“…The values for the fresh samples are discontinuous at the limit of hydraulic pressure and are joined by dotted lines to values obtained on the frozen and re-expressed press-cakes. The approximately constant values for the different fractions of sap from frozen tissue up to a removal of 90 per cent of the total liquid present indicate that the expressed liquid was representative of the tissue as a whole, associated with the destruction of the normal state of the living substance (Lepeschkin, 1936;Mason and Phillis, 1936;Phillis and Mason, 1937). Freezing, like heat killing, cytolysis, and thorough grinding, apart from other possible objections, has made possible the release of the tissue fluids in a composite mixture.…”