Computational de-scattering for enabling high rate deep imaging of neural activity traces: Simulation study

Paluch, Shir; Dana, Hod; Elbaz, Romi; Shoham, Sharon

doi:10.1109/ner.2013.6696239

Cited by 1 publication

(1 citation statement)

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“…Despite the growing popularity of SPIM, the fundamental imaging depth limit of this microscopy technique has yet to characterized either theoretically or experimentally. Paluch et al [19] reported that in their experiments the signal strength and the noise strength are equal at 10 MFPs, but did not characterize the factors that affect this maximum imaging depth. Here, Fig.…”

Section: Spimmentioning

confidence: 92%

Deep imaging in scattering media with selective plane illumination microscopy

et al. 2016

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In most biological tissues, light scattering due to small differences in refractive index limits the depth of optical imaging systems. Two-photon microscopy (2PM), which significantly reduces the scattering of the excitation light, has emerged as the most common method to image deep within scattering biological tissue. This technique, however, requires high-power pulsed lasers that are both expensive and difficult to integrate into compact portable systems. Using a combination of theoretical and experimental techniques, we show that if the excitation path length can be minimized, selective plane illumination microscopy (SPIM) can image nearly as deep as 2PM without the need for a high-powered pulsed laser. Compared to other single-photon imaging techniques like epifluorescence and confocal microscopy, SPIM can image more than twice as deep in scattering media ( ? 10 times the mean scattering length). These results suggest that SPIM has the potential to provide deep imaging in scattering media in situations in which 2PM systems would be too large or costly.

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Section: Spimmentioning

confidence: 92%