Concentration of Selected Metalloproteinases and Osteocalcin in the Serum and Synovial Fluid of Obese Women with Advanced Knee Osteoarthritis

Abstract

The aim of the study was to evaluate the levels of selected MMPs (matrix metalloproteinases) and osteocalcin in the serum and synovial fluid of obese women with osteoarthritis and their correlations with clinical status. The studied group consisted of 39 overweight females undergoing primary total knee arthroplasty due to osteoarthritis (OA). The staging of knee OA was evaluated according to the Ahlbӓck and Kellgren–Lawrence scores. Synovial fluid and peripheral blood samples were obtained. The levels of selected MMPs and osteocalcin were assessed using commercial ELISA kits. The mean value of MMP3 was significantly higher in patients with more advanced disease in both serum (p = 0.0067) and synovial fluid (p = 0.0328). The pro-MMP13 level tended to be higher in synovial fluid in the case of more advanced stages (p = 0.0882), with no tendency regarding the serum level (p = 0.9595). The synovial level of pro-MMP1 was significantly correlated with the synovial concentration of MMP9 and MMP3. The synovial level of MMP9 also showed a significant correlation with the synovial level of MMP3 and pro-MMP13. Furthermore, it was found that the serum level of MMP3 was significantly correlated with the synovial pro-MMP13 level. A correlation between the osteocalcin level in serum and its synovial level was determined. The serum MMP9 level showed a significant correlation with BMI, whereas the synovial MMP9 level was notably correlated with age. Our results showed that the levels of MMP3, MMP9, and pro-MMP13 increased in more advanced radiological stages of OA, indicating the underlying inflammatory process of OA.

Diagnostic value of matrix metalloproteinase-9 in the course of osteoarthritis in young persons with excessive body weight and obesity

“…and Jarecki, J. et al . recognized regular growth levels of MMP-3, MMP-9 and pro-MMP-13 when changing X-ray stage damage joints that explain active inflammatory process in OA [16,17]. We are too confirmed specified pattern , which, in our opinion, is not only the result of inflammation reaction , but also progressive degradation cartilage fabrics.…”

“…associated with the arrival of leptin from apodocytes , which they considered as the key mediator that modulates degrading functions chondrocytes through regulation MMP -9 and MMP-13 [17].…”

Assessment of the Impact of Physical Activity on the Musculoskeletal System in Early Degenerative Knee Joint Lesions in an Animal Model

“…At the early OA stages, the Hoffa fat pad (IPFP—infrapatellar fat pad) increases its activity. The exact mechanism of the development of these changes in early OA is not clear but may consist in increased production of cytokines, adipokines, interleukins, and growth factors secreted by IPFP, which accelerate cartilage destruction through enhanced production of matrix metalloproteinases [ 1 , 61 , 62 ]. There are also no data showing changes in the IPFP volume in early OA.…”

Elucidation of the Key Therapeutic Targets and Potential Mechanisms of Marmesine against Knee Osteoarthritis via Network Pharmacological Analysis and Molecular Docking

“…Matrix metallopeptidase 9 (MMP9) has been involved in the pathological process of various diseases, including OA. The increased expression of MMP9 promoted the progression of diabetic OA via accelerating chondrocyte apoptosis and suppressing cartilage differentiation [ 33 , 34 ]. MMP9 was upregulated in synovial fluid of patients with OA [ 35 ], and increased MMP9 protein level may be related to the pathogenesis of OA [ 36 ].…”