“…High performance liquid chromatographic (HPLC) methods for separation and quantitation of the steviol glycosides on bonded phase columns are most usually employed currently. Separation has been performed either on columns with terminal -NH2 or -OH groups (Hashimoto and Moriyasu, 1978;Makapugay et al, 1984), or on columns specially designed for separation of carbohydrates (Chen and Yeh, 1978) or proteins (Ahmed and Dobberstein, 1982a, b;Dobberstein and Ahmed, 1982). Reversed-phase HPLC on octadecyl columns was successfully applied also (Gagliardi er af., 1986, Alvarez andKusumoto, 1987;Kitada et al, 1989).…”