Comprehensive Transcriptome and Metabolome Analysis Reveals Regulatory Network for Lignin Biosynthesis in Chicory Sprouting

Abstract

Purpose Chicory is consumed worldwide and is an important commercial crop. However, excess lignin deposition may reduce its quality. The molecular mechanisms underlying lignin remain poorly understood. To address this, an integrative analysis of the metabolome and transcriptome profiles was performed in chicory sprout at 3 different stages.Methods In this study, metabolites of the lignin biosynthesis pathway in chicory growth were determined by UPLC-MS/MS, moreover, multi-group sequencing and quantitative analysis of the transcriptome.Results A total of 706 metabolites were identified, with cinnamic acid, ferulic acid, Coniferaldehyde, and sinapaldehyde enriched during the growth of chicory sprouts. This suggested that these four metabolites may affect the growth of chicory sprouts. Transcriptome analysis demonstrated the expression of most of the differentially expressed genes (DEGs) involved in lignin biosynthesis was up-regulated during chicory growth.Conclusion Importantly, the metabolite and gene expression profiles were closely correlated during sprout development, especially in association with lignin biosynthesis. The results will serve as a reference for lignin biosynthesis in chicory and may also assist biologists in improving chicory quality.