“…The function of a binding pocket within a protein is not only determined by its structure and location but also influenced by the specific group of amino acid residues that surround it [28]. The binding pocket residues of GABA-AT were chosen from already published data [27], in which we calculated the binding pocket residues of human GABA-AT using an online webserver, PrankWeb (https://prankweb.cz/) (accessed on 25 March 2023), and selected as Ile100, Ser102, Ala162, Cys163, Gly164, Ser165, Phe217, His218, Gly219, Arg220, Glu293, Asp326, Val328, Gln_329, Gln330, Ser356, Lys357, and Met360. Moreover, the binding pocket of GABA-AT was visualized using Discovery Studio and UCSF Chimera to confirm the residual binding position (Figure 2).…”