Computerised multiparametric analysis from images of blood flow through frog mesenteric arterial bifurcation

Abstract

A computerised multiparametric procedure is developed to analyse the images of blood flow through various locations of the mesenteric arterial bifurcation of frog. The data are recorded by a video microscopic system and, after digitisation and pre-processing, are analysed by an IBM PC/AT based image processing system to obtain erythrocyte and velocity distribution profiles by axial tomographic and image velocimetry techniques, respectively. The vessel radius, haematocrit, blood flow through main and branch arteries and flow separation zones are determined from the data by various analytical procedures. In contrast to the earlier techniques the data are obtained from the same location of the vessel and thus the variability in flow parameters is minimised.

Computerised visualisation from images of blood flow through frog mesenteric microvessels with multiple complexities

“…motion segmentation and matching region decision (CORNELIUS and KANADE, 1981;SCHUNCK, 1984). Further details of the modification procedure are given elsewhere (UMRANI et al, 1997). where Ex, Ey and Et denote the partial derivatives of the image brightness with reference to the x-and y-directions and time t, respectively.…”

“…The computed displacements were in good agreement with the given displacement of the test image. Further details of this procedure and a flow chart are given elsewhere (PRAKASH and SINGH, 1996;UMRANI et al, 1997). For the determination of velocity profiles, the digitised serial images of 128 x 128-pixel resolution, with 256 grey levels, were used.…”

“…Generally, the blood flow-related parameters are obtained from their microscopic images. The measured parameters include red cell velocity (BAKER and WAYLAND, 1974;SEKI and LIPOWSKI, 1989;LOMINADZE and MCHEDLISHVILI, 1999), vessel diameter (PRIES and GAEHTGENS, 1987;UMRANI et al, 1997) and vessel haematocrit (LIPOWSKI et al, 1980). Blood flow through a microvessel is influenced by the presence of various complexities.…”

Detection of pulsatile blood flow cycle in frog microvessels by image velocimetry

“…The computed displacements were in good agreement with the given displacement of the test image. Further details of this procedure and a flow chart are given elsewhere (UMRANI et al, 1997).…”

“…The image velocimetry procedure, which is based on the distribution of the apparent velocities of movement of a brightness pattern in an image, is applied to analyse the distribution of velocities in an arterial bifurcation under steady blood flow conditions (UMRANI et al, 1997). This is a non-contact procedure that could further be applied to analyse the cyclic variation of the pulsatile blood flow through a microvessel in the absence of the EGG, from a sequence of recorded images covering more than one flow cycle.…”