Abstract:Systemic Lupus Erythematosus is a debilitating autoimmune disease that afflicts about 1.5 million Americans today. Although SLE is a multifactorial disease, SLE can arise with disruptions in two genes: Dnase1 and Dnase1L3. While the structure is known for DNase1, the structure for Dnase1L3 is unknown. To study the structure and function of the Dnase1L3 enzyme, we designed a heterologous expression system and purification protocol for mouse DNase1L3 in E. coli. This system will allow us to characterize the enzy… Show more
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