Computer-assisted analysis of sperm motion in goats and its relationship with sperm migration in cervical mucus

Cox, J.F.; Alfaro, Verónica; Montenegro, V. A.; Rodriguez‐Martinez, Heriberto

doi:10.1016/j.theriogenology.2006.01.062

Cited by 54 publications

(38 citation statements)

References 24 publications

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“…No presente estudo, não foi analisado o perfil de ácidos graxos na membrana plasmática dos espermatozoides. No entanto, supõe-se que o aumento do BCF para os animais tratados indica a possibilidade de haver maior incorporação de UFAs na membrana espermática e, por isso, ter havido aumento da intensidade dos batimentos, como reportado para bovinos (GHOLAMI et al, 2010) Apesar da linearidade dos espermatozoides do grupo OP ter diminuído no Período IV, os níveis obtidos (63,7%) são semelhantes aos níveis mais adequados de linearidade para fertilização (COX et al, 2002), pois estudos realizados com sêmen caprino demonstram que espermatozoides com valor médio para LIN de 60±1,3% foram capazes de percorrer maiores distâncias no muco cervical in vitro (P<0,05) quando comparados aos ejaculados que possuíam 66,6±1,0% e 58±1,4% de linearidade no movimento espermático (COX et al, 2006). Sabe-se que a viscosidade do meio interfere na livre circulação dos espermatozoides pelo plasma seminal (CASTELLINI et al, 2000).…”

Section: Pi_h342 (%) E_n (%)unclassified

“…A análise computadorizada da motilidade espermática (CASA) calcula de forma objetiva a motilidade e os parâmetros de movimentação do sêmen, proporcionando maior acurácia à avaliação (VERSTEGEN; IGUER-OUADA; ONCLIN, 2002;MORTIMER, 2000;COX et al, 2006). Esta ferramenta não somente pode ser utilizada para diminuir ou retirar a subjetividade do método convencional de avaliação da motilidade, mas também para investigar a cinética detalhada dos espermatozoides (MANDAL; BAGPAUL; GUPTA, 2003).…”

Section: Introductionunclassified

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A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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This research aimed to evaluate the effect of inclusion unsaturated fatty acids (UFAs) in the buffalo diet, on the sperm quality after thawing. In parallel, it was verified the degree of association among techniques to assess plasma membrane integrity (PMI) of spermatozoa. Patterns of sperm motility (subjective and computer-assisted analysis), kinetics, morphology and plasma membrane integrity were evaluated. The hypo-osmotic swelling test, nigrosin-eosin staining (E-N) and fluorescent probes propidium iodide/Hoechst 33342 (IP-H342) were adopted to evaluate PMI. The study was planned considering two experimental groups, which were evaluated in repeated measures design. over time. Eleven Murrah buffalo bulls (3.5±1.0 years, and 536.9±73.0 kg) were divided in Control Group (CONT, n=5) and Palm Oil Group (PO, n=6). Animals were fed with forage and concentrated in a controlled manner. Animals of PO received dietary supplementation containing palm oil (2% of dry matter). Dietary consumption was daily monitored and the intake level of UFAs in PO was 55.4% higher than CONT. Semen samples were collected and evaluated every 14 days. The experiment lasted 120 days, considering four periods of 30 days each (Periods I, II, III and IV) and repeated measures structure was used in statistical analysis. Significance level adopted was 5%. There was no effect of treatment on sperm motility (P>0.05), but palm oil supplementation presented a positive effect on spermatic ability to progress (derived from BCF, STR and LIN) in Period IV (P<0.05). High correlation (91%) was observed between IP-H342 and E-N techniques (Y = 0.9155 x + 2.9843, R 2 = 0.82, P≤0.05). Thus, supplementation with UFAs increased the progressivity of spermatozoa after 90 days, and had no effect on other seminal parameters. Eosin-nigrosin stain can be used efficiently as a predictor of the level of PMI when conditions for implementing fluorescent probes not present.

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Section: Pi_h342 (%) E_n (%)unclassified

Section: Introductionunclassified

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

et al. 2014

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“…Analyses were carried out with logtransformed variables. Data for straightline velocity (VSL) were obtained from the literature (Mortimer and Mortimer, 1990;Stachecki et al, 1993Stachecki et al, , 1994Shivaji et al, 1995;Mahonynet al, 1996;Moore and Akhondi, 1996;Trimeche et al, 1996Trimeche et al, , 1999Patil et al, 1998;van der Horst et al, 1999;Castellini et al, 2000Castellini et al, , 2006Jayaprakash et al, 2001;Kawaguchi et al, 2004;Lavara et al, 2005;Miró et al, 2005;Baumber and Meyers, 2006;Cox et al, 2006;Sloter et al, 2006;Aurich et al, 2007;Grzmil et al, 2007;Holt et al, 2007;Hung et al, 2007;Immler et al, 2007a;Rijsselaere et al, 2007) and our own unpublished observations. Data for total sperm length were obtained from Cummins and Woodall (1985), Cassinello et al (1998), Gage and Freckelton (2003) and Malo et al (2006 Pitnick et al, 2001).…”

Section: ) (C)mentioning

confidence: 99%

Implications of diversity in sperm size and function for sperm competition and fertility

Gomendio

Roldán²

2008

Int. J. Dev. Biol.

167

185

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Sperm competition is now recognised as a potent selective force shaping many male reproductive traits. While the influence of sperm competition on sperm number is widely accepted, its effects upon sperm size remain controversial. It had been traditionally assumed that there is a trade-off between sperm number and sperm size, so that an increase in sperm number would result in a decrease in sperm size, under conditions of sperm competition. Contrary to this prediction, we proposed some time ago that sperm competition favours an increase in sperm size, because longer sperm swim faster and are more likely to win the race to fertilize ova. Comparative studies between species show that in many taxa such a relationship exists, but the consequences of an increase in sperm size may vary between taxa depending on the environment in which spermatozoa have to compete. We present new evidence showing that in mammals longer sperm swim at higher speeds. We also show that mean swimming speed is highly correlated with maximum swimming speed, so even if the fastest swimming sperm are more likely to fertilize, both measures are informative. When individuals of the same species are compared, ratios between the dimensions of different sperm components, as well as the shape of the head, seem better at explaining sperm swimming velocity. Finally, we show that mean and maximum sperm swimming speed determine male fertility. Other studies have shown that in competitive contexts, males with faster swimming sperm have higher fertilization success. We conclude that the available evidence supports our original hypothesis.

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“…Cox et al (2006) found that ejaculates with improved VCL, VAP and VSL had better spermatozoan migration efficiency in homologous cervical mucus. However, Furstoss et al (2010) used a combination of parametres to predict fertility, but, surprisingly, predicting in vivo fertility with using CASA parametres was less precise than results of traditional methods of semen evaluation.…”

Section: Buck Semenmentioning

confidence: 93%

Proper use and impact of ‘Computer Assisted Semen Analysis’ technique on semen evaluation of farm animals

Michos

Basioura

Boscos

et al. 2017

J Hellenic Vet Med Soc

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Objective of this review is to present the use of ‘Computer Assisted Semen Analysis’ technique application in farm animal health management, in particular the steps for accurate semen evaluation and the impact of the method in predicting male animal fertility under field conditions. Requirements for proper use of the equipment, factors affecting the evaluation process and the role of the estimated parametres for fertility under field conditions are described. Special reference is made in sperm motility evaluation. It is concluded that the method is an effective and efficient tool for semen evaluation, provided good practices are strictly applied and adhered to, by means of which valid results may be obtained.

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Computer-assisted analysis of sperm motion in goats and its relationship with sperm migration in cervical mucus

Cited by 54 publications

References 24 publications

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

A suplementação alimentar com ácidos graxos insaturados melhora a qualidade do sêmen criopreservado de búfalos (Bubalus bubalis) pós descongelação

Implications of diversity in sperm size and function for sperm competition and fertility

Proper use and impact of ‘Computer Assisted Semen Analysis’ technique on semen evaluation of farm animals

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