Concanavalin A Derivatives with Altered Biological Activities

Gunther, Gary R.; Wang, John L.; Yahara, Ichiro; Cunningham, Bruce A.; Edelman, Gerald M.

doi:10.1073/pnas.70.4.1012

Cited by 377 publications

(218 citation statements)

References 23 publications

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“…Since the identical dose-response curves were unexpected , we carried out hemagglutination and tnitogenicity tests to assure the authenticity of our succiny l Con A prepar ation ; in both assays the results agreed well with literature values [6].…”

Section: T Ox I N -R E C E P T O R B I N D I N G Assaysupporting

confidence: 70%

“…Con A has been shown to inhibit patching and capp ing of various surface receptors on lymphocytes and numerous established cell lines [4, 141 . This inhibition is reversed by microtubule depolymerizing agents such as coichicine [7] and canno t be elicited b y succiny l. Con A [4,6], a monovalent derivative of Con A . As assayed in our system , Con A and succi nyl Con A are eq ua ll y effective at altering the 37°C binding pat tern.…”

Section: In This Work We Have S Tu D Ie D T H E E F F E C T S Of Lectmentioning

confidence: 99%

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Effects of lectins on the interaction of diphtheria toxin with mammalian cells

Middlebrook¹,

Dorland²,

Leppla³

1979

Experimental Cell Research

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Section: T Ox I N -R E C E P T O R B I N D I N G Assaysupporting

confidence: 70%

Section: In This Work We Have S Tu D Ie D T H E E F F E C T S Of Lectmentioning

confidence: 99%

Effects of lectins on the interaction of diphtheria toxin with mammalian cells

Middlebrook¹,

Dorland²,

Leppla³

1979

Experimental Cell Research

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“…The decreased thymidine uptake observed at high lectin concentrations appears to correlate with impaired lymphocyte survival (Fig. 7 B), perhaps a result of the inactivation of surface receptors necessary for membrane function (23). Little correlation was observed in Con A-stimulated lymphocyte cultures between DNA synthesis and MAF production; these may constitute two independent phenomena triggered simultaneously by lectins.…”

Section: Discussionmentioning

confidence: 81%

Studies on the mechanisms of macrophage activation. II. Parasite destruction in macrophages activated by supernates from concanavalin A-stimulated lymphocytes.

Büchmuller¹,

Mauël²

1979

The Journal of Experimental Medicine

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Activation of mouse peritoneal exudate macrophages, as evidenced by destruction of the intracellular protozoan parasite Leishmania enriettii, was obtained by incubation with supernates from concanavalin A (Con A)-stimulated syngeneic spleen cells. Parasites were not destroyed in macrophages exposed to control media. Supernate-induced activation was independent of the presence of Con A. The activating principle (macrophage activating factor, or MAF) was produced by Con A-stimulated lymphocytes in presence or absence of serum. In absence of serum, MAF synthesis was highest at Con A concentrations far below those required in serum-containing media. MAF production was reduced at Con A concentrations of 10 microgram/ml or above, probably a result of toxicity of the lectin for lymphocytes. MAF was detectable after 24 h of lymphocyte stimulation and increased up to 72 h; production appeared to be independent of DNA synthesis. Serum-free MAF was inactive when tested as such on macrophages. Full activity could be restored by addition of nanogram amounts of endotoxin or of FCS before assay. Endotoxin also considerably potentiated MAF activity in serum-containing supernates. Full intracellular parasite destruction was observed after contact of macrophages with MAF for 20 h. The continuous presence of MAF was not necessary for activation; a 10-h pulse was sufficient to induce macrophages to destroy all intracellular microorganisms within the next 38 h.

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“…Effect of Chemical modification on haemagglutinating activity of the field bean or lablab bean lectin. On the other hand acetylation, and succinylation of Con A did not alter its lectin activity as well as its antigenicity (17,18). Citraconylation of potato lectin also did not alter its biological activity (19).…”

Section: Modification Of Histidinementioning

confidence: 93%

Chemical modification studies on the glucose/mannose specific lectins from field and lablab beans

Nadimpalli

1999

IUBMB Life

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Summary : Seeds of Dolichos lablab var. lignosus (field beans) and variety typicus(lablab beans) contain glucose/mannose specific leetins that have been affinity purified and well eharactefised (Siva Kumar N., and Rajagopal Rao, D., J. Biosei., 1986, 10, 95-109, (1) Rajasekhar etaL, (Biochem.Archives. 1997 (2). Purified lectins are glycoproteins with a native molecular mass of 60 kDa and are made of two types of subunits (Gowda etal., 1994, J.Biol.Chem. 269, 18789-18793) (3). Chemical modifications of various groups in purified lectins (using group specific reagents) such as lysine (eitraeonie anhydride), carboxyl groups (water soluble earbodi-imide) tyrosine (N-aeetyl imidazole) and tryptophan (2-hydroxy 5-nitro benzylbromide) revealed that 14 out of 21 residues of lysines 7 out of 92 residues of earboxyl groups, 16 out of 24 tyrosine residues and 2 out of 10 tryptophan residues were modified. Lysine and earboxyl group modification led to 95% loss in haemaglutinafing activity compared to control while tyrosine and tryptophan modifications led to complete loss of lectin activity. Arginine and histidine modifications led to only 50% loss in activity. The extent of modification and loss in activity was same when the lysine and carboxyl groups were modified in the presence and absence of the inhibitory sugar methyl ct-D-glucopyranoside at 0.1M Concentration. However protection of modification and lectin activity was observed when the tyrosine and tryptophan residues were modified in the presence of the inhibitory sugar. Earlier CD studies carried out (1) and extensive chemical modification studies reported here substantiate the involvement of tyrosine and tryptophan residues in the sugar binding site of these lectins.

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Concanavalin A Derivatives with Altered Biological Activities

Cited by 377 publications

References 23 publications

Effects of lectins on the interaction of diphtheria toxin with mammalian cells

Effects of lectins on the interaction of diphtheria toxin with mammalian cells

Studies on the mechanisms of macrophage activation. II. Parasite destruction in macrophages activated by supernates from concanavalin A-stimulated lymphocytes.

Chemical modification studies on the glucose/mannose specific lectins from field and lablab beans

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