Computational fluid dynamics for improved bioreactor design and 3D culture

Hutmacher, Dietmar W.; Singh, Harmeet

doi:10.1016/j.tibtech.2007.11.012

Cited by 184 publications

(99 citation statements)

References 28 publications

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“…The integration of mathematical tools in the design is therefore of particular interest in order to facilitate the work, for example using statistical approaches, design-of-experiments [40] and mechanistic modelling [41,42].…”

Section: Discussionmentioning

confidence: 99%

Scale‐up of cell culture bioreactors using biomechatronic design

Mandenius

Björkman

2012

Biotechnology Journal

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An alternative approach for scale-up of cell culture bioreactors based on biomechatronic design methodology is suggested.The approach differs from traditional biochemical engineering methodology by applying a sequential design procedure where the needs of the users and alternative design solutions based on the biological and technical functions of the scaled-up bioreactor are derived in functional maps, concept generation charts and scoring and interaction matrices. Basic reactor engineering properties, such mass and heat transfer and kinetics are integrated in the procedure. Examples are provided from production of monoclonal antibody and recombinant protein.

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Section: Discussionmentioning

confidence: 99%

Scale‐up of cell culture bioreactors using biomechatronic design

Mandenius

Björkman

2012

Biotechnology Journal

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“…However, despite all of these technological efforts, none of these bioreactors, are, at present, able to provide optimal conditions for the long-term maintenance of large tissue-like masses in culture. The current generation of bioreactors was, in reality, developed for yielding large masses of cells (or cell products) for industrial or clinical applications, and not for supporting the survival or the self-assembly of multiple cell types into complex 3D tissue-like structures (Hutmacher & Singh, 2008). This applies, in particular, to the liver, a highly specialized organ, whose cellular components are extremely sensitive to even minimal environmental changes, already under physiological conditions (Nahmias et al, 2006;Catapano & Gerlach, 2007).…”

Section: Bioreactors and Relative Microgravity Conditionmentioning

confidence: 99%

New Models for the In Vitro Study of Liver Toxicity: 3D Culture Systems and the Role of Bioreactors

Mazzoleni¹,

Steimberg²

2012

The Continuum of Health Risk Assessments

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“…The current generation of bioreactors has, in fact, been developed to support the rapid growth of cells in solution and not the culturing of tissue engineered constructs [38].…”

Section: Bioreactorsmentioning

confidence: 99%

“…Computational fluid modelling will be a powerful tool as well, that, by contributing to generating and optimising tissue engineering-related bioreactors, can be helpful in reproducing specific physiological environments for 3-D cell constructs [38]. Model systems made of different compartments configured for representing the various individual tissues/organs (functional tissue equivalents) could be then generated and hierarchically connected by controlled medium exchanges, designed to reproduce the metabolic interactions that physiologically take place between organs, thus simulating the situation of a whole living organism.…”

Section: Tissue Engineering and Microtechnologies: The Perspectivesmentioning

confidence: 99%

Modelling tissues in 3D: the next future of pharmaco-toxicology and food research?

2008

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The development and validation of reliable in vitro methods alternative to conventional in vivo studies in experimental animals is a well-recognised priority in the fields of pharmaco-toxicology and food research. Conventional studies based on two-dimensional (2-D) cell monolayers have demonstrated their significant limitations: the chemically and spatially defined three-dimensional (3-D) network of extracellular matrix components, cell-to-cell and cell-to-matrix interactions that governs differentiation, proliferation and function of cells in vivo is, in fact, lost under the simplified 2-D condition. Being able to reproduce specific tissue-like structures and to mimic functions and responses of real tissues in a way that is more physiologically relevant than what can be achieved through traditional 2-D cell monolayers, 3-D cell culture represents a potential bridge to cover the gap between animal models and human studies. This article addresses the significance and the potential of 3-D in vitro systems to improve the predictive value of cell-based assays for safety and risk assessment studies and for new drugs development and testing. The crucial role of tissue engineering and of the new microscale technologies for improving and optimising these models, as well as the necessity of developing new protocols and analytical methods for their full exploitation, will be also discussed.

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Computational fluid dynamics for improved bioreactor design and 3D culture

Cited by 184 publications

References 28 publications

Scale‐up of cell culture bioreactors using biomechatronic design

Scale‐up of cell culture bioreactors using biomechatronic design

New Models for the In Vitro Study of Liver Toxicity: 3D Culture Systems and the Role of Bioreactors

Modelling tissues in 3D: the next future of pharmaco-toxicology and food research?

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