Computer-aided semen analysis: sperm concentration assessment by the Strömberg-Mika system

Togni, G; Gallicciotti, G.; Coccia, P.; Piffaretti-Yanez, A.; Stamm, J.; Balerna, M.

doi:10.1111/j.1439-0272.1995.tb02096.x

Cited by 9 publications

(5 citation statements)

References 22 publications

(18 reference statements)

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“…, 1999). This is consistent with another study who found that the only parameter to be positively correlated with a positive outcome was the degree of sperm motility following preparation (Togni et al. , 1995).…”

Section: Discussionsupporting

confidence: 92%

“…Pasqualotto et al (1999) did not found this relationship between the postwash motile sperm count, but showed that the post-wash motility predicted IUI success at a cut-off of 40% (Thyer et al, 1999). This is consistent with another study who found that the only parameter to be positively correlated with a positive outcome was the degree of sperm motility following preparation (Togni et al, 1995). Few studies include CASA parameters in their semen analysis.…”

Section: Discussionmentioning

confidence: 83%

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Predictive value of CASA parameters in IUI with frozen donor sperm

et al. 2009

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The objective of this study was to determine if characteristics of sperm motion determined by computer-aided semen analysis (CASA) after thawing and preparation on discontinuous gradient could predict pregnancy outcome after intrauterine insemination (IUI) from frozen donor sperm. A retrospective analysis of 100 non-selected women undergoing 171 consecutive donor insemination cycles was conducted between January 2006 and April 2007. Semen samples from all donors were analysed after thawing and density gradient preparation. Women who became pregnant and those who did not were comparable in terms of age, ovarian stimulation regimen and indication of IUI with donor semen. Pregnancy rate per cycle was 21.8%, and pregnancy occurred after 2.5 IUI cycles on average. Motility parameters of sperm measured by CASA (VAP, VCL, VSL, LIN, STR, and ALH) and total spermatozoa concentration after preparation on discontinuous gradient showed no difference in both groups. Progressive and total motile spermatozoa concentration, as well as progressive and total motile percentages was significantly higher in pregnancy group. The receiver operating characteristic (ROC) curve analysis showed that total motile percentage >17% and motile concentration >0.9 x 10(6)/mL best predicted pregnancy. In a multivariate analysis, only total motility percentage was able to predict pregnancy. Sperm motility parameters of frozen-thawed prepared donor sperm obtained by CASA do not seem to predict pregnancy in IUI cycles. Total motile and progressive percentages and concentrations remain the best prognostic elements for pregnancy in IUI with donor semen.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 83%

Predictive value of CASA parameters in IUI with frozen donor sperm

et al. 2009

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“…The low number of spermatozoa in these groups may have also contributed to some level of error in the manual assessment as well, despite strict fulfillment of WHO 5th recommendations in terms of sperm dilution. Several papers reported lower accuracy at very low sperm concentration levels using CASA systems [27][28][29]. In the severe oligozoospermic group, the CASA showed a much higher median for sperm concentration: Greater than 4 times the manual results (CASA: 7.6 vs. Manual: 1.75×10 6 / ml) whereas the SQA-V GOLD was in line with the manual results.…”

Section: Discussionmentioning

confidence: 93%

Double-blind prospective study comparing two automated sperm analyzers versus manual semen assessment

Lammers¹,

Splingart

Barrière

et al. 2013

J Assist Reprod Genet

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Purpose Despite controversy regarding its clinical value, male fertility investigation mainly relies on semen analysis. Even though reference guidelines are available, manual sperm analysis still suffers from analytical variability, thus questioning the interest of automated sperm analysis systems. The aim of this study is to compared automated computerized semen analysis systems (SQA-V GOLD and CASA CEROS) to the conventional manual method in terms of accuracy and precision. Methods We included 250 men in this double-blind prospective study. The SQA-V GOLD (Medical Electronic Systems) and CEROS, CASA system (Hamilton Thorne) were compared to the standard manual assessment based on the WHO 5th Edition. The main outcome measures were sperm concentration, total sperm number, total motility, progressive motility, non-progressive motility, morphology, motile sperm concentration (MSC) and progressively motile sperm concentration (PMSC) with the three methods. Results Statistical analysis of the test results from the automated systems and the manual method demonstrated no significant differences for most of the semen parameters. The Spearman coefficients of rank correlation (rho) for CASA and the SQA-V GOLD automated systems vs. the manual method were: Sperm concentration (0.95 and 0.95), total sperm number (0.95 and 0.95), MSC (0.94 and 0.96) and PMSC (0.94 and 0.93) correspondingly. Concerning sperm morphology, both automated systems demonstrated high specificity (Sp) and negative predictive values (NPV), despite significantly different medians (CASA: 83.7 % for Sp and 95.2 % for NPV, SQA-V: 97.9 % for Sp and 92.5 %). The highest precision (lowest 95 % confidence interval for duplicate tests) for all semen variables was found in the SQA-V GOLD. Conclusions The advantages of using automated semen analysers are: Standardization, speed (lower turnaround time), precision, reduced potential for human error, automated data recording and less need for highly skilled professionals to run the systems. The disadvantages of using automated systems are: notably the problem with testing some atypical samples and the inability to perform an assessment of morphology abnormalities. Based on the results of this study, the SQA-V Gold demonstrated better agreement vs. the manual method. In conclusion, automated semen analyzers can be used for routine semen analysis providing rapid clinically acceptable results with higher precision, and positively impacting laboratory standardization.

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“…Sperm analyses were carried out according to the WHO criteria (WHO, 1999). Semen samples were analysed within 1 h following ejaculation by using a computer-aided semen analyzer (Stro ¨mberg Mika-cell motility analyzer; Medical Technologies Montreux SA, Montreux, Switzerland) (Togni et al, 1995). If the sperm concentration was ,3 million/ml, standard microscopic investigation was performed.…”

Section: Sperm Analysismentioning

confidence: 99%