Computational Tools: RNA Interference in Fungal Therapeutics

Jain, Chakresh Kumar; Wadhwa, Gulshan

doi:10.1007/978-981-10-7483-7_12

Cited by 2 publications

(1 citation statement)

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“…In addition to hairpin RNAs ( Drinnenberg et al, 2009 ; Crook et al, 2014 ; Purcell et al, 2018 ), other forms of RNAi precursors, such as dsRNAs ( Si et al, 2014 ; Crook et al, 2016 ) and antisense RNAs ( Suk et al, 2011 ; Si et al, 2014 , 2017 ), can also be accepted by the S. castellii RNA machinery for gene repression in S. cerevisiae . Notably, although a growing collection of computational tools is available to design RNAi targeting sequences, these tools are primarily developed for canonical RNAi pathways as recently summarized ( Lagana et al, 2014 ; Jain and Wadhwa, 2018 ). Given the mechanistic differences of siRNA generation between canonical and budding-yeast RNAi machinery, it remains elusive if existing algorithms may help to identify RNAi targets for efficient gene silencing in S. cerevisiae .…”

Section: Tool Developmentmentioning

confidence: 99%

Advances in RNAi-Assisted Strain Engineering in Saccharomyces cerevisiae

Chen

Guo

Zhang

et al. 2020

Front. Bioeng. Biotechnol.

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Saccharomyces cerevisiae is a widely used eukaryotic model and microbial cell factory. RNA interference (RNAi) is a conserved regulatory mechanism among eukaryotes but absent from S. cerevisiae . Recent reconstitution of RNAi machinery in S. cerevisiae enables the use of this powerful tool for strain engineering. Here we first discuss the introduction of heterologous RNAi pathways in S. cerevisiae , and the design of various expression cassettes of RNAi precursor reagents for tunable, dynamic, and genome-wide regulation. We then summarize notable examples of RNAi-assisted functional genomics and metabolic engineering studies in S. cerevisiae . We conclude with the future challenges and opportunities of RNAi-based approaches, as well as the potential of other regulatory RNAs in advancing yeast engineering.

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