Computational Analysis of Single Nucleotide polymorphisms (SNPs) in Human TCell Acute Lymphocytic Leukemia Protein 1 (TAL1) Gene/Comprehensive Study

Abstract

Background: TAL1 is a proto-oncogene whose distorted modifications in committed T-cell Precursors is related with the development of T-ALL, it also found to be related to many other human hematological diseases such as lymphoblastic lymphoma, immunodeficiency 18, acute myeloid leukemia and diamond-blackfan Anemia. Objectives: This study aims to predict the effect of nsSNPs on TAL1 protein structure function Methods: Retrieved nSNPs in the coding and 3'UTR regions were analyzed using different in silico tools. Interactions of TAL1 with functionally similar genes were investigated using Genemania. Post-translational modifications in several sites of the protein were also investigated. Results: Out of ninety nsSNPs identified, only eight were found damaging to protein function of which one is located in the basis helix-loop-helix domain (bHLH). Two SNPs were anticipated by PolymiRTs to prompt disturbance or creation of miR binding sites. Conclusion: The present study is the first ever computational analysis of TAL1's nsSNPs hence this effort might be of help in the near future for inventing early diagnostic and therapeutic measures for T-ALL * Function Class: D: The derived allele disrupts a conserved miRNA site (ancestral allele with support ≥2). N: The derived allele disrupts a nonconserved miRNA site (ancestral allele with support < 2). C: The derived allele creates a new miRNA site. O: The ancestral allele cannot be determined.

Mutational analysis on predicting the impact of high-risk SNPs in human secretary phospholipase A2 receptor (PLA2R1)

“…Numerous studies in the past have conducted various analysis particularly in-silico analysis that combines bioinformatics tool to predict the structural and functional consequences of nsSNPs occurring at the coding region of the genome. One study conducted SNP analysis in TAL1 gene which is a proto-oncogene hence is found associated with various hematological diseases 13 . Another study performed computational analysis on glycoprotein M6A that identified nsSNPs which are damaging to the structure and function of the protein 14 .…”