Computational tests of a thermal cycling strategy to isolate more complex functional nucleic acid motifs from random sequence pools by in vitro selection
Abstract:The dual information-function nature of nucleic acids has been exploited in the laboratory to isolate novel receptors and catalysts from random DNA and RNA sequences by cycles of in vitro selection and amplification. This strategy is particularly effective because, unlike polypeptides with random amino acid sequences, nucleic acids with random base sequences are often capable of stably folding into defined three-dimensional structures. However, the pervasive base-pairing potential of nucleic acids is also know… Show more
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