Computerized analysis of erythrocyte aggregation from sequential video-microscopic images under gravitational sedimentation

Jayavanth, Sanjay; Singh, Megha

doi:10.1016/j.rbmret.2003.12.002

Cited by 12 publications

(6 citation statements)

References 19 publications

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“…The possible shapes into which an erythrocyte can deform in various blood vessels were proposed in Schmid‐Schönbein and Grunau 9 . Several of the deformed RBC shapes were visualized by in vitro techniques such as rheoscopy, 20,23 capillary flow and fixation, 21 microchannel flow, 11–19 and also through in vivo techniques 9,22 . Most of these studies reported either the axially deformed elliptical shapes 14,20,23 or the asymmetrically deformed parachute shape 19,22 using sophisticated methods.…”

Section: Resultsmentioning

confidence: 99%

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A Microchannel for in Vitro Imaging of Erythrocyte Shape Transformations by Video Microscopic Technique

Jayavanth

Lee

Pak

2008

Experimental Techniques

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Section: Resultsmentioning

confidence: 99%

“…This ensured restoration of continuous edges without increasing the size of the object. Details of this method are similar to those discussed else where 16 …”

Section: Methodsmentioning

confidence: 99%

A Microchannel for in Vitro Imaging of Erythrocyte Shape Transformations by Video Microscopic Technique

Jayavanth

Lee

Pak

2008

Experimental Techniques

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“…Projected aggregate area (PPA), perimeter (PAP) and form factor (FF), H = 5% [17]. MAI = Units in sample/Units in Ringer solution, H = 1% [7] EAI = Total RBC-aggregate area/Area of aggregates, H = 0.5% [12].…”

Section: Hematocritmentioning

confidence: 99%

Mechanics of the red blood cell network

Kaliviotis

2015

JCB

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Changes in the microstruture of blood caused by the red blood cell aggregation phenomenon affect the mechanical properties of the fluid. This fact has been extensively investigated in the past and it has been verified that the formation of rouleaux (the characteristic coin-pile like structures) and aggregates at low shear rates affect the flow and as a consequence the viscosity of the fluid is elevated. At physiological red blood cell concentrations and at low shear rates, however, in addition to rouleaux and aggregate formation an extended network is observed. The effects of network characteristics on the mechanical properties of blood have received relatively little attention in the literature and only a few studies provide quantitative data on this aspect of the blood flow. Quantitative data on network characteristics in view of the aggregation phenomenon illustrate that indeed different network configurations have different effects on the mechanical properties of the fluid. In this work, it is shown that changes in the viscosity of blood at specific flow conditions correlate with the changes in the mechanical characteristics of the red blood cell network.

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“…In fact, RBCs that do not meet the deformability requirements are retained in the spleen. Several studies have reported correlation of reduced erythrocyte deformability to a number of cardiovascular diseases and disorders, as well as according to their severity [1][2][3][4][5][6][7][8][9][10]. Reduced deformability is an important contributing factor in cardiovascular complications.…”

Section: Introductionmentioning

confidence: 99%

“…Blood rheology, both in in vivo and in vitro models, has been studied by several authors [5][6][7][8][9][10]. Hitherto, methods to measure erythrocyte deformability include viscosimetric, filtration technique, and micropipette aspiration.…”

Section: Introductionmentioning

confidence: 99%

Multi-shape erythrocyte deformability analysis by imaging technique

2010

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In their extensive circulation through the cardiovascular circuit, erythrocytes are forced to transform and assume different shapes depending on the varying flow conditions in different blood vessels. The present work aims to visualize various erythrocyte shape transformations by an in vitro microcirculatory model, and to visualize multi-shape erythrocyte deformability. The model used an in-house fabricated and inexpensive disposable micro flow channel to mimic certain in vivo conditions, and a fast frame rate video microscopic system to visualize the shape changes in erythrocytes. Results have shown the multi-shape transformation of erythrocytes as resting discoidal shape, asymmetrically deformed 'hat' and 'bullet-like' shapes, and axially deformed 'slipper-and spindle-like' shapes. Specific erythrocytes demonstrated shape transition and transformation while passing through the observed window. The obtained erythrocyte shaper were thoroughly analyzed based on the deformability index. Findings from the image processing techniques were significant (P<0.001) for the different shapes compared with the resting shape. The simple glass in-house fabricated microfludic channel allowed for multi-shape erythrocyte deformability characterization, many of which manifest in human circulation.

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Computerized analysis of erythrocyte aggregation from sequential video-microscopic images under gravitational sedimentation

Cited by 12 publications

References 19 publications

A Microchannel for in Vitro Imaging of Erythrocyte Shape Transformations by Video Microscopic Technique

A Microchannel for in Vitro Imaging of Erythrocyte Shape Transformations by Video Microscopic Technique

Mechanics of the red blood cell network

Multi-shape erythrocyte deformability analysis by imaging technique

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