Abstract:Ketosteroid Isomerase (KSI) performs an important biological function in the metabolism of many bacteria by degrading steroids. Pseudomonas putida KSI (PpKSI) is strikingly efficient and selective. Three putative KSIs identified from Structural Genomics were analyzed by THEMATICS (THEoretical Microscopic Anomalous TItration CurveS) and POOL (Partial Order Optimum Likelihood) and then characterized in vitro to determine their KSI activity. A putative KSI from Mycobacterium tuberculosis (MtKSI) was predicted to … Show more
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