Computational identification and phylogenetic analysis of the oil-body structural proteins, oleosin and caleosin, in castor bean and flax

Hyun, Tae Kyung; Kumar, Dhinesh; Cho, Young-Yeol; Hyun, Hae-Nam; Kim, Ju-Sung

doi:10.1016/j.gene.2012.11.065

Cited by 31 publications

(18 citation statements)

References 46 publications

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“…(1) Five oleosin lineages exist, one in green algae and primitive plants, one universal in all land plants, and three in specific organs/ phylogeny of seed plants. These five lineages expand and realign from but include the two to three oleosin lineages described earlier Umate, 2012;Hyun et al, 2013;Fang et al, 2014). (2) The number of oleosin genes in each of the five lineages usually increased immediately after their evolutionary appearance and phylogeny advancement and then has remained one to four per haploid genome.…”

Section: Resultsmentioning

confidence: 99%

“…Several bioinformatics studies of oleosins have been reported recently. They involved the analysis of limited numbers of oleosin genes (73,21,17, and approximately 100 by Liu et al [2012], Umate [2012], Hyun et al [2013], and Fang et al [2014], respectively) in restricted species and did not consider sufficiently the biological relevance and drew some unsubstantiated conclusions. These conclusions included that, during evolution, species-specific expansion in oleosin gene numbers continued to expand from primitive plants to seed plants, that pollen-specific oleosins had been subjected to a high degree of evolutionary dynamics, and that the introns were gained early on evolution and then lost.…”

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confidence: 99%

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Bioinformatics Reveal Five Lineages of Oleosins and the Mechanism of Lineage Evolution Related to Structure/Function from Green Algae to Seed Plants

Huang

2015

Plant Physiol.

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Plant cells contain subcellular lipid droplets with a triacylglycerol matrix enclosed by a layer of phospholipids and the small structural protein oleosin. Oleosins possess a conserved central hydrophobic hairpin of approximately 72 residues penetrating into the lipid droplet matrix and amphipathic amino-and carboxyl (C)-terminal peptides lying on the phospholipid surface. Bioinformatics of 1,000 oleosins of green algae and all plants emphasizing biological implications reveal five oleosin lineages: primitive (in green algae, mosses, and ferns), universal (U; all land plants), and three in specific organs or phylogenetic groups, termed seed low-molecular-weight (SL; seed plants), seed high-molecular-weight (SH; angiosperms), and tapetum (T; Brassicaceae) oleosins. Transition from one lineage to the next is depicted from lineage intermediates at junctions of phylogeny and organ distributions. Within a species, each lineage, except the T oleosin lineage, has one to four genes per haploid genome, only approximately two of which are active. Primitive oleosins already possess all the general characteristics of oleosins. U oleosins have C-terminal sequences as highly conserved as the hairpin sequences; thus, U oleosins including their C-terminal peptide exert indispensable, unknown functions. SL and SH oleosin transcripts in seeds are in an approximately 1:1 ratio, which suggests the occurrence of SL-SH oleosin dimers/multimers. T oleosins in Brassicaceae are encoded by rapidly evolved multitandem genes for alkane storage and transfer. Overall, oleosins have evolved to retain conserved hairpin structures but diversified for unique structures and functions in specific cells and plant families. Also, our studies reveal oleosin in avocado (Persea americana) mesocarp and no acyltransferase/lipase motifs in most oleosins.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Bioinformatics Reveal Five Lineages of Oleosins and the Mechanism of Lineage Evolution Related to Structure/Function from Green Algae to Seed Plants

Huang

2015

Plant Physiol.

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“…Recently, a structural protein, Oleosin3 (OLE3), was shown to exhibit MGAT activity (Parthibane et al 2012), indicating that this enzyme may play a role in the synthetic pathways of TAG, and another pathway may also exist in developing oil seed tissues, which need to be further verified. There was also a high level of oleosin expression in the Unigenes, which is the main structure-stabilizing protein of the plant oil bodies (Parthibane et al 2012;Hyun et al 2013) in the group of proteins related to oil body formation. In our study, 11 genes related to oleosin were found.…”

Section: Detection Of Sequences Related To Seed Oil Formationmentioning

confidence: 99%

New features of triacylglycerol biosynthetic pathways of peanut seeds in early developmental stages

Liu

Zhu

et al. 2015

Funct Integr Genomics

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The peanut (Arachis hypogaea L.) is one of the three most important oil crops in the world due to its high average oil content (50 %). To reveal the biosynthetic pathways of seed oil in the early developmental stages of peanut pods with the goal of improving the oil quality, we presented a method combining deep sequencing analysis of the peanut pod transcriptome and quantitative real-time PCR (RT-PCR) verification of seed oil-related genes. From the sequencing data, approximately 1500 lipid metabolism-associated Unigenes were identified. The RT-PCR results quantified the different expression patterns of these triacylglycerol (TAG) synthesis-related genes in the early developmental stages of peanut pods. Based on these results and analysis, we proposed a novel construct of the metabolic pathways involved in the biosynthesis of TAG, including the Kennedy pathway, acyl-CoA-independent pathway and proposed monoacylglycerol pathway. It showed that the biosynthetic pathways of TAG in the early developmental stages of peanut pods were much more complicated than a simple, unidirectional, linear pathway.

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“…We identified the presence of oleosins and caleosins proteins in camellia seed, and involved in 4 oleosin unigenes (CL734Contig1, CL422Contig1, CL32127Contig1 and CL25464Contig1) with expression over 1000 RPKM in average (Table 7, S4). So oleosin is the most abundant structural protein in camellia oil bodies like other oilseed crops (Hyun et al 2013;Yu et al 2015;Parthibane et al 2012).…”

Section: Discussion Fatty Acids Biosynthesis and Accumulationmentioning

confidence: 99%

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

Feng

Yang

Weiwei

et al. 2017

Trees

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Key message The molecular mechanisms of fatty acid biosynthesis and accumulation during Camellia meiocarpa and Camellia oleifera seed natural drying are characterized through transcriptome analyses. Abstract Camellia seed oil has been used as high quality and healthy food for over two thousand years. Seed drying affects oil quality and quantity; however, the molecular mechanisms of fatty acid biosynthesis and accumulation during the drying process remain unknown. In this study, the transcriptomes of Camellia meiocarpa and C. oleifera seed were characterized at five moisture content levels (10-50%) to identify the major processes and reveal genes affecting lipid metabolism in response to natural drying. We found a total of 111,156 unigenes by de novo assembled from RNA-Seq libraries of five moisture content levels during after-ripening of C. meiocarpa (74,016) and C. oleifera (76,374). Ten pathways were closely linked to changes in oil content and composition with 244 genes involved in fatty acid synthesis and accumulation. Gene ontology enrichment of differentially expressed genes (DEGs) indicated that fatty acid synthesis and accumulation are essential in C. meiocarpa while fatty acid accumulation in C. oleifera during natural drying process. Comparative analyses of DEGs between any two consecutive moisture contents identified six and three key unigenes in C. meiocarpa and C. oleifera seeds, respectively, and one additional unigene responsible for the difference between the two species' fatty acid synthesis and accumulation. Natural drying has improved the quality and quantity of the camellia seed oil. The study provided: (a) global transcriptional profiles at five moisture content levels during seed natural drying, (b) highlighting transcripts putatively involved in the regulation of gene expression program and in specific processes likely essential for lipid metabolism, and (c) discovery of genes associated with oil seed quantity and quality improvement for the studied two camellia species.Communicated by M. Buckeridge. Electronic supplementary materialThe online version of this article

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Computational identification and phylogenetic analysis of the oil-body structural proteins, oleosin and caleosin, in castor bean and flax

Cited by 31 publications

References 46 publications

Bioinformatics Reveal Five Lineages of Oleosins and the Mechanism of Lineage Evolution Related to Structure/Function from Green Algae to Seed Plants

Bioinformatics Reveal Five Lineages of Oleosins and the Mechanism of Lineage Evolution Related to Structure/Function from Green Algae to Seed Plants

New features of triacylglycerol biosynthetic pathways of peanut seeds in early developmental stages

Transcriptome comparative analysis of two Camellia species reveals lipid metabolism during mature seed natural drying

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