Concanavalin A receptors on the surface membrane of lymphocytes from patient's with Hodgkin's disease and other malignant lymphomas.

Ben‐Bassat, Hannah; Goldblum, Natan

doi:10.1073/pnas.72.3.1046

Cited by 36 publications

(16 citation statements)

References 17 publications

(8 reference statements)

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“…We have previously shown that lymphocytes from patients with chronic lymphocytic leukemia, patients with Hodgkin's disease and other malignant lymphomas have a reduced mobility of Con A sites Goldblum, 1974, 1975). This is true for lymphocytes isolated from biopsy material of the patients, as well as for lymphocytes isolated from peripheral blood (Ben-Bassat and Goldblum, 1975 ;Ben-Bassat et al, 1976). Lymphocytes isolated from peripheral blood of patients with carcinoma exhibited a mobility of Con A sites similar to that of normal lymphocytes.…”

Section: Discussionmentioning

confidence: 86%

“…A significant increase in agglutin- ability is evident for lymphocytes from patients with Burkitt's lymphoma as compared to healthy donors and patients with carcinoma. We have previously shown that B and T blood lymphocytes from normal healthy persons are agglutinated by Con A to the same extent (Ben-Bassat and Goldblum, 1975). Also, in these cases the agglutinability of lymphocytes from patients with Burkitt's lymphoma was found not to be due to changes ifi the relative proportions of B-and T-lymphocytes in the blood.…”

Section: Lymphomamentioning

confidence: 92%

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Concanavalin a receptors on the surface membrane of lymphocytes from patients with african Burkitt's lymphoma and lymphoma cell lines

Ben‐Bassat

Goldblum

Mitrani

et al. 1976

Intl Journal of Cancer

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Lymphocytes isolated from the peripheral blood and from tumor tissues of patients with African Burkitt's lymphoma have been studied for cap formation and agglutinability by Concanavalin A (Con A). Peripheral blood from healthy adult persons served as a normal control and blood from patients with carcinoma served as a non-lymphoma control. These studies included 29 patients with Burkitt's lymphoma, 93 with carcinoma, and 105 healthy adult persons, as well as tumor tissues from 13 patients with Burkitt's lymphoma. The great majority of the carcinomas were from the face and neck regions. Lymphocytes from the blood of the majority of patients with Burkitt's lymphoma, as well as those from tumor tissues, exhibited a reduced cap-forming ability (2-6%) and increased Con-A-induced agglutinability compared to lymphocytes from healthy normal donors and from patients with carcinoma, although some of the lymphocytes from patients with carcinoma had a somewhat lower range of cap formation than the lymphocytes from healthy donors. No difference was observed in the interaction with Con A of lymphocytes from the different types of carcinoma studied. Eight lymphoid cell lines were established in our laboratory from the tumor tissues of patients with Burkitt's lymphoma. The cap-forming ability and agglutinability by Con A of these lines was examined and compared to those of the "classical" lymphoma lines: Raji, Daudi and P3HR1. All cell lines exhibited an increased Con-A-induced agglutinability and a reduced cap-forming ability compared to normal lymphocytes, except for P3HR1 cells which exhibited a cap-forming ability of 15-20%. These findings are discussed in relation to the association of the lymphocytes with malignancy and as a possible aid in the differential diagnosis between malignant lymphomas and other diseases.

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Section: Discussionmentioning

confidence: 86%

Section: Lymphomamentioning

confidence: 92%

Concanavalin a receptors on the surface membrane of lymphocytes from patients with african Burkitt's lymphoma and lymphoma cell lines

Ben‐Bassat

Goldblum

Mitrani

et al. 1976

Intl Journal of Cancer

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“…Since institutionalization by itself, and the factor of old age are known to accelerate the decline of immune functions in DS subjects [Franceschi et al, 1978;Whittingham et al, 1977] it is reasonable to expect that the older institutionalized DS subjects studied by Naeim and Walford [ 1980] should have more severe lymphocyte defects than the younger DS subjects studied by us. Finally, Naeim and Walford [1980] recorded the percentage of capped cells after 90 min of incubation at 37 °C with FITC-Con A, while it is usual to study Con A capping in human lymphocytes at earlier times [Ben Bassat and Goldblum, 1975;Ben Bassat et al, 1977;Pickard et al, 1978;Bhalla et al, 1979;Noronha et al, 1980], A longer incubation time may lead to an underestimation of the capped cells since capping is followed by endocytosis or shed ding of the fluorescent cap.…”

Section: Discussionmentioning

confidence: 99%

Alterations of the Capping Phenomenon on Lymphocytes from Aged and Down’s Syndrome Subjects

Chiricolo¹,

Minelli²,

Licastro³

et al. 1984

Gerontology

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The redistribution with the time of concanavalin A (Con A) receptors at one pole of the cell after addition of FITC-Con A – so-called capping – in the peripheral blood lymphocytes from 13 aged subjects (mean: 84 ± 1 years old), and of 16 noninstitutionalized patients affected by a syndrome of precocious aging, such as Down’s syndrome (mean: 17 ± 2 years old), was studied and compared with a group of 15 normal young people (mean: 23 ± 2 years old). An opposite alteration in the percentage of capped cells, i.e. a decrease in aged subjects and an increase in Down’s syndrome patients, was observed. A derangement of lymphocyte membrane fluidity appears to be present in both groups even if the underlying biochemical defect may be different. However, a similar alteration of the kinetics of the phenomenon was present either in aged or in Down’s syndrome subjects. Both groups did not show any significant increase with time of the percentage of capped cells, suggesting that they were lacking a lymphocyte subpopulation(s) which start capping later.

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“…The cell concentrations were lo6, 2 x lo6 and 5 x lo6 cells per ml in a 35-mm Petri dish. The density and size of aggregates were scored visually on a scale from -to +++ after a 30-min incubation at 24" C with occasional shaking (Ben-Bassat and Goldblum, 1975).…”

Section: Agglutination By Concanavalin Amentioning

confidence: 99%

Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia

Klein¹,

Vánky²,

Ben‐Bassat³

et al. 1976

Intl Journal of Cancer

355

179

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The K562 cell line derived from a CML patient in blast crisis was examined for properties of B and T lymphocytes and cell lines. K562 lacks the B markers of immunoglobulins, Epstein-Barr virus (EBV) genome and associated nuclear antigen, and receptors for EBV. A low proportion of cells from rosettes with sheep erythrocytes, the frequency of which is considerably increased after neuraminidase treatment. Unlike B lines but like T lines, K562 cells are lysed rapidly by C'/Fc receptor-positive human blood leukocytes and do not stimulate MLC reactions. On the other hand, K562 lacks T antigen, high radiosensitivity and sensitivity to growth inhibition by thymidine. The cells do not contain N-APase, an enzyme found in all lines derived from lymphoid cells and in lymphoproliferative diseases. By scanning electron microscopy, K562 cells were seen to be rounded and relatively smooth, with small numbers of short microvilli resembling undifferentiated leukemic cells. A few cells had narrow ridge-like profiles and small ruffles similar to granulocytic leukemic cells. K562 is strongly positive for immunoglobuln Fc receptors and pinocytosis, but does not phagocytose or mediate antibody-dependent phagocytosis or cytolysis. Among histochemical stains, K562 is positive for esterase, lipid, and acid phosphatase. There seems to be no doubt that K562 is not a B cell line. While it has some T cell properties, these are not exclusive. Some of its characteristics indicate that it is probably not lymphoid. Due to its low level of differentiation, its nature cannot be stated with certainty. On the basis of the possible presence of the cellular marker of chronic myeloid leukemia, the Ph chromosome, it may be regarded as belonging to the granulocytic series of cells.

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Concanavalin A receptors on the surface membrane of lymphocytes from patient's with Hodgkin's disease and other malignant lymphomas.

Cited by 36 publications

References 17 publications

Concanavalin a receptors on the surface membrane of lymphocytes from patients with african Burkitt's lymphoma and lymphoma cell lines

Concanavalin a receptors on the surface membrane of lymphocytes from patients with african Burkitt's lymphoma and lymphoma cell lines

Alterations of the Capping Phenomenon on Lymphocytes from Aged and Down’s Syndrome Subjects

Properties of the K562 cell line, derived from a patient with chronic myeloid leukemia

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