Computational models of hair cell bundle mechanics: III. 3-D utricular bundles

Silber, Joseph; Cotton, John R.; Nam, Jong-Hoon; Peterson, E. H.; Grant, Wally

doi:10.1016/j.heares.2004.06.006

Cited by 38 publications

(48 citation statements)

References 25 publications

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“…Understanding the mechanisms and regulation of this plasticity and its relationship to the functioning of mechanosensitivity is work for the future, but certainly present knowledge suggests the sensory hair bundle is much more complex than simple passive descriptions might indicate. In addition, recent modeling data indicate that the bundle may not simply rotate about its base but that there may be movement between stereocilia serving to recruit rows of stereocilia as the mechanical displacement is increased Silber et al, 2004). Despite the relatively simple description of the passive hair bundle presented below, the gating-spring theory adequately reproduces most published data.…”

Section: Hair Bundle Structurementioning

confidence: 70%

“…Size and shape of hair bundles are presumed to determine passive hair bundle properties such that tall bundles are more compliant than shorter hair bundles ( Fig. 1) Silber et al, 2004). Side links are thought to bind stereocilia together and to be important in determining the overall hair bundle maturation and stiffness; however, there is limited evidence on the relative importance of the side links (Basthtanov et al 2004;Michel et al, 2005).…”

Section: Hair Bundle Structurementioning

confidence: 99%

“…The possible different locations of the channels would contribute to inhomogeneities of channel engagement. Also the assumptions that the channels are in parallel is likely an oversimplification based on the structure of the hair bundle Silber et al, 2004. Although the hair bundle is considered to rotate only around its base, it is possible that there are micromechanical movements that serve to recruit rows of stereocilia as stimulus is increased, suggesting the channels are in fact in some combination of series and parallel.…”

Section: Gating Compliancementioning

confidence: 99%

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Mechano-electrical Transduction: New Insights into Old Ideas

et al. 2006

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The gating-spring theory of hair cell mechanotransduction channel activation was first postulated over twenty years ago. The basic tenets of this hypothesis have been reaffirmed in hair cells from both auditory and vestibular systems and across species. In fact, the basic findings have been reproduced in every hair cell type tested. A great deal of information regarding the structural, mechanical, molecular and biophysical properties of the sensory hair bundle and the mechanotransducer channel has accumulated over the past twenty years. The goal of this review is to investigate new data, using the gating spring hypothesis as the framework for discussion. Mechanisms of channel gating are presented in reference to the need for a molecular gating spring or for tethering to the intra-or extracellular compartments. Dynamics of the sensory hair bundle and the presence of motor proteins are discussed in reference to passive contributions of the hair bundle to gating compliance. And finally, the molecular identity of the channel is discussed in reference to known intrinsic properties of the native transducer channel.

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Section: Hair Bundle Structurementioning

confidence: 70%

Section: Hair Bundle Structurementioning

confidence: 99%

Section: Gating Compliancementioning

confidence: 99%

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Mechano-electrical Transduction: New Insights into Old Ideas

et al. 2006

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“…transmitted light microscopy), and the depiction of convoluted structures and the basal regions of cells. Electron microscopy (EM) has also been used to create solid models from thin serial sections (Silber et al, 2004). However EM also requires fixed samples that preclude its use in correlating computational analysis and experimentation on the same cells.…”

Section: Discussionmentioning

confidence: 99%

High-resolution solid modeling of biological samples imaged with 3D fluorescence microscopy

Ferko

Patterson

Butler

2006

Microsc. Res. Tech.

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Optical-sectioning, digital fluorescence microscopy provides images representing temporally-and spatially-resolved molecular-scale details of the substructures of living cells. To render such images into solid models for further computational analyses, we have developed an integrated system of image acquisition, processing, and rendering, which includes a new empirical technique to correct for axial distortions inherent in fluorescence microscopy due to refractive index mismatches between microscope objective immersion medium, coverslip glass, and water. This system takes advantage of the capabilities of ultra-high numerical aperture objectives (e.g. total internal reflection fluorescence microscopy) and enables faithful three-dimensional rendering of living cells into solid models amenable to further computational analysis. An example of solid modeling of bovine aortic endothelial cells and their nuclei is presented. Since many cellular level events are temporally and spatially confined, such integrated image acquisition, processing, rendering, and computational analysis, will enable, in silico, the generation of new computational models for cell mechanics and signaling.

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“…Nevertheless, mathematical modeling suggests that variation in the quantity of lateral links within the bundle is likely to impact on stiffness (Silber et al, 2004) and that upper shaft connectors might contribute most to bundle stiffness . This might explain observations that loss of top connectors substantially affects cochlear non-linearity (Verpy et al, 2011).…”

Section: Lateral Linksmentioning

confidence: 99%

The composition and role of cross links in mechanoelectrical transduction in vertebrate sensory hair cells

Hackney

Furness

2013

Journal of Cell Science

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SummaryThe key components of acousticolateralis systems (lateral line, hearing and balance) are sensory hair cells. At their apex, these cells have a bundle of specialized cellular protrusions, which are modified actin-containing microvilli, connected together by extracellular filaments called cross links. Stereociliary deflections open nonselective cation channels allowing ions from the extracellular environment into the cell, a process called mechanoelectrical transduction. This produces a receptor potential that causes the release of the excitatory neurotransmitter glutamate onto the terminals of the sensory nerve fibres, which connect to the cell base, causing nerve signals to be sent to the brain. Identification of the cellular mechanisms underlying mechanoelectrical transduction and of some of the proteins involved has been assisted by research into the genetics of deafness, molecular biology and mechanical measurements of function. It is thought that one type of cross link, the tip link, is composed of cadherin 23 and protocadherin 15, and gates the transduction channel when the bundle is deflected. Another type of link, called lateral (or horizontal) links, maintains optimal bundle cohesion and stiffness for transduction. This Commentary summarizes the information currently available about the structure, function and composition of the links and how they might be relevant to human hearing impairment.

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Computational models of hair cell bundle mechanics: III. 3-D utricular bundles

Cited by 38 publications

References 25 publications

Mechano-electrical Transduction: New Insights into Old Ideas

Mechano-electrical Transduction: New Insights into Old Ideas

High-resolution solid modeling of biological samples imaged with 3D fluorescence microscopy

The composition and role of cross links in mechanoelectrical transduction in vertebrate sensory hair cells

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