Computerized Morphometric Assessment of the Effect of Low-Level Laser Therapy on Bone Repair: An Experimental Animal Study

Júnior, Aurelício Novaes Silva; Pinheiro, António Luiz Barbosa; Oliveira, Marília Gerhardt de; Weismann, Rubén; Ramalho, Luciana Maria Pedreira; Nicolau, Renata Amadei

doi:10.1089/104454702753768061

Cited by 129 publications

(85 citation statements)

References 23 publications

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“…This laser stimulation vitalizes the cell by increasing mitochondrial ATP production. 22 The present results agree with those reported by Silva Junior et al 18 and Lopes et al 20 in terms of the wavelength used but differ from the findings of Dortbudak et al, 17 who used a longer wavelength.…”

Section: Low-level Laser Therapy and Calcitonin In Bone Repairsupporting

confidence: 75%

“…In vivo and in vitro studies have shown the biological effects of laser therapy such as an increase in local microcirculation, activation of the lymphatic system, and proliferation of epithelial cells and fibroblasts. [17][18][19][20] Pinheiro and Gerbi 12 discussed the positive effects of laser irradiation on the cell membrane and mitochondria and its influence on adenosine triphosphate (ATP) production. Bone tissue is a major source of angiogenic and endothelial factors, as well as bone morphogenetic proteins, which are essential for osteogenesis and angiogenesis.…”

Section: Low-level Laser Therapy and Calcitonin In Bone Repairmentioning

confidence: 99%

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Effect of Low-Level Laser Therapy and Calcitonin on Bone Repair in Castrated Rats: A Densitometric Study

Nascimento

Cardoso

Ribeiro

et al. 2010

Photomedicine and Laser Surgery

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Objective: To investigate the healing of bone defects in male rats treated with salmon calcitonin, low-level laser therapy (LLLT), or both. Background: Healing of bone defects still represents a challenge to health professionals in several areas. In this article, the effect of calcitonin in combination with LLLT on bone repair was studied. Densitometry was used as a valuable tool for the measurement of bone regeneration. Methods: Sixty male Wistar rats underwent bilateral castration surgery before the creation of a surgical bone defect. The animals were randomly divided into four groups: control, treated with calcitonin (Ca), treated with LLLT (La), and treated with calcitonin and LLLT (CaLa). Groups Ca and CaLa received 2 IU=kg of synthetic salmon calcitonin intramuscularly three times a week. Groups La and CaLa received laser therapy using a gallium-aluminum-arsenide laser (10 mW, 20 J=cm 2 , wavelength 830 nm). Control animals were submitted to sham irradiation. The animals were sacrificed 7, 14, and 21 days after surgery, and bone defects were analyzed using densitometry. Results: The CaLa group had a higher degree of bone regeneration 14 and 21 days after surgery. Conclusions: The La and CaLa had significantly higher bone mineral density than the control and Ca groups.

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Section: Low-level Laser Therapy and Calcitonin In Bone Repairsupporting

confidence: 75%

Section: Low-level Laser Therapy and Calcitonin In Bone Repairmentioning

confidence: 99%

Effect of Low-Level Laser Therapy and Calcitonin on Bone Repair in Castrated Rats: A Densitometric Study

Nascimento

Cardoso

Ribeiro

et al. 2010

Photomedicine and Laser Surgery

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“…Session and treatment doses were also effective as previously described by our team 1,3,5 and by other groups using IR laser radiation on bone healing. 11,[27][28][29] In conclusion, the use of laser photobiomodulation was effective in improving bone healing as a result of increased deposition of CHA as measured by Raman spectroscopy and SEM analysis.…”

Section: Discussionmentioning

confidence: 83%

Infrared Laser Photobiomodulation (λ 830 nm) on Bone Tissue Around Dental Implants: A Raman Spectroscopy and Scanning Electronic Microscopy Study in Rabbits

Lopes

Pinheiro

Sathaiah

et al. 2007

Photomedicine and Laser Surgery

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Objective: The aim of this study was to assess, through Raman spectroscopy, the incorporation of calcium hydroxyapatite (CHA; ~960 cm -1 ), and scanning electron microscopy (SEM), the bone quality on the healing bone around dental implants after laser photobiomodulation (830 nm). Background Data: Laser photobiomodulation has been successfully used to improve bone quality around dental implants, allowing early wearing of prostheses. Methods: Fourteen rabbits received a titanium implant on the tibia; eight of them were irradiated with 830 nm laser (seven sessions at 48-h intervals, 21.5 J/cm 2 per point, 10 mW, ~0.0028 cm 2 , 86 J per session), and six acted as control. The animals were sacrificed 15, 30, and 45 days after surgery. Specimens were routinely prepared for Raman spectroscopy and SEM. Eight readings were taken on the bone around the implant. Results: The results showed significant differences on the concentration of CHA on irradiated and control specimens at both 30 and 45 days after surgery ( p < 0.001). Conclusion: It is concluded that infrared laser photobiomodulation does improve bone healing, and this may be safely assessed by Raman spectroscopy or SEM.

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“…LLLT accelerates osteoblast proliferation, bone formation [31], and bone repair [32]. Various groups have suggested the involvement of insulin-like growth factor 1 (IGF-1) [33], mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) [34], and bone morphogenetic protein (BMP)/Smad signaling cascades [35].…”

Section: Bone Growth and Repairmentioning

confidence: 99%

Biological Function of Low Reactive Level Laser Therapy (LLLT)

Kubota¹,

Ishihara²

2017

Photomedicine - Advances in Clinical Practice

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Low reactive level laser therapy (LLLT) and photobiomodulation are mainly focused on the activation of intracellular or extracellular photoabsorbable molecule (chromophore) and the initiation of cellular signaling using low power lasers and lights. Over the past 40 decades, a number of basic and clinical researches were reported that the laser therapy had the potential to improve wound healing and reduce pain and inflammation. In recent years, the term "LLLT" has become widely recognized. In this review, the mechanisms of action of LLLT at a cellular level are described. Finally, our recent research results that LLLT enhanced the cells differentiation are also described.

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Computerized Morphometric Assessment of the Effect of Low-Level Laser Therapy on Bone Repair: An Experimental Animal Study

Abstract: It is concluded that, under this experimental condition, LLLT increased bone repair at early bone healing.

Cited by 129 publications

References 23 publications

Effect of Low-Level Laser Therapy and Calcitonin on Bone Repair in Castrated Rats: A Densitometric Study

Effect of Low-Level Laser Therapy and Calcitonin on Bone Repair in Castrated Rats: A Densitometric Study

Infrared Laser Photobiomodulation (λ 830 nm) on Bone Tissue Around Dental Implants: A Raman Spectroscopy and Scanning Electronic Microscopy Study in Rabbits

Biological Function of Low Reactive Level Laser Therapy (LLLT)

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