], evaluating various intranasal treatments [15, 16], and for the follow-up of nasal provocation testing with allergic response mediators [17, 18]. The measurement of unilateral nasal resistance also detects anatomical obstructions and helps evaluate the efficacy of corrective surgery.…”

Section: Introductionmentioning

confidence: 99%

Nasal Airflow Measured by Rhinomanometry Correlates with FeNO in Children with Asthma

et al. 2016

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BackgroundRhinitis and asthma share similar immunopathological features. Rhinomanometry is an important test used to assess nasal function and spirometry is an important tool used in asthmatic children. The degree to which the readouts of these tests are correlated has yet to be established. We sought to clarify the relationship between rhinomanometry measurements, fractional exhaled nitric oxide (FeNO), and spirometric measurements in asthmatic children.MethodsPatients’ inclusion criteria: age between 5 and 18 years, history of asthma with nasal symptoms, and no anatomical deformities. All participants underwent rhinomanometric evaluations and pulmonary function and FeNO tests.ResultsTotal 84 children were enrolled. By rhinomanometry, the degree of nasal obstruction was characterized as follows: (1) no obstruction in 33 children, (2) slight obstruction in 29 children, and (3) moderate obstruction in 22 children. FeNO was significantly lower in patients without obstruction than those with slight or moderate obstruction. Dividing patients according to ATS Clinical Practice Guidelines regarding FeNO, patients < 12 years with FeNO > 20 ppb had a lower total nasal airflow rate than those with FeNO < 20 ppb. Patients ≥ 12 years with FeNO > 25 ppb had a lower total nasal airflow rate than those with FeNO < 25 ppb.ConclusionsHigher FeNO was associated with a lower nasal airflow and higher nasal resistance. This supports a relationship between upper and lower airway inflammation, as assessed by rhinomanometry and FeNO. The results suggest that rhinomanometry may be integrated as part of the functional assessment of asthma.

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“…Primary outcomes were levels of interleukin-5 (IL-5), IL-8, eosinophil cationic protein (ECP), myeloperoxidase (MPO) and cell differentials obtained during the phase of the late allergic reaction (4–24 h). Early protein leakage (α 2 -macroglobulin, A2M) and histamine levels were monitored as an indicator for early mast cell degranulation in the nose [12, 13, 14, 15]. The study was designed to detect a 25% difference in late skin reactivity and a 30% difference in log-normalized levels of soluble markers with a power of 0.80 and a 95% confidence level.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Allergen-Induced Late Inflammatory Reactions in the Nose and in the Skin in House Dust Mite-Allergic Patients with or without Asthma

Lopuhaä

Riemer²,

Out³

et al. 2003

Int Arch Allergy Immunol

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Background: It remains to be established which factors contribute to the occurrence of asthma in allergic individuals. We hypothesized that differences in the late allergic inflammatory reaction to allergen between asthmatic and non-asthmatic house dust mite-allergic individuals might contribute to the difference in the clinical presentation of allergy. Aim: To compare allergen-induced changes in parameters for cellular inflammation during the phase of the late allergic reaction in the skin and nose, in house dust mite-allergic individuals with or without asthma. Material and Methods: Nasal and dermal allergen challenges with house dust mite (Dermatophagoides pteronyssinus) extract were performed in 52 house dust mite-allergic individuals, of whom 26 had mild to moderate persistent asthma and 26 had perennial rhinitis without current or past asthmatic symptoms. Serial nasal lavage samples were analyzed for the presence of inflammatory cells (eosinophils and neutrophils) and soluble markers associated with cellular inflammation [interleukin-5 (IL-5), interleukin-8 (IL-8), eosinophil cationic protein (ECP) and myeloperoxidase (MPO)]. Macroscopic late phase skin reactions were studied after intracutaneous skin tests with house dust mite extract. Results: Fixed dose nasal allergen provocation elicited a similar degree of immediate allergic reaction as judged by plasma protein exudation and histamine concentrations in asthma and non-asthmatic rhinitis. Subsequently, no differences between groups were found during the phase of the late allergic reaction (4–24 h) in inflammatory cell influx, plasma protein leakage, ECP or MPO. Likewise, there were no differences in levels of chemotactic cytokines IL-5 and IL-8. In agreement with the results of nasal challenge, the late skin reaction after dermal challenge with a fixed allergen dose and after an allergen dose 10,000 times above the skin threshold for an early skin reaction did not differ between the groups. Conclusion: House dust mite-allergic patients with or without asthma have very similar late allergic inflammatory reactions in the skin and in the nose after allergen challenge. Hence, it is unlikely that the occurrence of pulmonary symptoms in asthma is explained by a general tendency of asthmatics to have an enhanced late allergic cellular inflammatory response. Nasal and dermal allergen provocations are adequate models to study allergen-induced inflammation but probably lack the pivotal link which is essential for the development of asthma.

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Concentrations of Myeloperoxidase in Nasal Secretions of Atopic Patients after Nasal Allergen Challenge and during Natural Allergen Exposure

Cited by 17 publications

References 19 publications

IL-8 and the Activation of Eosinophils and Neutrophils following Nasal Allergen Challenge

IL-8 and the Activation of Eosinophils and Neutrophils following Nasal Allergen Challenge

Nasal Airflow Measured by Rhinomanometry Correlates with FeNO in Children with Asthma

Comparison of Allergen-Induced Late Inflammatory Reactions in the Nose and in the Skin in House Dust Mite-Allergic Patients with or without Asthma

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