Computer programs to facilitate the estimation of time-dependent drug effects on ion channels

Marrannes, Roger; Prins, E. De

doi:10.1016/s0169-2607(03)00081-6

Cited by 9 publications

(6 citation statements)

References 7 publications

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“…Currents were sampled at 1 kHz and filtered at 333 Hz. Data acquisition and analysis were done with Pulse + Pulsefit (HEKA electronik, Lambrecht, Germany), Igor Pro [28] (Wavemetrics Inc., Lake Oswego, OR, USA), Prism and Instat Demo version (Graphpad Software Inc., San Diego, CA, USA; free at www.graphpad.com) software. Data were shown as mean ± standard error to the mean.…”

Section: Electrophysiologymentioning

confidence: 99%

P2X currents in peritoneal macrophages of wild type and P2X4−/− mice

Brône¹,

Moechars²,

Marrannes³

et al. 2007

Immunology Letters

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Section: Electrophysiologymentioning

confidence: 99%

P2X currents in peritoneal macrophages of wild type and P2X4−/− mice

Brône¹,

Moechars²,

Marrannes³

et al. 2007

Immunology Letters

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“…Current signals were amplified and digitized by an EPC-9 patch clamp amplifier, stored, and analyzed with Pulse/Pulsefit software (HEKA), DataAccess (Bruxton), and Igor Pro (Wavemetrics). 10 In all experiments, 87% to 90% series resistance compensation was achieved. After establishing the whole-cell configuration and waiting 5 min for equilibration, the stimulation protocol was continuously applied (identical double-step protocol as that described above) every 15 s, and rundown was corrected.…”

Section: Beerse Protocolmentioning

confidence: 99%

“…After establishing the whole-cell configuration and waiting 5 min for equilibration, the stimulation protocol was continuously applied (identical double-step protocol as that described above) every 15 s, and rundown was corrected. 10 Cells were continuously perfused by either control or drug-containing extracellular solution. The effect of compound was evaluated after 5 min of drug application.…”

Section: Beerse Protocolmentioning

confidence: 99%

Differential Inhibition of Inducible T Cell Cytokine Secretion by Potent Iron Chelators

et al. 2005

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The authors used the PatchXpress ® 7000A system to measure compound activity at the hERG channel using procedures that mimicked the "gold-standard" conventional whole-cell patch clamp. A set of 70 compounds, including hERG antagonists with potencies spanning 3 orders of magnitude, were tested on hERG302-HEK cells using protocols aimed at either identifying compound activity at a single concentration or obtaining compound potency from a cumulative concentration dependence paradigm. After exposure to compounds and subsequent washout of the wells to determine reversibility of the block, blockade by a reference compound served as a quality control. Electrical parameters and voltage dependence were similar to those obtained using a conventional whole-cell patch clamp. Rank order of compound potency was also comparable to that determined by conventional methods. One exception was flunarizine, a particularly lipophilic compound. The PatchXpress ® accurately identified the activity of 29 moderately potent antagonists, which only weakly displace radiolabeled astemizole and are false negatives in the binding assay. Finally, no false hits were observed from a collection of relatively inactive compounds. High-quality data acquisition by PatchXpress ® should help accelerate secondary screening for ion channel modulators and the drug discovery process. (Journal of Biomolecular Screening 2005:168-181)

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“…Some 3rd-party analysis packages are able to import pClamp files directly for analysis of data recorded in pClamp 9 suite, such as Origin, SigmaPlot [6], and DataView [7]. Marrannes and Prins [8] also designed IVOC and IROC programs to facilitate the analysis of drug effects on currents through ion channels in the cell membrane measured by means of voltage clamp. The programs are written in Igor Pro (WaveMetrics) and read exported files or raw data files generated by the PULSE (HEKA Electronic) or pClamp (Axon Instruments) systems.…”

Section: Introductionmentioning

confidence: 99%

“…Differing from some earlier studies such as IVOC and IROC [8], which programmed in Igor Pro, we designed a novel class module to directly read the raw data files recorded from HEKA instruments without any other program support (such as Igor Pro and Origin). We have preprocessed the raw data by using PCDReader in our previous patch clamp experiments, such as [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Desktop Software for Patch-Clamp Raw Binary Data Conversion and Preprocessing

Zhang

Tian

Yang

et al. 2011

Journal of Electrical and Computer Engineering

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Since raw data recorded by patch-clamp systems are always stored in binary format, electrophysiologists may experience difficulties with patch clamp data preprocessing especially when they want to analyze by custom-designed algorithms. In this study, we present desktop software, called PCDReader, which could be an effective and convenient solution for patch clamp data preprocessing for daily laboratory use. We designed a novel class module, called clsPulseData, to directly read the raw data along with the parameters recorded from HEKA instruments without any other program support. By a graphical user interface, raw binary data files can be converted into several kinds of ASCII text files for further analysis, with several preprocessing options. And the parameters can also be viewed, modified and exported into ASCII files by a user-friendly Explorer style window. The real-time data loading technique and optimized memory management programming makes PCDReader a fast and efficient tool. The compiled software along with the source code of the clsPulseData class module is freely available to academic and nonprofit users.

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Computer programs to facilitate the estimation of time-dependent drug effects on ion channels

Cited by 9 publications

References 7 publications

P2X currents in peritoneal macrophages of wild type and P2X4−/− mice

P2X currents in peritoneal macrophages of wild type and P2X4−/− mice

Differential Inhibition of Inducible T Cell Cytokine Secretion by Potent Iron Chelators

Desktop Software for Patch-Clamp Raw Binary Data Conversion and Preprocessing

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