Computed tomography of pulmonary sarcoid-like granulomas induced by complete Freund's adjuvant in rats

Bergeron, Anne; Jp, Laissy; Loiseau, P; Schouman-Claeys, É.; Aj, Hance; Tazi, Abdellatif

doi:10.1183/09031936.01.00086001

Cited by 7 publications

(2 citation statements)

References 21 publications

(23 reference statements)

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“…CFA is a well-known adjuvant and itself can induce granuloma formation in the lung when emulsified CFA is administered intravenously [27,28]. Intravenous injection of CFA induced granulomas in the lungs of both OV and SHAM rats (fig.…”

Section: Resultsmentioning

confidence: 99%

Sex Hormones Alter Th1 Responses and Enhance Granuloma Formation in the Lung

Tajima

Miura²,

Ishiwata³

et al. 2011

Respiration

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Background: The lung is one of the sites of granulomatous responses, which are characterized by the recruitment and organization of activated macrophages and lymphocytes. There have been several reports that have shown that some pulmonary granulomatous diseases, such as sarcoidosis and nontuberculous mycobacterial disease, are likely to be characterized by a preponderance in postmenopausal females. Although sex hormones have been shown to play an important role in the regulation of the immune system, the influence of sex hormones on pulmonary granuloma formation is still unclear. Objectives: The purpose of this study was to assess whether sex hormones are involved in granulomatous inflammation and to evaluate how sex hormones modulate this response in the lung. Methods: Ovariectomized rats were used as an experimental postmenopausal model in which chronic pulmonary granulomatous inflammation was induced by intravenous injection of complete Freund’s adjuvant. Results: Histological analysis of lung tissues demonstrated enhancement of granuloma formation in the ovariectomized group. Such enhanced granuloma formation was significantly associated with generalized Th1-biased cytokine production in the bronchoalveolar lavage fluid. Conclusion: These results indicate that sex hormones play an important role in pulmonary granuloma formation by altering the Th1 responses.

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Section: Resultsmentioning

confidence: 99%

Sex Hormones Alter Th1 Responses and Enhance Granuloma Formation in the Lung

Tajima

Miura²,

Ishiwata³

et al. 2011

Respiration

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“…Complete Freund's adjuvant (CFA) was also used to establish an animal model of sarcoidosis. Bergeron et al (17) injected CFA into the tail vein of Wistar rats to develop a rat model of granulomatosis. Since the mechanism of action of CFA-induced granulomatosis is unclear, the CFA injection method is not commonly adopted.…”

Section: Discussionmentioning

confidence: 99%

IL-17A Can Promote Propionibacterium acnes-Induced Sarcoidosis-Like Granulomatosis in Mice

Song

Zhao

et al. 2019

Front. Immunol.

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The etiology of sarcoidosis is unknown. In this study, Propionibacterium acnes (PA) was used to induce sarcoidosis-like granulomatous inflammation in a mouse model. Wild-Type (WT) C57BL/6 mice were divided into three groups: (1) WT-PA group; (2) WT-PA + Incomplete Freund's Adjuvant (IFA) group; and (3) WT-PBS group. Loose granuloma formation was observed in the lungs on day 56 in the WT-PA and WT-PA + IFA groups. The proportions of peripheral Th17 cells in the WT-PA ( p = 0.0004) and WT-PA + IFA groups ( p = 0.0005) were significantly higher than that in the WT-PBS group. The proportions of peripheral Treg cells in the WT-PA ( p < 0.0001) and WT-PA + IFA groups ( p < 0.0001) were lower than that in the WT-PBS group. Then, to explore the mechanism of IL-17, Wild-Type (WT) C57BL/6 mice were divided into three groups: (1) WT-PBS group (2) WT-PA group; (3) WT-PA + mouse IL-17A neutralizing antibody (IL-17Ab) group. IL-17A gene knockout mice (KO) were divided into two groups: (1) KO -PA group; (2) KO-PBS group. The KO-PA and WT-PA + IL-17Ab groups showed reduced inflammation and no loose granuloma formation on day 56. As compared to the WT-PA group, the ratio of peripheral Th17 in the KO-PA ( p < 0.0001) and WT-PA + IL-17Ab groups ( p < 0.0001) decreased, while the ratio of peripheral Treg in the KO-PA ( p < 0.0001) and WT-PA + IL-17Ab ( p = 0.0069) groups increased on day 56. Hence, PA can be used to establish a mouse model of sarcoidosis-like granuloma. IL-17A plays an important role in experimental sarcoidosis-like granuloma formation.

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Lung Tissue Mitochondrial Benzodiazepine Receptors Increase in a Model of Pulmonary Inflammation

Audi

Dawson

Ahlf

et al. 2002

Lung

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Pulmonary inflammation induced in the rabbit lung by the intravenous injection of complete Freund's adjuvant (CFA) increases the lung uptake of 14C-diazepam from the pulmonary circulation. The objective of this study was to determine the extent to which mitochondrial (or peripheral) benzodiazepine receptors (mBRs) may contribute to this increased uptake. To this end, we measured the pulmonary venous effluent concentration versus time for 14C-diazepam following its injection into the pulmonary artery of isolated perfused normal and CFA inflamed lungs with and without an inhibitor (PK11195) of diazepam binding to mBRs. The results demonstrate that this model of pulmonary inflammation is associated with an increase in lung tissue mBR. Lung tissue caspase-3 activity was also measured as one index of lung inflammation, and we found that in inflamed lungs, there was an inverse correlation between mBR density and lung tissue capase-3 activity. This is consistent with observations in other organs and a role for mBRs in apoptotic elimination of inflammatory cells in the resolution of this inflammatory response. The results suggest the potential utility of mBR ligands for noninvasive detection and/or characterization of pulmonary inflammation, e.g., via nuclear medicine methods.

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Computed tomography of pulmonary sarcoid-like granulomas induced by complete Freund's adjuvant in rats

Cited by 7 publications

References 21 publications

Sex Hormones Alter Th1 Responses and Enhance Granuloma Formation in the Lung

Sex Hormones Alter Th1 Responses and Enhance Granuloma Formation in the Lung

IL-17A Can Promote Propionibacterium acnes-Induced Sarcoidosis-Like Granulomatosis in Mice

Lung Tissue Mitochondrial Benzodiazepine Receptors Increase in a Model of Pulmonary Inflammation

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