Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

Cinbiz, Mahmut Nedim; Tiğli, Ragıp; Beşkardeş, Işıl Gerçek; Gümüşderelı́oğlu, Menemşe; Çolak, Üner

doi:10.1016/j.jbiotec.2010.09.950

Cited by 28 publications

(12 citation statements)

References 26 publications

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“…Active methods for improving nutrient uptake include bioreactors that apply compression 24,29,30,35,36,75,80 or rotation 102,103 and perfusion devices. 78,80,104,105 As discussed in the previous section, physiological levels of compressive dynamic loading ($10% strain, less than 6 hours) may be ideal for enhancing nutrient diffusion into engineered cartilage.…”

Section: Resultsmentioning

confidence: 99%

Toward Engineering a Biological Joint Replacement

et al. 2012

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Osteoarthritis is a major cause of disability and pain for patients in the United States. Treatments for this degenerative disease represent a significant challenge considering the poor regenerative capacity of adult articular cartilage. Tissue-engineering techniques have advanced over the last two decades such that cartilage-like tissue can be cultivated in the laboratory for implantation. Even so, major challenges remain for creating fully functional tissue. This review article overviews some of these challenges, including overcoming limitations in nutrient supply to cartilage, improving in vitro collagen production, improving integration of engineered cartilage with native tissue, and exploring the potential for engineering full articular surface replacements. Keywordsbiological joint replacement; cartilage; tissue-engineering; nutrient diffusion; collagen production Over 20% of the adults in the United States (25 years and above) have osteoarthritis (OA) of the hip or knee (>46 million Americans). 1 OA is ranked as one of the top three causes for disability and contributes more than $185 billion dollars a year in related medical costs. [2][3][4] More than 580,000 arthroplasty procedures are performed each year in the U.S. 5 While joint replacement generally succeeds in decreasing or eliminating pain and restoring joint function, the lifespan of prostheses are limited due to wear, loosening, infection, and fracture of the implant or surrounding bone. 6-9 Alternative treatments have not yet been successful in providing a viable long-term option for cartilage repair. For example, allografts are limited by donor tissue availability and graft viability, 10,11 while autografts are limited by the availability of healthy tissue and donor site morbidity. Bio-engineered repair strategies that circumvent these limitations, while preserving the natural function of the joint and using Copyright © 2012 NIH-PA Author ManuscriptNIH-PA Author Manuscript NIH-PA Author Manuscript a procedure less invasive than total joint arthroplasty, may be optimal for treating younger OA patients.Articular cartilage serves as the load-bearing material of joints and possesses excellent friction, lubrication, and wear characteristics. 12 Successful replacement of damaged or injured articular cartilage will hinge on the ability to recapitulate the mechanical and structural properties of the healthy native tissue before implantation. Over the past two decades, there has been a wide interest in developing functional engineered cartilage. To grow cartilage tissue, cells are cultured within a three-dimensional (3D) scaffold that provides an initial structure for the de novo tissue [13][14][15] ; alternatively, cells may be cultured using scaffold-less techniques. 16,17 For example, autologous chondrocyte implantation (ACI) is a cell-based strategy where cells are injected directly into focal lesions and covered with a periosteal flap 18,19 whereas CARTIPATCH 18 uses a 3D agarose hydrogel scaffold to prevent leakage of cells, stabilize t...

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Section: Resultsmentioning

confidence: 99%

Toward Engineering a Biological Joint Replacement

et al. 2012

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“…42 This motion of the samples relative to the fluid generates fluid shear forces on a particle surface ranging from 180-320 mPa (0.18-0.32 dyne/cm 2 ) for 50-mm beads 43 and *500 mPa (0.5 dyne/cm 2 ) with 3D aggregates of BHK-21 cells 44 to 520-780 mPa (5.2-7.8 dynes/cm 2 ) for a 200-or 300-mm spherical object. 45 Over the years various models based on the initial RWV have been developed, differing in vessel geometry and aspect ratio and gas supply (perfusion), such as the 250-mL, slow-turning lateral vessel (Synthecon) 46 or the 50-mL high aspect ratio vessel (HARV; Synthecon) 47 or the rotating-wall perfused vessel (Synthecon) 48 ( Fig. 1D, E).…”

Section: Random-positioning Machinementioning

confidence: 99%

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Grimm

Wehland

Pietsch

et al. 2014

Tissue Engineering Part B: Reviews

121

137

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Tissue engineering in simulated (s-) and real microgravity (r-mg) is currently a topic in Space medicine contributing to biomedical sciences and their applications on Earth. The principal aim of this review is to highlight the advances and accomplishments in the field of tissue engineering that could be achieved by culturing cells in Space or by devices created to simulate microgravity on Earth. Understanding the biology of three-dimensional (3D) multicellular structures is very important for a more complete appreciation of in vivo tissue function and advancing in vitro tissue engineering efforts. Various cells exposed to r-mg in Space or to s-mg created by a random positioning machine, a 2D-clinostat, or a rotating wall vessel bioreactor grew in the form of 3D tissues. Hence, these methods represent a new strategy for tissue engineering of a variety of tissues, such as regenerated cartilage, artificial vessel constructs, and other organ tissues as well as multicellular cancer spheroids. These aggregates are used to study molecular mechanisms involved in angiogenesis, cancer development, and biology and for pharmacological testing of, for example, chemotherapeutic drugs or inhibitors of neoangiogenesis. Moreover, they are useful for studying multicellular responses in toxicology and radiation biology, or for performing coculture experiments. The future will show whether these tissue-engineered constructs can be used for medical transplantations. Unveiling the mechanisms of microgravity-dependent molecular and cellular changes is an up-to-date requirement for improving Space medicine and developing new treatment strategies that can be translated to in vivo models while reducing the use of laboratory animals.

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“…Также разрабо-тана математическая модель для культивирования хондроцитов, заселенных в пористый скаффолд в условиях перфузии и с двусторонним направлени-ем потока [135]. Отметим, что некоторые группы ученых вначале создавали компьютерную модель биореактора, а лишь потом применили ее на прак-тике [118,136].…”

Section: заключениеunclassified

Tissue Engineering and Regenerative Medicine Technologies in the Treatment of Articular Cartilage Defects

Басок

Севастьянов

2017

RJTAO

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1 ФГБУ «Федеральный научный центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России, Москва, Российская Федерация 2 АНО «Институт медико-биологических исследований и технологий», Москва, Российская Федерация Важнейшими проблемами здравоохранения в индустриальном обществе являются повреждение и деге-нерация суставного хряща, что связано с ограниченными возможностями ткани к регенерации. В обзоре подробно описаны существующие и разрабатываемые технологии восстановления и замещения повреж-денных хрящевых тканей суставов. Дан анализ полученных результатов по двум основным направлени-ям: стимулирование регенерации поврежденной хрящевой ткани и выращивание элементов хрящевой ткани в биореакторах.Ключевые слова: суставной хрящ, клеточно-и тканеинженерные конструкции, регенеративная медицина, биореакторы. TISSUE ENGINEERING AND REGENERATIVE MEDICINE TECHNOLOGIES IN THE TREATMENT OF ARTICULAR CARTILAGE DEFECTS

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Computational fluid dynamics modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering

Cited by 28 publications

References 26 publications

Toward Engineering a Biological Joint Replacement

Toward Engineering a Biological Joint Replacement

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Tissue Engineering and Regenerative Medicine Technologies in the Treatment of Articular Cartilage Defects

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