Concentration gradient generation methods based on microfluidic systems

Wang, Xiang; Liu, Zhaomiao; Pang, Yan

doi:10.1039/c7ra04494a

Cited by 172 publications

(120 citation statements)

References 196 publications

(236 reference statements)

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“…Gradient generation by CGG provided five different irinotecan concentration conditions and was suited for HTS with a large number of spheroids in parallel. The concentrations can be also easily calculated [34,35]. Thus, the irinotecan concentrations at the channels (C1-C5) are calculated to be 0, 1.25, 2.5, 3.75 and 5 μM, respectively.…”

Section: Discussionmentioning

confidence: 99%

A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Lim¹,

Park²

2018

Preprint

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Three-dimensional (3D) cell culture is considered more clinically relevant in mimicking the structural and physiological conditions of tumors in vivo compared to two-dimensional cell cultures. In recent years, high-throughput screening (HTS) in 3D cell arrays has been extensively used for drug discovery because of its usability and applicability. Herein, we developed a microfluidic spheroid culture device (μFSCD) with a concentration gradient generator (CGG) that enabled cells to form spheroids and grow in the presence of cancer drug gradients. The device is composed of concave microwells with several serpentine micro-channels which generate a concentration gradient. Once the colon cancer cells (HCT116) formed a single spheroid (approximately 120 μm in diameter) in each microwell, spheroids were perfused in the presence of the cancer drug gradient irinotecan for 3 days. The number of spheroids, roundness, and cell viability, were inversely proportional to the drug concentration. These results suggest that the μFSCD with a CGG has the potential to become an HTS platform for screening the efficacy of cancer drugs.

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Section: Discussionmentioning

confidence: 99%

A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Lim¹,

Park²

2018

Preprint

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“…For static differences within environments created by microfluidic gradient generators the reader is referred to recent reviews summarizing the field of microfluidic gradient generators. [ 54,55 ] Afterward, the cultivation chamber is introduced in context of a rapid DCE and an overview of the different combination of technical periphery and cultivation chambers is shortly discussed.…”

Section: Microfluidic Setups For Dynamically Controlled Environments mentioning

confidence: 99%

Dynamic Environmental Control in Microfluidic Single‐Cell Cultivations: From Concepts to Applications

Täuber

Lieres

Grünberger

2020

Small

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Microfluidic single‐cell cultivation (MSCC) is an emerging field within fundamental as well as applied biology. During the last years, most MSCCs were performed at constant environmental conditions. Recently, MSCC at oscillating and dynamic environmental conditions has started to gain significant interest in the research community for the investigation of cellular behavior. Herein, an overview of this topic is given and microfluidic concepts that enable oscillating and dynamic control of environmental conditions with a focus on medium conditions are discussed, and their application in single‐cell research for the cultivation of both mammalian and microbial cell systems is demonstrated. Furthermore, perspectives for performing MSCC at complex dynamic environmental profiles of single parameters and multiparameters (e.g., pH and O2) in amplitude and time are discussed. The technical progress in this field provides completely new experimental approaches and lays the foundation for systematic analysis of cellular metabolism at fluctuating environments.

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“…Microfluidics are divided into four groups including 1) convection, 2) laminar flow diffusion, 3) static diffusion, and 4) geometric metering with respect to their principles of gradient generation 55–58. Microfluidic gradient devices are usually portable, inexpensive, quick gradient device, require low power, and enable to manipulate fluidic volume with experimentally reproducible 59–62. Many researchers choose MD as an effective gradient‐forming approach due to its diverse advantages indeed.…”

Section: Microfluidic Devicementioning

confidence: 99%

“…to manipulate fluidic volume with experimentally reproducible. [59][60][61][62] Many researchers choose MD as an effective gradient-forming approach due to its diverse advantages indeed. The microfluidic gradient-generator device generally has four parts, including 1) inlet, 2) outlet, 3) gradient chamber and 4) mixing channels.…”

Section: Microfluidic Devicementioning

confidence: 99%

Recent Strategies in Fabrication of Gradient Hydrogels for Tissue Engineering Applications

Yoon

Gajendiran

et al. 2019

Macromolecular Bioscience

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Hydrogels are widely used as scaffold in tissue engineering field because of their ability to mimic the cellular microenvironment. However, mimicking a completely natural cellular environment is complicated due to the differences in various physical and chemical properties of cellular environments. Recently, gradient hydrogels provide excellent heterogeneous environment to mimic the different cellular microenvironments. To create hydrogels with an anisotropic distribution, gradient hydrogels have been widely developed by adopting several gradient generation techniques. Herein, the various gradient hydrogel fabrication techniques, including dual syringe pump systems, microfluidic device, photolithography, diffusion, and bio‐printing are summarized. As the effects of gradient 3D hydrogels with stems have been reviewed elsewhere, this review focuses principally on gradient hydrogel fabrication for multi‐model tissue regeneration. This review provides new insights into the key points for fabrication of gradient hydrogels for multi‐model tissue regeneration.

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Concentration gradient generation methods based on microfluidic systems

Abstract: Various concentration gradient generation methods based on microfluidic systems are summarized in this paper.

Cited by 172 publications

References 196 publications

A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

A Microfluidic Spheroid Culture Device with a Concentration Gradient Generator for High-Throughput Screening of Drug Efficacy

Dynamic Environmental Control in Microfluidic Single‐Cell Cultivations: From Concepts to Applications

Recent Strategies in Fabrication of Gradient Hydrogels for Tissue Engineering Applications

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