Computational Fluid Dynamics Investigation of the Effect of the Fluid-Induced Shear Stress on Hepatocyte Sandwich Perfusion Culture

Leo, Hwa Liang; Lei, Xia; Ng, Shi Ling; Poh, Hee Joo; Zhang, S. F.; Cheng, Tina; Xiao, Guangfa; Tuo, Xiaoye; Yu, Hanry

doi:10.1007/978-3-540-92841-6_347

Cited by 3 publications

(2 citation statements)

References 3 publications

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“…Complex geometries, in particular the presence of sharp angles, can lead to irregularities in the fluid flow and therefore mass transfer and shear stress [200] , [201] , [202] , as well as leading to an increase in turbulence. Tissue culture bioreactors frequently utilise laminar flow, in which the motion of fluid particles is parallel to the bulk flow of the fluid, to create the desired conditions due to its uniformity and ease of modelling.…”

Section: Fluid Flow Parametersmentioning

confidence: 99%

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Hoyle

Stenger

2022

Biomaterials and Biosystems

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Section: Fluid Flow Parametersmentioning

confidence: 99%

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Hoyle

Stenger

2022

Biomaterials and Biosystems

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“…For example, for experiments on liver cells, Alvetex Strata was used and stirring performed at 80 r.p.m. This creates levels of shear stress below 10 −3 dyne cm −2 , which have been shown to have positive effects on hepatocyte metabolism [23,24] as well as being significantly below levels which reduce hepatocyte viability [25].…”

Section: Bench-top Perfusion Systemmentioning

confidence: 99%

Applications of novel bioreactor technology to enhance the viability and function of cultured cells and tissues

2020

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As the field of tissue engineering continues to advance rapidly, so too does the complexity of cell culture techniques used to generate in vitro tissue constructs, with the overall aim of mimicking the in vivo microenvironment. This complexity typically comes at a cost with regards to the size of the equipment required and associated expenses. We have developed a small, low-cost bioreactor system which overcomes some of the issues of typical bioreactor systems while retaining a suitable scale for the formation of complex tissues. Herein, we have tested this system with three cell populations/tissues: the culture of hepatocellular carcinoma cells, where an improved structure and basic metabolic function is seen; the culture of human pluripotent stem cells, in which the cultures can form more heterogeneous tissues resembling the in vivo teratoma and ex vivo liver tissue slices, in which improved maintenance of cellular viability is seen over the 3 days tested. This system has the flexibility to be used for a variety of further uses and has the potential to provide a more accessible alternative to current bioreactor technologies.

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The Application of Computational Simulation Technology in Bioartificial Liver System

Huo¹,

Cao²,

Wu³

et al. 2010

2010 4th International Conference on Bioinformatics and Biomedical Engineering

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Computational Fluid Dynamics Investigation of the Effect of the Fluid-Induced Shear Stress on Hepatocyte Sandwich Perfusion Culture

Cited by 3 publications

References 3 publications

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

Applications of novel bioreactor technology to enhance the viability and function of cultured cells and tissues

The Application of Computational Simulation Technology in Bioartificial Liver System

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