Computer software for identification of honey bee subspecies and evolutionary lineages

Nawrocka, Anna; Kandemi̇r, İrfan; Fuchs, Stefan; Tofilski, Adam

doi:10.1007/s13592-017-0538-y

Cited by 35 publications

(80 citation statements)

References 35 publications

(62 reference statements)

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“…Moreover, the identification of the subspecies is important for their conservation (De la Rúa et al 2009). Such identification is often based on measurements of various body parts including legs and sternite, but wing measurements only are usually sufficiently precise (Kandemir, Özkan, & Fuchs, 2011;Francoy et al, 2008;Tofilski, 2008;Gerula et al, 2009;Nawrocka et al, 2018). There is a significant correlation between morphometric and molecular methods (Miguel et al, 2011;Oleksa & Tofilski, 2015).…”

Section: Introductionmentioning

confidence: 99%

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies

Węgrzynowicz

Gerula

Tofilski

et al. 2019

Journal of Apicultural Science

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The identification of honey bee (Apis mellifera) subspecies is often based on the measurements of workers’ fore-wings. The interpretation of the measurements can be difficult because the phenotype of workers is affected by both genetic and environmental factors. Moreover, it is not clear how the phenotype is affected by maternal inheritance. We have used the methodology of geometric morphometrics to verify if hybrids of honey bee subspecies and their backcrosses are more similar to either the father or mother colony. The comparison was based on fore-wing venation of three honey bee subspecies: A. m. carnica, A. m. caucasica, A. m. mellifera. First generation hybrids and backcrosses of those subspecies were obtained through instrumental insemination. Workers of the hybrids were compared with their parental colonies. The shape of wing venation was more similar to the maternal than to parental colony. This phenomenon was particularly visible in first generation of hybrids but it was also present in backcrosses. There were also symptoms of genetic dominance of some subspecies but this effect interacted with maternal inheritance and was difficult to interpret.

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Section: Introductionmentioning

confidence: 99%

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies

Węgrzynowicz

Gerula

Tofilski

et al. 2019

Journal of Apicultural Science

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“…Discrimination between the honeybee subspecies is useful for the conservation of their biodiversity, for monitoring of local honeybees and, also, for limiting the introduction of non-native subspecies. (Nawrocka et al, 2017). The methods designed to identify different honeybee subspecies rely on morphometric measurements of the entire honeybee body, including measures of some anatomic structures, the venation angles of the wing and the pigmentation.…”

Section: Introductionmentioning

confidence: 99%

“…Computerized methods using wing image are the most promising, reducing the number of dissected body parts and also avoiding the laborious preparation of sternites, mouthparts and legs (Nawrocka et al, 2017). The automated identification of bee through wing images involves several steps such as: obtaining the image of the honeybee wing -high-quality wing images can be obtained using relatively inexpensive scanners (MacLeod et al, 2007), processing the wings image (settlement of the 19 points), and classification and validation, (Santana et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The automated identification of bee through wing images involves several steps such as: obtaining the image of the honeybee wing -high-quality wing images can be obtained using relatively inexpensive scanners (MacLeod et al, 2007), processing the wings image (settlement of the 19 points), and classification and validation, (Santana et al, 2014). Software packages were developed for the automated identification of the honeybee subspecies like: ABIS (Automated Bee Identification System) ((Leno da Silva, et al, 2015); ID-BEES; FABIS (Nawrocka, et al, 2017); DrawWing system (Tofilski, 2008); and the ApiClass system (Nawrocka et al, 2017;Baylac et al, 2008). The first three software are not usually available to the general public, but the last two software are (Nawrocka et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Software packages were developed for the automated identification of the honeybee subspecies like: ABIS (Automated Bee Identification System) ((Leno da Silva, et al, 2015); ID-BEES; FABIS (Nawrocka, et al, 2017); DrawWing system (Tofilski, 2008); and the ApiClass system (Nawrocka et al, 2017;Baylac et al, 2008). The first three software are not usually available to the general public, but the last two software are (Nawrocka et al, 2017). The DrawWing system uses (Tofilski, 2008) the junctions of veins as landmarks for the geometrical morphometry (Oleksa et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of the Honeybee Subspecies From Some Romanian Counties Using a Semiautomatic System for Analyzing Wings

Buescu

Gurău

Daneş

2018

CBUP

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Morphometric analysis of honeybee wings can be used to discriminate between honeybee subspecies. The classic morphometric methods used to identify honeybee subspecies are time consuming while geometric morphometric analysis are proven to be more efficient and rapid for the identification of honeybee subspecies. Even if the geometric morphometric analysis involves several steps such as: obtaining the image of the honeybee wing, processing the wings image (settlement of the 19 points), classification and validation, in terms of consumed time,it takes less than classic morphometric methodes and it is easier to apply. The aim of this study was to identify honeybee subspecies from the Romanian South-Eastern area, using the geometric morphometric method. To accomplish this, the semiautomated French system ApiClass was used. This system allows us to identify the honeybee subspecies based on the wing's image of the Apis mellifera honeybee worker. The program is using the recent approaches of geometric morphometry and is analyzing the wing considering 19 points corresponding to the intersections of the main veins of the bee forewings. These coordinates are processed by the system before being analyzed after which the system returns the probability of the analyzed honeybee belonging to one of the honeybee subspecies from its reference system. The system has more than 5000 honeybee wing references off the main lines and honeybee subspecies. Using this system we analyzed samples from the counties of Braila, Buzau, Galati and Ilfov. Each sample was composed from wings of 20 honeybees. Most of the analyzed samples were identified as hybrids without being possible to specify the type or the level of the hybridization: only three samples were identified as being Apis mellifera carnica presenting a homology degree over 90%.

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Insights into Ethiopian honey bee diversity based on wing geomorphometric and mitochondrial DNA analyses

et al. 2020

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Traditional beekeeping has been playing important socio-economic roles in Ethiopia for millennia. The country is situated in northeast Africa, where ranges of major evolutionary lineages of Apis mellifera adjoin. However, studies on the classification and distribution of subspecies and lineages of honey bees in the country are partly inconsistent, either proposing multiple subspecies and lineages or a unique A. m. simensis. This study was conducted with the aim of elucidating Ethiopian honey bees in reference to African subspecies and major global lineages using wing geometric morphometrics and COI-COII mitochondrial DNA analyses. For this purpose, 660 worker bees were collected from 66 colonies representing highland, midland, and lowland zones in different locations. Both methods indicated that the samples from this study form a distinct cluster together with A. m. simensis reference. In addition, forewing venation patterns showed that most of the Ethiopian samples are separate from all reference subspecies, except A. m. simensis. Analysis of COI-COII sequences revealed five DraI haplotypes (Y2, Y1, A1, and O5’), of which one was new denoted as Y3. Moreover, centroid size strongly associated with elevation. In conclusion, the results supported that Ethiopian honey bees are distinct both at lineage and subspecies levels; however, there is an indication of lineage O in the north.

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Computer software for identification of honey bee subspecies and evolutionary lineages

Cited by 35 publications

References 35 publications

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies

Maternal Inheritance in Hybrids of Three Honey Bee Subspecies

Identification of the Honeybee Subspecies From Some Romanian Counties Using a Semiautomatic System for Analyzing Wings

Insights into Ethiopian honey bee diversity based on wing geomorphometric and mitochondrial DNA analyses

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