Computer assisted scanning laser monitor of optical quality of the excised crystalline lens

Sivak, J. G.; Gershon, David; Dovrat, A.; Weerheim, J.

doi:10.1016/0042-6989(86)90139-2

Cited by 66 publications

(19 citation statements)

References 11 publications

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“…Lens optical monitoring was done by an automated laser scanning system that records focal length according to Sivak et al 32 A low power heliumneon laser mounted on a computer driven X-Y table with two video cameras and a video digitizer was used. The laser was programmed to scan across the lens in the axial direction in small steps.…”

Section: In Vivo Experimentsmentioning

confidence: 99%

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Featured Article: Inhibition of diabetic cataract by glucose tolerance factor extracted from yeast

Mirsky

Cohen

Eliaz

et al. 2016

Exp Biol Med (Maywood)

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Diabetes leads to many complications; among them is the development of cataract. Hyperglycemia brings to increased polyol concentration in the lens, to glycation of lens proteins, and to elevated level of ROS (Reactive Oxygen Species) causing oxidative stress. The glucose tolerance factor (GTF) was found by several groups to decrease hyperglycemia and oxidative stress both in diabetic animals and humans. The aim of our study was to explore the damages induced by high glucose to the eye lens and to assess the protective effects of GTF both in vivo and in vitro. The in vivo study included control healthy rats, streptozotocin (STZ) diabetic untreated rats, and STZ diabetic rats orally treated with 15 doses of GTF. The diabetic untreated rats developed cataracts, whereas the development of cataract was totally or partially prevented in GTF treated animals. In vitro studies were done on bovine lenses incubated for 14 days. Half of the lenses were incubated in normal glucose conditions, and half in high glucose conditions (450 mg%). To one group of the normal or high glucose condition GTF was added. The optical quality of all the lenses was measured daily by an automated scanning laser system. The control lenses, whether with or without GTF addition, did not show any reduction in their quality. High glucose conditions induced optical damage to the lenses. Addition of GTF to high glucose conditions prevented this damage. High glucose conditions affected the activity of aldose reductase and sodium potassium ATPase in lens epithelial cell. Addition of GTF decreased the destructive changes induced by high glucose conditions. The amount of soluble cortical lens proteins was decreased and structural changes were detected in lenses incubated in high glucose medium. These changes could be prevented when GTF was added to high glucose medium. Our findings demonstrate the anticataractogenic potential of GTF.

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Section: In Vivo Experimentsmentioning

confidence: 99%

“…31 We established a method for monitoring lens optical quality during culture period. 32 This method can be used to detect early damage in the eye lens: focal changes appear when the lens is still clear.…”

Section: Introductionmentioning

confidence: 99%

Featured Article: Inhibition of diabetic cataract by glucose tolerance factor extracted from yeast

Mirsky

Cohen

Eliaz

et al. 2016

Exp Biol Med (Maywood)

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“…Laser scanning is commonly used to determine the optical properties of teleost lenses (Kröger et al 1994;Malkki and Kröger 2005;Sivak et al 1986). Four chambers were mounted on a motorized linear stage (Linos x.act LT 200-2 ST) so that each chamber could be moved into the measuring position.…”

Section: Laser Scanningmentioning

confidence: 99%

“…Direct photography and subsequent image analysis for determination of affected areas have previously been used (Zhou and Menko 2004). Other methods are for instance those used by Hightower and Dering where the opacity of the lens was measured by determining the reduction in light intensity after the light beam had passed through the lens (Hightower and Dering 1984), and the optical method for studying the quality of the lens during culturing (Sivak et al 1986). The latter method, although yielding results with highresolution, predominantly measures the center of the lens, leaving changes occurring in the lens periphery unaddressed.…”

Section: Introductionmentioning

confidence: 99%

Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements

2010

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Culturing whole lenses is a frequently used method for studying regulatory events on the lens in controlled environments. The evaluation methods used often fall under two categories, molecular or optical. The main benefit from optical measurements is that they directly detect changes in the lens' main function, i.e. refracting light. However, these measurements often have rather low resolution or yield results open for subjective interpretation. Here we present a short-term crystalline lens culturing technique combined with a high-resolution optical measuring method. There are two main advantages of using teleost lenses compared to mammalian lenses. Teleost tissue generally has a higher tolerance than mammalian tissue with regard to temperature and nutrient fluctuations. Teleost lenses are structurally more robust and can be excised from the eye without disturbing form or function. The technique is developed for short-term culturing (3 h), however, the lenses appear viable for at least 24 h and longer culturing may be possible. The technique is resistant to small variations in osmolarity and yields quantitative datasets for further analyses and statistical treatment.

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“…Harnessing expertise developed Sivak et al, 1986] to characterize the optical properties of intact bovine lens in long-term culture conditions, we developed a system to determine the effect of microwave radiation on the eye lens. Lenses were excised from eyes of 1-year-old male calves, obtained from a slaughterhouse; the lenses were incubated in culture within 2-4 h. From each pair, one lens is exposed to microwave radiation and the lens from the other eye is used for control.…”

Section: Lens Organ Culture Systemmentioning

confidence: 99%

Localized effects of microwave radiation on the intact eye lens in culture conditions

Dovrat

Berenson

Bormusov

et al. 2005

Bioelectromagnetics

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A novel experimental system was used to investigate the localized effects of microwave radiation on bovine eye lenses in culture for over 2 weeks. Using this setup, we found clear evidence that this radiation has a significant impact on the eye lens. At the macroscopic level, it is demonstrated that exposure to a few mW at 1 GHz for over 36 h affects the optical function of the lens. Most importantly, self-recovery occurs if the exposure is interrupted. At the microscopic level, close examination of the lens indicates that the interaction mechanism is completely different from the mechanism-causing cataract via temperature increase. Contrary to the latter's effect, that is particularly pronounced in the vicinity of the sutures and it is assumed to be a result of local friction between the edges of the fibers consisting the lens. Even if macroscopically the lens has recovered from the irradiation, microscopically the indicators of radiation impact remain.

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Computer assisted scanning laser monitor of optical quality of the excised crystalline lens

Cited by 66 publications

References 11 publications

Featured Article: Inhibition of diabetic cataract by glucose tolerance factor extracted from yeast

Featured Article: Inhibition of diabetic cataract by glucose tolerance factor extracted from yeast

Short-term culturing of teleost crystalline lenses combined with high-resolution optical measurements

Localized effects of microwave radiation on the intact eye lens in culture conditions

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