“…In association, it should also be considered that in addition to the increase in metabolic activity and sperm degeneration (Gangwar et al, 2020) these gametes produce ROS under aerobic conditions as a function of their metabolism, contributing to the installation of oxidative stress by favoring pro-oxidant agents over those antioxidants (Lenzi et al, 2002;Piomboni et al, 2012;Gangwar et al, 2015;Tariq et al, 2015). Thus, as the number of dead sperms increases, oxidative stress is intensified, causing the oxidation of essential biomolecules such as proteins and lipids (Tariq et al, 2015;Rajashri et al, 2017). A contrary result was observed for membrane integrity in the 720h period (Fig.…”