Comprehensive speciation of selenium in selenium-rich yeast

Bierła, Katarzyna; Szpunar, Joanna; Yiannikouris, Alexandros; Łobiński, Ryszard

doi:10.1016/j.trac.2012.08.006

Cited by 88 publications

(84 citation statements)

References 22 publications

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“…Se-enriched yeast is currently the primary Se dietary supplement, where Se is present mostly as SeMet. 35 Several minor organic species have been reported, such as methylselenol, SeCys, selenohomocysteine, selenoadenosine and others at trace levels. 35 …”

Section: Se Supplementationmentioning

confidence: 99%

Selenium biochemistry and its role for human health

Roman¹,

Jitaru²,

Barbante³

2014

Metallomics

629

451

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Despite its very low level in humans, selenium plays an important and unique role among the (semi)metal trace essential elements because it is the only one for which incorporation into proteins is genetically encoded, as the constitutive part of the 21st amino acid, selenocysteine. Twenty-five selenoproteins have been identified so far in the human proteome. The biological functions of some of them are still unknown, whereas for others there is evidence for a role in antioxidant defence, redox state regulation and a wide variety of specific metabolic pathways. In relation to these functions, the selenoproteins emerged in recent years as possible biomarkers of several diseases such as diabetes and several forms of cancer. Comprehension of the selenium biochemical pathways under normal physiological conditions is therefore an important requisite to elucidate its preventing/therapeutic effect for human diseases. This review summarizes the most recent findings on the biochemistry of active selenium species in humans, and addresses the latest evidence on the link between selenium intake, selenoproteins functionality and beneficial health effects. Primary emphasis is given to the interpretation of biochemical mechanisms rather than epidemiological/observational data. In this context, the review includes the following sections: (1) brief introduction; (2) general nutritional aspects of selenium; (3) global view of selenium metabolic routes; (4) detailed characterization of all human selenoproteins; (5) detailed discussion of the relation between selenoproteins and a variety of human diseases.

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Section: Se Supplementationmentioning

confidence: 99%

Selenium biochemistry and its role for human health

Roman¹,

Jitaru²,

Barbante³

2014

Metallomics

629

451

View full text Add to dashboard Cite

show abstract

“…Since L-SeMet is the predominant selenocompound incorporated into proteins of Se yeast, the panel concluded that there was no reason to assume that free L-SeMet would result in an essentially different deposition pattern [49]. However, as SeMet is incorporated randomly into proteins as a substitute for the sulphur analogue Met [25,37,50], the degree of expression of particular proteins depends on the yeast strain and the fermentation process [17] as clearly evidenced in Table 1.…”

Section: Determination Of Inter-batch and Inter-product Protein Contementioning

confidence: 99%

“…Three different isoforms of glyceraldehyde-3-phosphate dehydrogenase (Tdh1, Tdh2 and Tdh3) were also identified. GAPDH is ubiquitous in yeast and has previously been reported to incorporate SeMet randomly within the protein [17]. In general, yeast supports a high degree of SeMet incorporation, by replacing about 30 % of all Met with SeMet [24,25,50].…”

Section: Determination Of Inter-batch and Inter-product Protein Contementioning

confidence: 99%

“…Protein identities were validated using UniProt, and Gene Ontology classification was performed to define the potential biological role of the identified proteins and their relevance to animal health [16]. Characterisation of Se yeast in this way provides a unique fingerprint of origin and accounts for fate of Se during the production process, which is paramount importance for regulatory and safety agencies [17]. The Se-enriched yeast certified reference material (SELM-1) was used for the calibration of instruments and evaluation of methods for the determination of SeMet, Met and total Se in yeast.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Comparison of Commercial Selenium Yeast Preparations

Fagan

Owens

Ward

et al. 2015

Biol Trace Elem Res

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The trace mineral selenium (Se) is an essential element for human and animal nutrition. The addition of Se to the diet through dietary supplements or fortified food/feed is increasingly common owing to the often sub-optimal content of standard diets of many countries. Se supplements commercially available include the inorganic mineral salts such as sodium selenite or selenate, and organic forms such as Se-enriched yeast. Today, Se yeast is produced by several manufacturers and has become the most widely used source of Se for human supplementation and is also widely employed in animal nutrition where approval in all species has been granted by regulatory bodies such as the European Food Safety Authority (EFSA). Characterisation and comparison of Se-enriched yeast products has traditionally been made by quantifying total selenomethionine (SeMet) content. A disadvantage of this approach, however, is that it does not consider the effects of Se deposition on subsequent digestive availability. In this study, an assessment was made of the water-soluble extracts of commercially available Se-enriched yeast samples for free, peptide-bound and total water-soluble SeMet. Using LC-MS/MS, a total of 62 Se-containing proteins were identified across four Se yeast products, displaying quantitative/ qualitative changes in abundance relative to the certified reference material, SELM-1 (P value <0.05; fold change ≥2). Overall, the study indicates that significant differences exist between Se yeast products in terms of SeMet content, Secontaining protein abundance and associated metabolic pathways.

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“…In fact, the Se-containing biomolecules involving in metabolic process of Se-enriched yeast were studied and many new Se species were discovered [28][29][30][31][32][33] . Thus the Se-enriched yeast was used to demonstrate the feasibility of the proposed strategy.…”

Section: Potential Selenoprotein (Peptide) In Se-enriched Yeast Watermentioning

confidence: 99%

Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide

Yang

Wang

2015

Chinese Journal of Analytical Chemistry

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An endogenous element-label plus exogenous element-tag strategy was proposed for inductively coupled plasma-mass spectrometry (ICP-MS) to screen and discriminate a family of ultratrace but biologically important biomolecules. The feasibility of this Se signals out of fifteen Se-containing species using RPLC/ICP-MS, providing valuable information for further identification using a high-resolution structure-selective mass spectrometer. This endogenous element-label plus exogenous element-tag dual-element approach implies that ICP-MS is not only able to quantify targeted proteins (peptides) but also helpful to discover unknown ones during a discovery-based proteomic study.

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Comprehensive speciation of selenium in selenium-rich yeast

Cited by 88 publications

References 22 publications

Selenium biochemistry and its role for human health

Selenium biochemistry and its role for human health

Biochemical Comparison of Commercial Selenium Yeast Preparations

Se-Hg Dual-element Labeling Strategy for Selectively Recognizing Selenoprotein and Selenopeptide

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