COMSOL-Based Design and Validation of Dilution Algorithm with Continuous-Flow Lab-on-Chip

Banerjee, Tapalina; Ghoshal, S. K.; Bhattacharya, Bhargab B.

doi:10.1007/s41403-017-0022-z

Cited by 7 publications

(11 citation statements)

References 15 publications

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“…Any defect in elastic micro-valves or micro-pumps may also introduce various microfluidic errors in valvebased CFMBs [2], [3]. Free-flowing programmable architectures that obviate the need for valves as well as split operations were proposed in [4], [20], [21] to overcome these limitations. The work in [4] studies a 2D microfluidic network for implementing dilution on a free-flowing biochip.…”

Section: Preliminaries Of Sample Preparationmentioning

confidence: 99%

“…Free-flowing programmable architectures that obviate the need for valves as well as split operations were proposed in [4], [20], [21] to overcome these limitations. The work in [4] studies a 2D microfluidic network for implementing dilution on a free-flowing biochip. In order to capture subtleties of mixing and diffusion phenomena in a more realistic fashion [31], a 3D-model for the fluidic network was presented later [20].…”

Section: Preliminaries Of Sample Preparationmentioning

confidence: 99%

“…Note that the volume and injection velocity of fluids in a channel network can be controlled by choosing appropriate physical dimensions of input ports and input pressure. In previous approaches to preparing dilution with free-flowing biochips [4], [20], a large volume of fluids is wasted initially before the stablity of flow is reached at the sink-outlet(s). Also, fluid-injection velocities at different input ports along with their corresponding injection times, play a vital role in achieving the stability and accuracy of target-CF.…”

Section: Motivationmentioning

confidence: 99%

“…Also, fluid-injection velocities at different input ports along with their corresponding injection times, play a vital role in achieving the stability and accuracy of target-CF. Adjustment of such velocities and timing is a challenging task [4], [20].…”

Section: Motivationmentioning

confidence: 99%

“…A simpler technology for designing CFMBs is to use free-flowing fluid through rigid channels, where no control layer or valves are needed [4]- [7]. Free-flowing biochips only require a switching mechanism to inject or stop fluids at the inlets (or input ports).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sample Preparation Meets Farey Sequence: A New Design Technique for Free-Flowing Microfluidic Networks

Banerjee¹,

Poddar²,

Ho³

et al. 2021

Preprint

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Design of microfluidic biochips has led to newer challenges to the EDA community due to the availability of various flow-based architectures and the need for catering to diverse applications such as sample preparation, personalized medicine, point-of-care diagnostics, and drug design. The ongoing Covid-19 pandemic has increased the demand for low-cost diagnostic lab-on-chips manifold. Sample preparation (dilution or mixing of biochemical fluids) is an indispensable step of any biochemical experiment including sensitive detection and successful assay execution downstream. Although for valve-based microfluidic biochips various design automation tools are currently available, they are expensive, and prone to various manufacturing and operational defects. Additionally, many problems are left open in the domain of free-flowing biochips, where only a single layer of flow-channels is used for fluid-flow devoid of any kind of control layer/valves. In this work, we present a methodology for designing a free-flowing biochip that is capable of performing fluid dilution according to user's requirement. The proposed algorithm for sample preparation utilizes the Farey-sequence arithmetic of fractions that are used to represent the concentration factor of the target fluid. We also present the detailed layout design of a free-flowing microfluidic architecture that emulates the dilution algorithm. The network is simulated using COMSOL multi-physics software accounting for relevant hydrodynamic parameters. Experiments on various test-cases support the efficacy of the proposed design in terms of accuracy, convergence time, reactant cost, and simplicity of the fluidic network compared to prior art.

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Section: Preliminaries Of Sample Preparationmentioning

confidence: 99%

Section: Preliminaries Of Sample Preparationmentioning

confidence: 99%

Section: Motivationmentioning

confidence: 99%

Section: Motivationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sample Preparation Meets Farey Sequence: A New Design Technique for Free-Flowing Microfluidic Networks

Banerjee¹,

Poddar²,

Ho³

et al. 2021

Preprint

Self Cite

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Separation of particles in spiral micro-channel using Dean’s flow fractionation

Mitra

Dutta

Nagahanumaiah

et al. 2020

J Braz. Soc. Mech. Sci. Eng.

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Separation of large-sized micro-particles or biological cells inside micro-fluidic environment has significant biomedical applications. In the present work, a spiral micro-channel of comparatively larger dimension has been used for separation of polymeric micro-particles based on their dimensions. A spiral micro-channel can also be effectively used for large-sized biological cell or circulating tumour cell (CTC) isolation. Conventional methods are generally used to fabricate small and precise micro-channels to achieve such separation. This requires sophisticated (mostly lithographic) fabrication facility which is time-consuming and costly. In the present study, a novel approach has been introduced to fabricate spiral microchannel. A low-cost laser machining method has been used for fabricating the micro-channel that works on the concept of Dean's flow fractionation for particle separation. In order to investigate the Dean's flow-based separation process, we have modelled the overall process on a computational fluid dynamics (CFD) platform and have done the necessary simulations. For understanding the trajectories of particle flow inside the channel, we have done particle tracing study to reveal the physics of separation process based on Dean's flow.

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Water and hydrogen transport modelling through the membrane-electrode assembly of a PEM fuel cell

Ionescu

2020

Phys. Scr.

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Membrane electrode assembly (MEA) formed by a proton exchange membrane (PEM), two catalyst layers and two gas diffusion layers represent the heart of the PEM fuel cell system, being the place where the electrochemical reactions are developed in order to generate electrical power. Due to a complex water production and transportation process through the porous media of MEA, the water management represents one of the most critical issues for the low temperature PEM fuel cell systems, working at 30 °C-80 °C. In the present paper, a one-dimensional mass transport PEMFC model for evaluation of net water flux across the Nafion type membrane was implemented with the Comsol Multiphysis software, based on the finite element method. Hydrogen crossover phenomenon, which may cause a degradation of the reaction sites inside MEA, was also included in the model. The numerical model offered a reasonable prediction of the fuel cell electrical performance in the form of current-voltage characteristic after validation with experimental data from the literature. Fuel cell performance was investigated by modifying the temperature and pressure inside the cell, along with the relative humidity for reactant gases.

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COMSOL-Based Design and Validation of Dilution Algorithm with Continuous-Flow Lab-on-Chip

Cited by 7 publications

References 15 publications

Sample Preparation Meets Farey Sequence: A New Design Technique for Free-Flowing Microfluidic Networks

Sample Preparation Meets Farey Sequence: A New Design Technique for Free-Flowing Microfluidic Networks

Separation of particles in spiral micro-channel using Dean’s flow fractionation

Water and hydrogen transport modelling through the membrane-electrode assembly of a PEM fuel cell

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