Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages

Hao, Xinyuan; Yang, Yajun; Yue, Chuan; Wang, Lu; Horvath, David P.; Wang, Xinchao

doi:10.3389/fpls.2017.00553

Cited by 79 publications

(64 citation statements)

References 104 publications

(202 reference statements)

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“…However, the content of catechins usually varies with leaf stage, altitude, and season [2,3]. Moreover, several studies have illustrated various biosynthetic patterns for metabolites in C. sinensis, especially at the transcriptional level, using an Illumina HiSeq transcriptome sequencing technique [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Cao

et al. 2020

BMC Plant Biol

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Background: Leaves of the medicinal plant Ampelopsis grossedentata, which is commonly known as vine tea, are used widely in the traditional Chinese beverage in southwest China. The leaves contain a large amount of dihydromyricetin, a compound with various biological activities. However, the transcript profiles involved in its biosynthetic pathway in this plant are unknown. Results: We conducted a transcriptome analysis of both young and old leaves of the vine tea plant using Illumina sequencing. Of the transcriptome datasets, a total of 52.47 million and 47.25 million clean reads were obtained from young and old leaves, respectively. Among 471,658 transcripts and 177,422 genes generated, 7768 differentially expressed genes were identified in leaves at these two stages of development. The phenylpropanoid biosynthetic pathway of vine tea was investigated according to the transcriptome profiling analysis. Most of the genes encoding phenylpropanoid biosynthesis enzymes were identified and found to be differentially expressed in different tissues and leaf stages of vine tea and also greatly contributed to the biosynthesis of dihydromyricetin in vine tea. Conclusions: To the best of our knowledge, this is the first formal study to explore the transcriptome of A. grossedentata. The study provides an insight into the expression patterns and differential distribution of genes related to dihydromyricetin biosynthesis in vine tea. The information may pave the way to metabolically engineering plants with higher flavonoid content.

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Section: Introductionmentioning

confidence: 99%

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Cao

et al. 2020

BMC Plant Biol

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“…For instance, the buddy defect can be classified in class 4, 5 or 6 according to its intensity or combination of defects. One the other hand, the mechanisms of dormancy release and bud burst in deciduous trees are beginning to come to light 19,20,22,26,27,44 . Here we devised a basic dormancy index to compare our samples.…”

Section: Discussionmentioning

confidence: 99%

“…Some of them are known to be transported to the plant xylem through the sap as biological signals to trigger metabolic changes during the annual cycle 23,24 . Some studies investigated dormancy release through untargeted approach such as transcriptomic profiling analyses, improving knowledge about the genetic network involved 26,27 . However, little is known about the metabolic changes occurring during dormancy release in maple trees and their relationship with sap properties 28–30 .…”

Section: Introductionmentioning

confidence: 99%

A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality

Nguyen

Martin²,

Jain

et al. 2018

Preprint

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Maple sap is a complex nutrient matrix collected during Spring to produce maple syrup. The characteristics of sap change over the production period and its composition directly impacts syrup quality. This variability could in part be attributed to changes in tree metabolism following dormancy release, but little is known about these changes in deciduous trees. Therefore, understanding the variation in sap composition associated with dormancy release could help pinpoint the causes of some defects in maple syrup. In particular, a defect known as “buddy”, is an increasing concern for the industry. This off-flavor appears around the time of bud burst, hence its name. To investigate sap variation related to bud burst and the buddy defect, we monitored sap variation with respect to a dormancy release index (Sbb) and syrup quality. First, we looked at variation in amino acid content during this period. We observed a shift in amino acid relative proportions associated with dormancy release and found that most of them increase rapidly near the point of bud burst, correlating with changes in syrup quality. Second, we identified biological processes that respond to variation in maple sap by performing a competition assay using the barcoded Saccharomyces cerevisiae prototroph deletion collection. This untargeted approach revealed that the organic sulfur content may be responsible for the development of the buddy off-flavor, and that dormancy release is necessary for the appearance of the defect, but other factors such as microbial activity may also be contributing.

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“…Although the focus of this mini-review is not on bud dormancy, it is relevant to note that several studies have associated the genetic regulation of chilling requirement and dormancy with Dormancy Associated MADs-box ( DAM ) genes in peach ( Bielenberg et al, 2008 ; Li et al, 2009 ) apple ( Wu et al, 2017a ), pear ( Saito et al, 2013 ), apricot ( Sasaki et al, 2011 ), leafy spurge ( Euphorbia esula ) ( Horvath et al, 2010 ), kiwifruit ( Wu et al, 2011 , 2017b ), and tea plant ( Camellia sinensis ) ( Hao et al, 2017 ). Bud-dormancy associated candidate genes have also been identified in blackcurrant ( Ribes nigrum ) by Hedley et al (2010) and Yordanov et al (2014) identified an early budbreak ( EBB ) gene in poplar that was an APETALA2/Ethylene responsive transcription factor responsible for early bud flush.…”

Section: Dormancy Status and Spring Phenology (Budbreak) In Relation mentioning

confidence: 99%

Cold Hardiness in Trees: A Mini-Review

2018

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Significant advances have been made in our understanding of the regulation of cold hardiness. The existence of numerous biophysical and biochemical adaptive mechanisms in perennial woody plants and the complexity their regulation has made the development of methods for managing and improving cold hardiness in perennial woody plants has been very difficult. This may be partially attributed to viewing cold hardiness as a single dimensional response, rather than as a complex phenomenon, involving different mechanisms (avoidance and tolerance), different stages (mid-winter vs. late winter), and having an intimate overlap with the genetic regulation of dormancy. In particular separating the molecular regulation of cold hardiness from growth processes has been challenging. ICE and C-repeat binding factor (CBF), transcription factors (Inducer of CBF expression and CRT-binding factor) have been shown to be an important aspect in the regulation of cold-induced gene expression. Evidence has emerged, however, that they are also intimately involved in the regulation of growth, flowering, dormancy, and stomatal development. This evidence includes the presence of CBF binding motifs in genes regulating these processes, or through cross-talk between the pathways that regulate them. Recent changes in climate that have resulted in erratic episodes of unseasonal warming followed by more seasonal patterns of low temperatures has also highlighted the need to better understand the genetic and molecular regulation of deacclimation, a topic of research that is only more recently being addressed. Environmentally-induced epigenetic regulation of stress responses and seasonal processes such as cold acclimation, deacclimation, and dormancy have been documented but are still poorly understood. Advances in the ability to efficiently generate large DNA and RNA datasets and genetic transformation technologies have greatly increased our ability to explore the regulation of gene expression and explore genetic diversity. Greater knowledge of the interplay between epigenetic and genetic regulation of cold hardiness, along with the application of advanced genetic analyses, such as genome-wide-association-studies (GWAS), are needed to develop strategies for addressing the complex processes associated with cold hardiness in woody plants. A cautionary note is also indicated regarding the time-scale needed to examine and interpret plant response to freezing temperatures if progress is to be made in developing effective approaches for manipulating and improving cold hardiness.

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Comprehensive Transcriptome Analyses Reveal Differential Gene Expression Profiles of Camellia sinensis Axillary Buds at Para-, Endo-, Ecodormancy, and Bud Flush Stages

Cited by 79 publications

References 104 publications

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

Annotation of genes involved in high level of dihydromyricetin production in vine tea (Ampelopsis grossedentata) by transcriptome analysis

A systems biology approach to explore the impact of maple tree dormancy release on sap variation and maple syrup quality

Cold Hardiness in Trees: A Mini-Review

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