Concentrations of growth factors in platelet-rich plasma and platelet-rich fibrin in a rabbit model

Abstract

Platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are platelet concentrates which have been used to improve healing and regeneration of damaged tissues. The concentration of growth factors greatly influences the final outcome of the treatment. For studying the effects of PRP and PRF, an animal model is required. The New Zealand White rabbit appears to be a good candidate for studying the features of these blood derivatives. This article presents for the first time the various preparation methods of blood derivatives with regard to concentration of growth factors -insulin-like growth factor, transforming growth factor and vascular endothelial growth factor. There was no statistically significant difference among the methods in the case of the first two factors. In the case of vascular endothelial growth factor, the lowest amount was detected in PRF. This study, even though performed on a limited number of animals, reports novel characterisation of the rabbit as an animal model for in vivo studies of the action of these blood derivatives.

Platelet rich plasma and amnion-derived fluid as clinical options for regenerative medicine applications

“…They include platelet derived growth factor (PDGF), transforming growth factor beta (TGF-β), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) [5,6,9]. TGF-β is an immunoregulatory cytokine that contributes to the suppression of the inflammatory response, VEGF is a growth factor that is associated with increasing vascular permeability, angiogenesis, and also has antiapoptotic functions [3]. PDGF, bFGF, and EGF are cellular mitogens that promote growth in a wide array of cell types, and serve an important role in the regenerative process associated with PRP [10].…”

“…While regenerative medicine therapies have become more popular in recent times, the specific mechanisms of healing are still being explored. The current hypothesis is that the major therapeutic benefits of regenerative medicine treatments come from the paracrine action of trophic factors contained in significant concentrations which signal, among many things, the endogenous progenitor cells to begin proliferation and healing [1,2,3].…”

Advance-Platelet Rich Fibrin and Hyaluronic Acid Combination Improves Interleukin-6 and Granulation Index in Diabetic Foot Ulcer Patients

“…(11) Some studies showed that combining HA with PRP exhibits anti-inflammatory properties in subjects with knee osteoarthritis. (12) In patients with DFU, the process of wound healing is delayed due to prolonged inflammation, and inhibit growth factors to form granulation tissue in the proliferation and epithelialization phases needed for wound healing. ( 13) Both HA and A-PRF have an anti-inflammatory property.…”

Human platelet-rich plasma as a biological stimulant for proliferation and differentiation of mesenchymal stem cells

“…These growth factors play important roles in promoting tissue regeneration and repair. PRP must be activated to release various growth factors and regulatory proteins, such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor-beta (TGF-ß), insulin-like growth factors (IGFs), and vascular endothelial growth factor (VEGF) (14,17,18). These growth factors are known to play crucial roles in stem cell physiology, such as proliferation, migration, and differentiation.…”