Computer screen photo-assisted detection of complementary DNA strands using a luminescent zwitterionic polythiophene derivative

Monolithic SU-8 microcavities for efficient fluorescence collection

“…As put forward in 14 the majority of fluorescent light from a dipole will be emitted into the dielectric with the higher refractive index, at angles above the critical angle of the dielectric 15,16,17 . This poses a problem in that light emitted above the critical angle of a substrate will remain confined within the substrate.…”

Computer screen photo-assisted techniques for global monitoring of environmental and sanitary parameters

“…CSPT has been demonstrated for a number of applications during the past 4 years, among them the evaluation of bioassays such as enzyme linked immunosorbent assays (ELISA test for anti-neutrophil cytoplasmatic antibodies (ANCA) present in inflammatory diseases [41]), cell viability tests using MTT assays [16], pigment particle containing cell assays with transfected human ␤2 adrenergic receptors for the identification of adrenaline containing drugs [42], DNA strand recognition using fluorescent polythiophene derivatives [43], and ellipsometric non-labeled protein detection [44].…”

“…Diverse assays formats have been used with essentially the same instrumentation, from 96-well microtiterplates [16,33,[41][42][43], to cuvettes [16,45], microwells [46], and spotted glass slides [18].…”

“…Depending on the application, different optical properties have been evaluated, such as transmittance [16,22,32], reflectance [47][48][49], fluorescence [33,43,45,46,18] and polarization [34,44]. The way that the light source is operated becomes defined by each particular problem, for instance, kinetic measurements of transmittance can be done at an optimized illuminating color that reflects the largest changes in transmittance upon reaction with target analytes [32,41,42].…”

“…From the different modes of CSPT operation, a case of particular interest is the spectral characterization of fluorescent substances [18,33,43,45]. Recent progress in this area has shown that handy and inexpensive assays, still capable of sophisticated determinations, can be implemented when aiding CSPT with the right chemistry [18,52].…”