Comprehensive structural analysis of polyphenols and their enzymatic inhibition activities and antioxidant capacity of black mulberry (Morus nigra L.)

Huo, Junwei; Ni, Yana; Li, Dalong; Qiao, Jinli; Huang, Dejian; Sui, Xiaonan; Zhang, Yan

doi:10.1016/j.foodchem.2023.136605

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…Numerous studies indicate that these perishable fruits directly contribute to their noteworthy health advantages. Previous studies on its antioxidant, anti-inflammatory, and brain-boosting properties found a clear correlation between these properties and high phenolic component concentrations, especially when anthocyanins were present [39]. Considering these facts, the current study examined the capacity of R. fruticosus, R. ulmifolius, and M. nigra phenolic-rich extracts to scavenge DPPH • , • NO, and O 2…”

Section: Biological Potentialmentioning

confidence: 94%

“…DPPH is a synthetic radical; its test is frequently carried out because of its stability and simplicity. This makes it possible to broadly evaluate various extracts' and individual The capacity of M. nigra and R. fruticosus to reduce DPPH • has already been reported [26,39,40]. Indeed, Huo and co-workers revealed that crude extracts of Chinese black M. nigra were the most effective (12.52 µmol Trolox equivalent (TE)/g dw), followed by the anthocyanin fraction (87.25 µmol TE/g dw) and the non-anthocyanin fraction (113.52 µmol TE/g dw) [39].…”

Section: Antioxidant Activitymentioning

confidence: 98%

“…This makes it possible to broadly evaluate various extracts' and individual components' antioxidant activity. It is based on the transformation from violet to yellow that occurs when a tested substance or extract donates hydrogen to DPPH • , neutralising it [39]. All tested extracts exhibited dose-dependent effects against DPPH • (Table 2 and Figure 3A).…”

Section: Antioxidant Activitymentioning

confidence: 99%

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The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth

Martins,

Rodrigues,

Flores-Félix

et al. 2024

Nutrients

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Currently, a clear interest has been given to berries due to their richness in active metabolites, including anthocyanins and non-coloured phenolics. Therefore, the main aim of the present work is to investigate the phenolic profile, antioxidant abilities, and antiproliferative effects on normal human dermal fibroblasts (NHDF) and human colon carcinoma cell line (Caco-2) cells of phenolic-rich extracts from three red fruits highly appreciated by consumers: two species of blackberries (Rubus fruticosus and Rubus ulmifolius) and one species of mulberry (Morus nigra). A total of 19 different phenolics were identified and quantified by HPLC-DAD-ESI/MSn and HPLC-DAD, respectively. Focusing on the biological potential of the phenolic-rich extracts, all of them revealed notable scavenging abilities. Concerning the antiproliferative properties, R. fruticosus presented a cytotoxic selectivity for Caco-2 cells compared to NHDF cells. To deeper explore the biological potential, combinations with positive controls (ascorbic acid and 5-fluorouracil) were also conducted. Finally, the obtained data are another piece of evidence that the combination of phenolic-rich extracts from natural plants with positive controls may reduce clinical therapy costs and the possible toxicity of chemical drugs.

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Section: Biological Potentialmentioning

confidence: 94%

Section: Antioxidant Activitymentioning

confidence: 98%

Section: Antioxidant Activitymentioning

confidence: 99%

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The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth

Martins,

Rodrigues,

Flores-Félix

et al. 2024

Nutrients

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“…Compound 7 with a molecular ion at m/z [M − H] − 447 was identified as quercitrin, which first undergoes neutral depletion of [M-H-146] − from rhamnoside, yielding fragment ions of m/z 301, the quercetin ion. The quercetin ion proceeds undergoing Retro-Diels-Alder cleavage, producing fragment ions of m/z 179 and m/z 163 [18,19]. In addition, flavonoid molecular ions can be identified by the loss of neutral fragments (H 2 O, CO, and CO 2 ).…”

Section: Identification Of Flavonoid Compounds By Uplc-q-tof-msmentioning

confidence: 99%

Natural Deep Eutectic Solvent-Based Ultrasound-Assisted Extraction of Flavonoids from Fagopyrum tataricum Bran

Xu,

Da,

et al. 2024

Separations

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In this study, eleven kinds of flavonoids were identified from F. tataricum bran (FTB) by UPLC-Q-TOF-MS, and HPLC-DAD analysis revealed that four compounds, including rutin, quercetin, kaempferol, and nicotiflorin, were the most significant components. Subsequently, natural deep eutectic solvent-based ultrasound-assisted extraction (NADES-UAE) was employed to extract flavonoids from FTB. Among the six kinds of NADES prepared, choline chloride–ethylene glycol (ChCl-EG) was identified as a promising candidate for extracting flavonoids due to its superior extraction performance. The extraction conditions were statistically investigated using response surface methodology conducted by Box-Behnken design (BBD). The optimal operational conditions were as follows: ultrasonic time 268 s, ultrasonic temperature 76 °C, and liquid–solid ratio 43 mL/g, which resulted in a high total flavonoid yield of 40.29 mg/g. Afterwards, the efficient extraction mechanism of NADES-UAE was comprehensively explored through FT-IR spectra, COSMO model, and microstructural analysis. In conclusion, NADES-UAE extraction is considered a green, efficient, and sustainable method for FTB flavonoids.

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“…The action of polyphenols in the food matrix can be activated and used according to many options [20][21][22][23][24]. The literature on this subject indicates that polyphenols can directly inhibit and scavenge free radicals from the matrix or may act indirectly by affecting the enzymes responsible for the formation of radicals [21][22][23][24]. Research has repeatedly confirmed that the use of polyphenols in food can inhibit oxidative changes in animal and vegetable fats [25].…”

Section: Characteristics Of Hop Extractsmentioning

confidence: 99%

Antioxidant Activity of Humulus lupulus Phenolic Hop Extracts in Creating a New Pâté: An Element Affecting Fat Stability and Microbiological Quality during Storage

Bilska,

Kobus-Cisowska,

Wojtczak

et al. 2024

Molecules

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The aim of this study was to evaluate the effect of hop extracts on changes in the primary and secondary fat oxidation products, physicochemical properties, and microbiological quality of pâté-type offal sausages obtained through the partial replacement of animal fat with vegetable fat. This study demonstrated that the extraction efficiency varied among hop cone varieties, with the highest efficiency observed for the Lubelski variety and the lowest for the Magnum variety. The phenolic compound content was higher in the Magnum cones (2.74 ± 0.11 mg/g dry matter) compared to the Lubelska cones (2.27 ± 0.05 mg/g of product). Additionally, the DPPH radical scavenging activity was greater in the extract from the Magnum cones (4.21 ± 0.09 mg TE/g d.w.) than in the extract from the Lublelski cones (3.87 ± 0.05 mg TE/ g d.w.). Similarly, the extracts from the Lubelski cones exhibited a higher antiradical activity against the ABTS radical compared to the extract from Magnum cones. Throughout storage, a significant increase in the pH value was observed in the control sample and in the samples with a 20% replacement of animal fat with rapeseed oil and Magnum hop extract. However, the addition of Lubelski hop extract resulted in a decrease in the pH value during the 15-day storage period. The samples with a 20% replacement of animal fat with rapeseed oil and 0.1% Lubelski hop extract showed the least changes in water activity during storage. The samples with a 20% replacement of animal fat with rapeseed oil and the addition of 0.2% Lubelski hop extract had the lowest peroxide value and TBARS index throughout the storage period. The addition of hop extract inhibited the growth of the total number of microorganisms in the tested sausages. In the samples with a 20% replacement of animal fat with rapeseed oil, the content of aerobic microorganisms, compared to the control sample, was statistically significantly lower.

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Comprehensive structural analysis of polyphenols and their enzymatic inhibition activities and antioxidant capacity of black mulberry (Morus nigra L.)

Cited by 11 publications

References 39 publications

The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth

The Effect of Phenolic-Rich Extracts of Rubus fruticosus, R. ulmifolius and Morus nigra on Oxidative Stress and Caco-2 Inhibition Growth

Natural Deep Eutectic Solvent-Based Ultrasound-Assisted Extraction of Flavonoids from Fagopyrum tataricum Bran

Antioxidant Activity of Humulus lupulus Phenolic Hop Extracts in Creating a New Pâté: An Element Affecting Fat Stability and Microbiological Quality during Storage

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