Computational and experimental comparison on the effects of flow-induced compression on the permeability of collagen gels

Vidmar, Christopher S.; Bazzi, Marisa S.; Lai, Victor K.

doi:10.1016/j.jmbbm.2022.105107

Cited by 3 publications

(3 citation statements)

References 59 publications

(31 reference statements)

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“…We previously measured by tensile tests the Young modulus of 6 and 10 mg/mL collagen I gels employed in our study at 55±15 kPa to 86±47 kPa respectively (Bernheim-Dennery et al, unpublished data). The relative increase in Young modulus thus remains moderate and is corroborated by a comprehensive review of existing literature, where we observe a quadratic relationship between the gel's Young's modulus and its concentration [50][51][52][53] . This matrix modification limited tubular dilation under flow + pressure conditions for parental cells of both cell types, but more markedly for mIMCD-3 cells.…”

Section: Hydrodynamic Constraints Differentially Affect the Ability Ofsupporting

confidence: 83%

Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip

Lapin,

Gropplero,

Vandensteen

et al. 2024

Preprint

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Kidney tubular cells are submitted to two distinct mechanical forces generated by the urine flow: shear stress and hydrostatic pressure. In addition, the mechanical properties of the surrounding extracellular matrix modulate tubule deformation under constraints. These mechanical factors likely play a role in the pathophysiology of kidney diseases as exemplified by autosomal dominant polycystic kidney disease, in which pressure, flow and matrix stiffness have been proposed to modulate the cystic dilation of tubules withPKD1mutations. The lack ofin vitrosystems recapitulating the mechanical environment of kidney tubules impedes our ability to dissect the role of these mechanical factors. Here we describe a perfused kidney-on-chip with tunable extracellular matrix mechanical properties and hydrodynamic constraints, that allows a decoupling of shear stress and flow. We used this system to dissect how these mechanical cues affectPkd1-/-tubule dilation. Our results show two distinct mechanisms leading to tubular dilation. For PCT cells (proximal tubule), overproliferation mechanically leads to tubular dilation, regardless of the mechanical context. For mIMCD-3 cells (collecting duct), tube dilation is associated with a squamous cell morphology but not with overproliferation and is highly sensitive to extracellular matrix properties and hydrodynamic constraints. Surprisingly, flow alone suppressedPkd1-/-mIMCD-3 tubule dilation observed in static conditions, while the addition of luminal pressure restored it. Ourin vitromodel emulating nephron geometrical and mechanical organization sheds light on the roles of mechanical constraints in ADPKD and demonstrates the importance of controlling intraluminal pressure in kidney tubule models.

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Section: Hydrodynamic Constraints Differentially Affect the Ability Ofsupporting

confidence: 83%

Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip

Lapin,

Gropplero,

Vandensteen

et al. 2024

Preprint

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“…35,36 Under load, polymeric membranes experience deformation that modifies the microstructure. Consistent with other polymeric membrane systems, 27,37,38 PDMS's permeability to water vapor has been found to decrease as the chain mobility decreases; 29 mobility was controlled by changing crosslink density and verified with its proportionality to the glass transition temperature. Notably, Song, et al 39 reported stretch dependent permeability of ethanol through PDMS.…”

Section: Introductionsupporting

confidence: 78%

Deformation-dependent polydimethylsiloxane permeability measured using osmotic microactuators

Spitzer,

Hutchens

2023

Soft Matter

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“…Our method is based on a pressure sensor connected to an air cavity placed at the outlet of the material and a pressure controller to monitor the inlet. Therefore, this method relies on two sensors, whereas conventional techniques use pressure, flow, and deformation detection units (21). Supported by an analytical model to extract the permeability and elasticity of the gel and validated by finite element simulations, our instrument uncovers the asymmetry of the permeation and elastic properties of collagen gels for tensile and compressive stresses in the regime of reversible deformation, i.e., without permanent reorganization of the gel network as observed when applying the load with a mechanical piston (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Asymmetry of tensile versus compressive elasticity and permeability contributes to the regulation of exchanges in collagen gels

et al. 2023

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The Starling principle describes exchanges between blood and tissues based on the balance of hydrostatic and osmotic flows. However, the permeation properties of the main constituent of tissues, namely, collagen, in response to the stress exerted by blood pressure remain poorly characterized. Here, we develop an instrument to determine the elasticity and permeability of collagen gels under tensile and compressive stress based on measuring the temporal change in pressure in an air cavity sealed at the outlet of a collagen slab. Data analysis with an analytical model reveals a drop in the permeability and enhanced strain stiffening of native collagen gels under compression versus tension, both effects being essentially lost after chemical cross-linking. Furthermore, we report the control of the permeability of native collagen gels using sinusoidal fluid injection, an effect explained by the asymmetric response in tension and compression. We lastly suggest that blood-associated pulsations could contribute to exchanges within tissues.

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Computational and experimental comparison on the effects of flow-induced compression on the permeability of collagen gels

Cited by 3 publications

References 59 publications

Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip

Decoupling shear stress and pressure effects in the biomechanics of autosomal dominant polycystic kidney disease using a perfused kidney-on-chip

Deformation-dependent polydimethylsiloxane permeability measured using osmotic microactuators

Asymmetry of tensile versus compressive elasticity and permeability contributes to the regulation of exchanges in collagen gels

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