Computational 3D model of in-vitro cell stimulated by electric and magnetic fields

Abstract

This work presents the development of 3D computational models that represent two studies about in-vitro cellular experimentation of cell stimulated by magnetic and electric field. The development considered the construction of the stimulation devices, the cell seeding, and the creation of the 3D computational models representing the arrangements. The models and their electromagnetic analysis were done in the ANSYS program. The volumes considered were: source of stimulation, Falcon cell culture plate, cell content, and space for zero potential. The electric field stimulation model considered an applied electric field between 250 V/m and 1 kV/m. While the magnetic field stimulation model considered an applied magnetic field between 0.5 mT and 2.0 mT. For both models, the frequency range was between 5 Hz and 105 Hz. As a result, the error between the stimulation devices and the created models was lower than 5%. The homogeneous area of the magnetic and electric field was established and the behavior of field strength produced by the stimulation devices was the expected one. In both models, the induced current density was the variable evaluated in the cellular material. The current density induced by the applied magnetic field was greater than by the applied electric field.