Computational stabilization of a non-heme iron enzyme enables efficient evolution of new function
Brianne R. King,
Kiera H. Sumida,
Jessica L. Caruso
et al.
Abstract:Directed evolution has emerged as a powerful tool for engineering new biocatalysts. However, introducing new catalytic residues can be destabilizing, and it is generally beneficial to start with a stable enzyme parent. Here we show that the deep learning tool ProteinMPNN can be used to redesign an Fe(II)/alphaKG superfamily enzyme for greater stability, solubility, and expression while retaining both native activity and an industrially-relevant non-native function. We performed site-saturation mutagenesis with… Show more
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