Concentration of stromal cell‐derived factor‐1 (SDF‐1/CXCL12) in the follicular fluid is associated with blastocyst development

Abstract

Purpose To study the association between stromal cell‐derived factor‐1 (SDF‐1/CXCL12) and vascular endothelial growth factor (VEGF) concentrations in individual human ovarian follicles and IVF outcomes. Methods Concentrations of SDF‐1 and VEGF in 261 follicular fluid samples were measured with enzyme‐linked immunosorbent assay. IVF outcome parameters were included in fertilization rate, cleavage rate, embryo morphology on day 3, and blastocyst morphology on day 5. Results The follicular concentration of SDF‐1 and VEGF was not significantly associated with fertilization and cleavage outcome, and embryo morphology. The rates of full blastocysts and good‐quality blastocysts were significantly higher in follicles with an SDF‐1 concentration of 275‐350 pg/mL than in the follicles with SDF‐1 concentrations of <200 and ≥350 pg/mL ( P < 0.05). The follicular concentration of VEGF was not associated with the blastocyst morphology. Conclusion Our findings showed that follicular concentration of SDF‐1, and not VEGF, may be a valuable biochemical marker of blastocyst development.

Supplementation of SDF1 during Pig Oocyte In Vitro Maturation Improves Subsequent Embryo Development

“…Human leukemia cells cultured in the presence of SDF1 produce low levels of ROS due to lower mitochondrial metabolism [ 24 ]. The level of SDF1 in human [ 25 ] and porcine follicular fluid [ 22 ] is predictive of the outcome of embryonic development. Previous studies have shown that supplementation of SDF1 alone during ovine oocyte IVM can enhance subsequent PA embryo development, and combined supplementation of SDF1 with other factors to porcine oocyte IVM medium can improve subsequent embryo development [ 26 , 27 ].…”

SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the JAK2/STAT3 pathway

“…BMSCs have the potential for cross-system and cross-germ differentiation under the action of a specific microenvironment and suitable cytokines/growth factors. They can differentiate into mesodermal and neuroectodermal cells, such as osteoblasts, chondrocytes, endothelial cells, and nerve cells; accordingly, they have recently become a resource for tissue repair [1,2]. Osteogenic differentiation of BMSCs is closely related to bone-related diseases: for example, the inhibition of osteoporosis [3].…”