Computer modeling of solution X-ray scattering intensity for biomacromolecules

Tong, Dudu

doi:10.32657/10356/69225

Cited by 2 publications

(3 citation statements)

References 104 publications

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“…This behavior is consistent with hMSCs cultured in other hydrogel systems with cell-remodelable adhesive ligands. [43][44][45] Orthogonal Functionalization of DA Cross-linked Hydrogels.…”

Section: Resultsmentioning

confidence: 99%

Rapid Diels–Alder Cross-linking of Cell Encapsulating Hydrogels

Madl

Heilshorn

2019

Chem. Mater.

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Recent efforts in the design of hydrogel biomaterials have focused on better mimicking the native cellular microenvironment to direct cell fate. To simultaneously control multiple material parameters, several orthogonal chemistries may be needed. However, present strategies to prepare cell-encapsulating hydrogels make use of relatively few chemical reactions. To expand this chemical toolkit, we report the preparation of hydrogels based on a Diels-Alder reaction between fulvenes and maleimides with markedly improved gelation kinetics and hydrolytic stability. Fulvene-maleimide gels cross-link up to 10-times faster than other commonly used DA reaction pairs and remain stable for months under physiological conditions. Furthermore, fulvenemaleimide gels presenting relevant biochemical cues, such as cell-adhesive ligands and proteolytic degradability, support the culture of human mesenchymal stromal cells. Finally, this rapid DA reaction was combined with an orthogonal click reaction to demonstrate how the use of selective chemistries can provide new avenues to incorporate multiple functionalities in hydrogel materials.

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“…This behavior is consistent with hMSCs cultured in other hydrogel systems with cell-remodelable adhesive ligands. [43][44][45] Orthogonal Functionalization of DA Cross-linked Hydrogels.…”

Section: Resultsmentioning

confidence: 99%

Rapid Diels–Alder Cross-linking of Cell Encapsulating Hydrogels

Madl

Heilshorn

2019

Chem. Mater.

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“…The use of a support matrix inside a hollow tube has been shown previously to help improve the likelihood of nerves bridging a transection injury. 66,67 A variety of different naturally derived materials have previously been used as luminal fillers to promote peripheral nerve repair in guidance conduits. [12][13][14][15][16][17] For example, Chen et al used a similar experimental design as the one presented here, and they observed that 100% of silicone conduits filled with collagen, laminin, and fibronectin mixtures regenerated nerves across a 10 mm gap compared to only 60% of empty silicone conduit groups at six-weeks post implantation.…”

Section: Discussionmentioning

confidence: 99%

“…83 The animal model selected here (a 10 mm gap over 6 weeks) was selected to be consistent with other previously published work. 66,67 While this shorter time-point is ideal for tissue-bridging studies, functional regeneration in PNI models, even with the "gold standard" autograft, can take upward of months to a year to begin approaching preinjury levels (SFI: −70 to −60). 61,62,84 Consistent with these previous reports, we observed that peripheral nerve function values were all much lower at 6 weeks than the preinjured state (Figures 3, S3, and S4).…”

Section: Discussionmentioning

confidence: 99%

Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits

Suhar

Marquardt

Song

et al. 2020

ACS Biomater. Sci. Eng.

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Synthetic nerve guidance conduits (NGCs) offer an alternative to harvested nerve grafts for treating peripheral nerve injury (PNI). NGCs have been made from both naturally derived and synthesized materials. While naturally derived materials typically have an increased capacity for bioactivity, synthesized materials have better material control, including tunability and reproducibility. Protein engineering is an alternative strategy that can bridge the benefits of these two classes of materials by designing cell-responsive materials that are also systematically tunable and consistent. Here, we tested a recombinantly derived elastin-like protein (ELP) hydrogel as an intraluminal filler in a rat sciatic nerve injury model. We demonstrated that ELPs enhance the probability of forming a tissue bridge between the proximal and distal nerve stumps compared to an empty silicone conduit across the length of a 10 mm nerve gap. These tissue bridges have evidence of myelinated axons, and electrophysiology demonstrated that regenerated axons innervated distal muscle groups. Animals implanted with an ELP-filled conduit had statistically higher functional control at 6 weeks than those that had received an empty silicone conduit, as evaluated by the sciatic functional index. Taken together, our data support the conclusion that ELPs support peripheral nerve regeneration in acute complete transection injuries when used as an intraluminal filler. These results support the further study of protein engineered recombinant ELP hydrogels as a reproducible, off-the-shelf alternative for regeneration of peripheral nerves.

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Computer modeling of solution X-ray scattering intensity for biomacromolecules

Cited by 2 publications

References 104 publications

Rapid Diels–Alder Cross-linking of Cell Encapsulating Hydrogels

Rapid Diels–Alder Cross-linking of Cell Encapsulating Hydrogels

Elastin-like Proteins to Support Peripheral Nerve Regeneration in Guidance Conduits

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