Computer Simulation of Isotachophoresis

Abstract

A computer simulation of isotachophoresis is described for the purpose of determining physico-chemical constants and separation optimization. On the basis of the mobilities, dissociation constants and stability constants of the separands, the effective mobilities and the isotachopherogram in the steady state condition can be exactly simulated at a given electrolyte condition. When some of the necessary constants are unknown, they can be evaluated by means of a leastsquares method on the basis of the observed qualitative indices. Since the sample amount necessary for electrophoretic separation is very small and good separability from coexisting substances is expected, this may be one of the useful methods for determining the physico-chemical constants. When the necessary constants are known, the optimum separation condition can be obtained by an iterative simulation that varies the electrolyte condition, such as the electrolyte pH, the solvent (water and methanol) and the concentration of the complex-forming agents to give the largest effective mobility differences among the separands.

One‐dimensional simulation of lanthanide isotachophoresis using COMSOL

“…Computers can be used to solve the mathematical model for a variety of electrokinetic separation techniques and experimental system conditions. Hirokawa et al developed a model that uses an iterative scheme to vary the pH of each analyte zone in ITP and determine its concentration and effective mobility [27,33,34]. However, this model is limited to cases where the ITP has reached a steady state.…”

“…Hirokawa et al. developed a model that uses an iterative scheme to vary the pH of each analyte zone in ITP and determine its concentration and effective mobility []. However, this model is limited to cases where the ITP has reached a steady state.…”

Simulation and Verification of the Direct Current Electric Field on Fabricating High Porosity f-MWCNTs Thin Films by Electrophoretic Deposition Technique

“…Simulation in the EPD process allows us to better understand the electromigration process and determine the optimal deposition conditions without conducting experiments. Many works have explored the simulation of EPD process thus far. − These studies have contributed to the understanding of the processes of zone electrophoresis, − moving boundary electrophoresis, , isotachophoresis, − isoelectric focusing, − and isoelectric trapping . However, no specific studies have clearly shown the effect of the electric field on the thin film structure formed through cathodic electrophoretic deposition of f-MWCNTs.…”