Comprehensive transcriptomic analysis of the process of gonadal sex differentiation in the turbot ( Scophthalmus maximus )

Ribas, Laia; Robledo, Diego; Gómez-Tato, Antonio; Viñas, Ana; Martı́nez, Paulino; Piferrer, Francesc

doi:10.1016/j.mce.2015.11.006

Cited by 51 publications

(47 citation statements)

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“…These genes were sulfotransferase family cytosolic 1B member 1 (hereafter SULT1B1 ) and pre‐mRNA‐splicing factor cwf19 (hereafter cwf19) and are involved in steroid metabolism and mRNA splicing, respectively. Both genes that were previously reported to influence sex determination in fishes (Devlin & Nagahama, ), namely in European Eel ( Anguilla anguilla; Churcher et al., ) and Turbot ( Scophthalmus maximus; Ribas et al., ).…”

Section: Resultsmentioning

confidence: 98%

“…Steroids play important roles in regulating physiological functions related to reproduction and sex differentiation in fishes (James, ). Sulfotransferase genes, such as SULT1 , are linked to sex determination in house mouse ( Mus musculus ; Dunn, Gleason, & Hartley, ), mussels ( Mytilus galloprovincialis ; Atasaral Şahin et al., ), European Eel ( Anguilla anguilla; Churcher et al., ) and Turbot ( Scophthalmus maximus; Ribas et al., ). For instance, sulfotransferase 6B1‐like gene ( SULT6B1 ) was expressed at higher levels in the livers of sexually mature European Eel males relative to females (Churcher et al., ).…”

Section: Discussionmentioning

confidence: 99%

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Sex matters in massive parallel sequencing: Evidence for biases in genetic parameter estimation and investigation of sex determination systems

et al. 2017

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Using massively parallel sequencing data from two species with different life history traits, American lobster (Homarus americanus) and Arctic Char (Salvelinus alpinus), we highlight how an unbalanced sex ratio in the samples and a few sex-linked markers may lead to false interpretations of population structure and thus to potentially erroneous management recommendations. Here, multivariate analyses revealed two genetic clusters separating samples by sex instead of by expected spatial variation: inshore and offshore locations in lobster, or east and west locations in Arctic Char. To further investigate this, we created several subsamples artificially varying the sex ratio in the inshore/offshore and east/west groups and then demonstrated that significant genetic differentiation could be observed despite panmixia in lobster, and that F values were overestimated in Arctic Char. This pattern was due to 12 and 94 sex-linked markers driving differentiation for lobster and Arctic Char, respectively. Removing sex-linked markers led to nonsignificant genetic structure in lobster and a more accurate estimation of F in Arctic Char. The locations of these markers and putative identities of genes containing or nearby the markers were determined using available transcriptomic and genomic data, and this provided new information related to sex determination in both species. Given that only 9.6% of all marine/diadromous population genomic studies to date have reported sex information, we urge researchers to collect and consider individual sex information. Sex information is therefore relevant for avoiding unexpected biases due to sex-linked markers as well as for improving our knowledge of sex determination systems in nonmodel species.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Sex matters in massive parallel sequencing: Evidence for biases in genetic parameter estimation and investigation of sex determination systems

et al. 2017

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“…Microsatellites were used both to organize broodstock according to minimum parentage (relatedness or inbreeding estimators), to allocate parents and estimate breeding values in competing mixing families (paternity analysis), and also to check the goodness of families founded in breeding programs (Castro et al, 2004). Molecular traceability tools have been later Millán et al, 2011;Díaz-Rosales et al, 2012;Pardo et al, 2012;Ribas et al, 2016). A similar pathway regarding transcriptome databases and microarray design was followed later for sea bass (Louro et al, 2010) and sea bream (Calduch-Giner et al, 2010, and for mollusks such as Manila clam (Moreira et al, 2012(Moreira et al, , 2014Romero et al, 2015) and European flat oyster .…”

Section: Main Textmentioning

confidence: 99%

Genomics advances for boosting aquaculture breeding programs in Spain

Martı́nez

2016

Aquaculture

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“…Por ello, se hace necesario aplicar técnicas que permitan tanto la identificación temprana como la identificación correcta de híbridos, como son las técnicas moleculares. Entre las alternativas podemos citar el análisis de cariotipos, la citometria de DNA (Xiao et al 2014)o el uso de téc-nicas moleculares rápidas y certeras, como los RFLPs (polimorfismos de longitud de fragmentos de restricción) o SNPs (polimorfismos de un solo nucleótido), el uso de micro-satélites, códigos de barra genéticos (DNA barcode), PCR en tiempo real y/o, buscar genes candidatos responsables de determinados fenotipos (pirosecunciación) (Hashimoto et al, 2010, Hasselman et al, 2014, Ribas et al, 2016.…”

Section: Mecanismos De Identificación De Híbridosunclassified

Hibridización de silúridos: una alternativa para la piscicultura con alto riesgo para la conservación de las especies nativas

2016

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ResumenEl propósito de este artículo es analizar, desde una mirada crítica, el riesgo a que se ven expuestas ciertas especies de silú-ridos, sometidas a procesos de hibridación por razones estrictamente comerciales, incluyendo los efectos sobre el hábitat, la competencia y la erosión genética. Es fácil comprender que dichos procedimientos tendrán en el futuro serios efectos sobre los recursos hidrobiológicos, los servicios ecosistémicos y la seguridad alimentaria, los cuales sólo pueden ser medidos a través del tiempo y no de forma inmediata.Palabras clave: Acuicultura, fitness, erosión genética, peces nativos (recursos acuáticos). AbstractThe purpose of this article was to analyze, from a critical perspective, the risk that would be exposed some silurid species in captivity, subjected to hybridization processes for strictly commercial reasons, including habitat effects, competition and genetic erosion. It is easy to understand that these procedures will be in the future effects on aquatic resources, ecosystem services and food security, which can only be measured over time and not immediately.Keywords: Aquaculture, fitness, genetic erosion, native fish (aquatic resources). ResumoO objetivo deste artigo foi analisar, a partir de uma perspectiva crítica, o risco ao qual seriam expostas algumas espécies de silúridos em cativeiro, sujeitos a processos de hibridação por razões exclusivamente comerciais, incluindo os efeitos sobre

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Comprehensive transcriptomic analysis of the process of gonadal sex differentiation in the turbot ( Scophthalmus maximus )

Cited by 51 publications

References 124 publications

Sex matters in massive parallel sequencing: Evidence for biases in genetic parameter estimation and investigation of sex determination systems

Sex matters in massive parallel sequencing: Evidence for biases in genetic parameter estimation and investigation of sex determination systems

Genomics advances for boosting aquaculture breeding programs in Spain

Hibridización de silúridos: una alternativa para la piscicultura con alto riesgo para la conservación de las especies nativas

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