17Sentinel lymph node (SLN) mapping is common in many types of human cancers, and is 18 gaining utility in veterinary medicine. There are currently many different methods described in 19 veterinary medicine for pre-operative SLN mapping, however, most of these are restricted to 20 referral institutions due to cost and need for specialized equipment. The purpose of this 21 prospective, pilot study was to evaluate the feasibility of radiographic evaluation of water-22 soluble, iodinated contrast (WIC) injected subcutaneously for lymphography in dogs. Eight dogs 23 were injected with 1-2 milliliters of WIC into the subcutaneous tissues overlying the tarsus in 4 24 separate locations mimicking a circumferential, peri-tumoral injection. Radiographs were taken 25 at select time points up to 50 minutes. Image sequences were evaluated by a single, board-26 certified radiologist. All 8 dogs had visible contrast-enhancing lymphatic channels. Median time 27 to lymphatic enhancement was immediately post-injection. Seven dogs (88%) had 8 contrast 28 enhancing lymph nodes (7 popliteal and 1 superficial inguinal). Median time to lymph node 29 enhancement was 20 minutes. In this study, the plantar aspect of the pes drained to the 30 superficial inguinal lymph node, and the dorsal aspect of the pes drained to the popliteal lymph 31 node. Subcutaneously-injected WIC was readily identifiable in the lymphatic channels and 32 draining lymph node(s). Subcutaneously injected WIC may offer a practical alternative to 33 previously described pre-operative methods of SLN mapping. Additionally, one cannot assume 34 that the popliteal lymph node alone, drains the distal pelvic limb.35