Computer numerical control-printed paper electrodes for electrochemical detection of Pseudomonas aeruginosa virulence factor pyocyanin

Manisha, H.; Sonia, J.; Shashikiran, S.; Yuvarajan, S.; Rekha, Punchappady Devasya; Prasad, K. Sudhakara

doi:10.1016/j.elecom.2022.107259

Cited by 14 publications

(13 citation statements)

References 24 publications

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“…Its excellent electrochemical activity, due to its substituted phenazine structure, makes the toxin especially applicable for detection using cyclic voltammetry (CV) [126]. The electrochemical signals produced may also be amplified using redox activation or cycling, which would enable ultrasensitive detection of diagnostic biomarkers [127].…”

Section: Detection Methodsmentioning

confidence: 99%

Innovations in point-of-care electrochemical detection of pyocyanin

McLean

Brown

Windmill

et al. 2022

Journal of Electroanalytical Chemistry

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Section: Detection Methodsmentioning

confidence: 99%

Innovations in point-of-care electrochemical detection of pyocyanin

McLean

Brown

Windmill

et al. 2022

Journal of Electroanalytical Chemistry

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“…The fabrication of the paper spot array was carried out as per the previously reported method [34,35]. In brief, paper spot array designs were optimized using the Adobe Illustrator tool first, and then a photomask was created based on the requirements.…”

Section: Fabrication Of Paper Spot Arraysmentioning

confidence: 99%

A simple naked eye detection of Pb²⁺ on a gold nanocluster decorated disposable paper spot arrays

Kumara,

Naveen,

Sonia

et al. 2024

Phys. Scr.

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: Among all the heavy metal ions, Lead (Pb2+) has become a serious threat to human health and the environment. It is vital to develop an accurate system to monitor the Pb2+ level. Fluorescent and colorimetric sensors are one such system to detect Pb2+ with high accuracy. Herein, we used thiolated gold nanoclusters (GNC) decorated paper spot arrays for the detection of Pb2+ through a simple colorimetric method. The prepared GNC and the developed complex were well-studied using various characterizations. The addition of Pb2+ to GNC results in a slight shift in the absorption spectra from 485 nm to 534 nm. Further, GNC showed a broad emission peak centered around 650 nm, and the addition of Pb2+ resulted in an enhancement in the peak intensity, due to cation-induced-aggregation-induced emission enhancement (AIEE). The HR-TEM analysis confirms the spherical shape of the prepared GNCs with an average size of 2.60 nm. Whereas, the addition of Pb2+ onto GNC leads to the formation of an aggregated structure of size 6.17 nm due to the formation of a chelation complex. The GNC showed a particle size of 2 nm with a zeta potential of -16.45 mV, whereas the Pb2+@GNC complex exhibited an increment in an average size to 96 nm having a zeta potential of -19.67 mV, substantially demonstrating the capturing of Pb2+ by GNC. Overall, spectroscopic and surface morphology studies indicated aggregated induced emission (AIE) phenomena between GNC and Pb2+. The selective detection of Pb2+ by GNC was observed by the appearance of salmon pink to mauves colour and was captured using a smartphone. The developed paper spot array can detect up to 10 ppm of Pb2+. Further, the developed probe showed a distinguishable color change for Pb2+ along with other cations of interest such as Hg2+, Mg2+, K+, Mn2+, and Fe2+. Therefore, the present paper spot array could detect Pb2+ even in the presence of other metal ions due to its evident distinguishability. The fabricated GNC-embedded paper spot array obeys the new REASSURED criteria proposed by the World Health Organization (WHO) and could be used for on-spot detection of Pb2+.

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“…Research has been conducted on bacterial detection for food and environmental control using various methods for different applications [187][188][189]. S. aureus [143,190,191], S. typhimurium [144,192,193], Microcystin-LR [145,194], E. coli [195][196][197], L. monocytogenes [198,199] and P. aeruginosa [200][201][202] are popular pathogens that can be fatal to humans and animals. Bhardwaj et al [143] developed a labelfree µPAD EI for the detection of S. aureus using an antibody-(Ab-) single-walled carbon nanotube (SWCNT) bio-conjugate with DPV.…”

Section: µPed Immunosensors For Bacteriamentioning

confidence: 99%

Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

du Plooy,

Jahed,

Iwuoha

et al. 2023

R. Soc. open sci.

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Cellulose paper-based sensing devices have shown promise in addressing the accuracy, sensitivity, selectivity, analysis time and cost of current disease diagnostic tools owing to their excellent physical and physiochemical properties, high surface-area-to-volume ratio, strong adsorption capabilities, ease of chemical functionalization for immobilization, biodegradability, biocompatibility and liquid transport by simple capillary action. This review provides a comprehensive overview of recent advancements in the field of electrochemical immunosensing for various diseases, particularly in underdeveloped regions and globally. It highlights the significant progress in fabrication techniques, fluid control, signal transduction and paper substrates, shedding light on their respective advantages and disadvantages. The primary objective of this review article is to compile recent advances in the field of electrochemical immunosensing for the early detection of diseases prevalent in underdeveloped regions and globally, including cancer biomarkers, bacteria, proteins and viruses. Herein, the critical need for new, simplistic early detection strategies to combat future disease outbreaks and prevent global pandemics is addressed. Moreover, recent advancements in fabrication techniques, including lithography, printing and electrodeposition as well as device orientation, substrate type and electrode modification, have highlighted their potential for enhancing sensitivity and accuracy.

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Computer numerical control-printed paper electrodes for electrochemical detection of Pseudomonas aeruginosa virulence factor pyocyanin

Cited by 14 publications

References 24 publications

Innovations in point-of-care electrochemical detection of pyocyanin

Innovations in point-of-care electrochemical detection of pyocyanin

A simple naked eye detection of Pb²⁺ on a gold nanocluster decorated disposable paper spot arrays

Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

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Computer numerical control-printed paper electrodes for electrochemical detection of Pseudomonas aeruginosa virulence factor pyocyanin

Cited by 14 publications

References 24 publications

Innovations in point-of-care electrochemical detection of pyocyanin

Innovations in point-of-care electrochemical detection of pyocyanin

A simple naked eye detection of Pb2+ on a gold nanocluster decorated disposable paper spot arrays

Advances in paper-based electrochemical immunosensors: review of fabrication strategies and biomedical applications

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A simple naked eye detection of Pb²⁺ on a gold nanocluster decorated disposable paper spot arrays